Influence of manufacturing processes on cell surface properties of probiotic strain Lactobacillus rhamnosus Lcr35®

Abstract

The influence of the industrial process on the properties of probiotics, administered as complex manufactured products, has been poorly investigated. In the present study, we comparatively assessed the cell wall characteristics of the probiotic strain Lactobacillus rhamnosus Lcr35® together with three of its commercial formulations with intestinal applications. Putative secreted and transmembrane-protein-encoding genes were initially searched in silico in the genome of L. rhamnosus Lcr35®. A total of 369 candidate genes were identified which expressions were followed using a custom Lactobacillus DNA chip. Among them, 60 or 67 genes had their expression either upregulated or downregulated in the Lcr Restituo® packet or capsule formulations, compared to the native Lcr35® strain. Moreover, our data showed that the probiotic formulations (Lcr Lenio®, Lcr restituo® capsule and packet) showed a better capacity to adhere to intestinal epithelial Caco-2 cells than the native Lcr35® strain. Microbial (MATS) tests showed that the probiotic was an electron donor and that they were more hydrophilic than the native strain. The enhanced adhesion capacity of the active pharmaceutical ingredients (APIs) to epithelial Caco-2 cells and their antipathogen effect could be due to this greater surface hydrophilic character. These findings suggest that the manufacturing process influences the protein composition and the chemical properties of the cell wall. It is therefore likely that the antipathogen effect of the formulation is modulated by the industrial process. Screening of the manufactured products' properties would therefore represent an essential step in evaluating the effects of probiotic strains.

Probiotic characteristics of exopolysaccharides-producingLactobacillusisolated from some traditional Malaysian fermented foods

“…boza). Moreover, Nivoliez et al (2015) obtained auto-aggregation values ranging from 16% to 31% and 12% to 21% for the probiotic strain L. rhamnosus Lcr35 after 3 and 5 h, respectively. Therefore, it is difficult to articulate a standard value for a high auto-aggregation value for Lactobacillus strains.…”

“…This includes the resistance to bile and low pH, gastric juices, and the ability to adhere and colonize gut epithelial (Bao et al, 2010;Del Piano et al, 2006). Furthermore, this also includes autoaggregation, co-aggregation, as well as microbial adhesion to solvents (MATS) (Nivoliez et al, 2015). Probiotics should also be resistant to antibiotics (Pinto, Franz, Schillinger, & Holzapfel, 2006).…”

Identification of sulfur components enhancing the anti-Candida effect of Lactobacillus rhamnosus Lcr35

“…The beneficial properties of microorganisms are usually evaluated with native culture strains. However, LBM are often administered as complex manufactured formulated products and positive impacts of the manufacturing process have been previously demonstrated in several studies [22][23][24] . In this study, we demonstrated that the vaginal formulation of Lactobacillus rhamnosus Lcr35, GYNOPHILUS, was able to kill C. albicans ATCC 10231 after 48 h, whereas the native strain alone only reduced the pathogen viability by 3 log 10 (CFU/ml) over the same length of time 22 .…”

Comparative phase I randomized open-label pilot clinical trial of Gynophilus® (Lcr regenerans®) immediate release capsules versus slow release muco-adhesive tablets

“…The LBP Gynophilus ® is vaginally delivered and is indicated to prevent recurrent VVC and BV by restoring vaginal health [ 11 – 13 ]. This LBP contains L. rhamnosus Lcr35 ® , which was previously reported to have potent antimicrobial properties [ 18 , 19 , 29 – 32 ]. Gynophilus ® (Lcr regenerans ® ) LBP formulated in vaginal capsules was shown to be well-tolerated and significantly enhanced the quality of life of women after treatment [ 13 ].…”