Influence of Macrophage Infiltration of Herniated Disc Tissue on the Production of Matrix Metalloproteinases Leading to Disc Resorption

Doita, Minoru; Kanatani, Takako; Ozaki, Takuma; Matsui, Noriko; Kurosaka, Masahiro; Yoshiya, Shinichi

doi:10.1097/00007632-200107150-00004

Cited by 129 publications

(87 citation statements)

References 25 publications

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“…Subsequently, various inflammatory chemical factors secreted from herniated NP, including tumor necrosis factor (TNF)-α (Weiler et al, 2005;Le Maitre et al, 2007), interleukin (IL)-1β (Le Maitre et al, 2007) and nitric oxide (NO) (Katsuno et al, 2008), have been implicated as causes of sciatica (McCarron et al, 1987;Geiss et al, 2007). Further, the production of matrix metalloproteinases (MMPs) has been implicated in the resorption of the herniated NP (Doita et al, 2001). Bobechko and Hirsh (1965) and Gertzbein et al (1975) reported that herniated NP tissue is recognized as a foreign antigen that induces an autoimmune response producing inflammation.…”

Section: Introductionmentioning

confidence: 99%

Primary immune system responders to nucleus pulposus cells: evidence for immune response in disc herniation

Murai

Sakai²,

Nakamura³

et al. 2010

eCM

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Although intervertebral disc herniation and associated sciatica is a common disease, its molecular pathogenesis is not well understood. Immune responses are thought to be involved. This study provides direct evidence that even nondegenerated nucleus pulposus (NP) cells elicit immune responses. An in vitro colony forming inhibition assay demonstrated the suppressive effects of autologous spleen cells on NP cells and an in vitro cytotoxicity assay showed the positive cytotoxic effects of natural killer (NK) cells and macrophages on NP cells. Non-degenerated rat NP tissues transplanted into wild type rats and immunedeficient mice demonstrated a significantly higher NP cell survival rate in immune-deficient mice. Immunohistochemical staining showed the presence of macrophages and NK cells in the transplanted NP tissues. These results suggest that even non-degenerated autologous NP cells are recognized by macrophages and NK cells, which may have an immunological function in the early phase of disc herniation. These findings contribute to understanding resorption and the inflammatory reaction to disc herniation.

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Section: Introductionmentioning

confidence: 99%

Primary immune system responders to nucleus pulposus cells: evidence for immune response in disc herniation

Murai

Sakai²,

Nakamura³

et al. 2010

eCM

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“…Previous studies have provided evidence for the neovascularization and infiltration of macrophages and other inflammatory cells into herniated disc tissues (12,49). In the current study, IVDs from patients with NCDH had significantly more neovascularization and granulation tissue than patients with CDH.…”

Section: Discussionmentioning

confidence: 99%

Role of interleukin-17 in chondrocytes of herniated intervertebral lumbar discs

Tian¹,

Li²,

Fu³

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. Lumbar disc herniation (LDH) is a common cause of lumbosacral radiculopathy. An autoimmune response to a herniated nucleus pulposus (NP) has been suggested to play an important role in the initiation of radiculopathy. Interleukin-17 (IL-17) is a cytokine associated with inflammation and autoimmunity. The presence of IL-17 has been studied in patients with LDH; however, extensive investigation into the expression of IL-17 in different disc pathologies of LDH has not yet been conducted. The aim of the present study was to investigate the role of neovascularization and hypertrophic chondrocytes in herniated intervertebral lumbar discs. Fifty-two intervertebral lumbar disc specimens were extracted from 46 patients with LDH and were subsequently classified as either contained or non-contained disc herniation (CDH and NCDH, respectively). The specimens were stained with hematoxylin and eosin or toluidine blue, or were immunostained with polyclonal antibodies to IL-17 using the streptavidin-peroxidase method. The neovascular tissue and staining results were graded to establish the histological differences between the two herniation types. The intervertebral discs (IVDs) obtained from patients with NCDH showed significantly more neovascularization and granulation tissue than the discs obtained from patients with CDH (P<0.05). Furthermore, hypertrophic chondrocytes were more abundant in the NCDH specimens than in the CDH specimens (P<0.05). Similarly, the number of IL-17-immunoreactive cells was significantly higher in the NCDH specimens than that in the CDH specimens (P<0.01).In conclusion, local inflammation and autoreactive immune activation may play an important role in the pathogenesis of LDH. These results also suggest a role of chondrocytes in the repair of herniated IVDs.

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“…The inflammatory and immune activation profile exhibited by a herniated disc may be distinct from that observed in the IVD degeneration and may differ further among different extents of herniation from protrusion through sequestration. Histologic evaluation of herniated disc tissue has revealed prominent infiltration of inflammatory cells, most markedly macrophages [6][7][8][9][10][11]. Various inflammatory cells, proinflammatory cytokines, antibodies, and immunoglobulins have been identified in and around herniated lumbar disc tissue.…”

Section: Introductionmentioning

confidence: 99%

“…Various inflammatory cells, proinflammatory cytokines, antibodies, and immunoglobulins have been identified in and around herniated lumbar disc tissue. Recently, it has been reported that lumbar disc tissue may be another potential immune-privileged site in the human body [6][7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

TH1 and TH2 Cytokines Production and NK Cell Level Assessment in Peripheral Blood of Patients with DDH

Akyol

Hancı

2012

Indian J Surg

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In this study, our aim is; if the studies will quide us in peripheral blood, for the changes in inflammatory cytokine levels we defined before DDH tissue. Twenty-six patients were suggestive of lumbar DDH were included in this study. Control subjects included 14 autopsy cases. From each patient, disc tissues and peripheral blood samples for plasma were collected during the surgery. For the controls, disc samples and blood for plasma by intracardiac puncture were obtained during autopsy. The Flow Cytometry was used to obtain the lymphocyte CD56 (NK). The Luminex was used to obtain IL-2, IL-4, IL-10, IL-12, IFN-gamma, in both plasma and disc tissues. The results were compared between the two groups. Comparing the two groups regarding plasma demonstrated that IL-2, IL-4, IL-12, IFNgamma were significantly higher than in patients than those of the controls. Likewise, tissue levels of IL-2, IL-4, IL-10, IL-12, TNF-alpha, CD56 were found to be significantly higher in the patients. With respect to the comparison between the plasma disc samples in the patients, plasma showed significant higher levels of IL-2, IL-12 on the other hand IL-4 was found to be significantly higher in the disc samples. Findings suggest that only tissue samples responses in occurring but not blood samples. We don't think our results in peripheral blood will guide us specifically in DDH.

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Influence of Macrophage Infiltration of Herniated Disc Tissue on the Production of Matrix Metalloproteinases Leading to Disc Resorption

Cited by 129 publications

References 25 publications

Primary immune system responders to nucleus pulposus cells: evidence for immune response in disc herniation

Primary immune system responders to nucleus pulposus cells: evidence for immune response in disc herniation

Role of interleukin-17 in chondrocytes of herniated intervertebral lumbar discs

TH1 and TH2 Cytokines Production and NK Cell Level Assessment in Peripheral Blood of Patients with DDH

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