Influence of Luminol Doping of Poly(o-phenylenediamine) on the Spectral, Morphological, and Fluorescent properties: A Potential Fluorescent Marker for Early detection and Diagnosis of Leishmania donovani

Abstract

There has been a steady progress in the development of doped conjugated polymers to remarkably improve their photo physical properties for their application as biomarkers. With a view to enhance the spectral, morphological, and photo physical properties of poly(o-phenylenediamine) (POPD), the present work reports the synthesis of poly(o-phenylenediamine) and doping of this polymer using luminol. The formation of luminol-doped POPD was confirmed by infrared and ultraviolet-visible spectroscopies and X-ray diffraction studies. The energy band gap values and oscillator strength of luminol in acidic, basic, and neutral media were computed by density functional theory calculations using the B3LYP/6-31G (d) basis set and were compared with experimental data. The luminol doped POPDs show significant in vitro anti-leishmanial activity. Live cell imaging also proved that these molecules bind with the organelle of Leishmania also. These luminol doped POPDs were found non-toxic at the used concentrations on THP-1 derived human macrophage cells through methyl tetrazolium (MTT) assay. The results revealed that luminol doped POPDs were potentially non-toxic to human cells though exhibited immense potential to be used as a fluorescent marker to label Leishmania donovani for diagnostic and other studies.

Experimental and Theoretical Studies of Novel Azo Benzene Functionalized Conjugated Polymers: In-vitro Antileishmanial Activity and Bioimaging

“…The experimental and theoretical spectra were observed to be in good agreement which confirmed the proposed chmical structure of the polymers. www.nature.com/scientificreports www.nature.com/scientificreports/ Upon excitation at 480 nm, the fluorescence spectra of PANI-AB and PPd-AB exhibited emission maxima at 570 nm while PNA-AB and PLu-AB showed broad emission humps spanning between 500-570 nm corresponding to S 1 →S o transition 22,27 , Fig. 4(a).…”

Microwave-assisted facile synthesis of poly(luminol-co-phenylenediamine) copolymers and their potential application in biomedical imaging

“…The studies pertaining to cell proliferation, viability and imaging were carried out as per reported protocols. 31,40 Results and discussion…”

“…29,30 Luminol was chosen as a co-monomer because it is commonly used as a chemiluminescence reagent in forensic medicine for selective detection of blood stains, but no work has been reported on the chemical copolymerization of luminol. [31][32][33][34] O-phenylenediamine (OPD) on the other hand, is an extensively explored polyaniline (PANI) derivative which exhibits outstanding photo-physical properties and fairly good water solubility. 35,36 Copolymerization of the two monomers was carried out with a view to tailor the opto-electronic and electrochemical properties of copolymers by varying the comonomer ratio.…”

Impact of vanadium‐, sulfur‐, and dysprosium‐doped zinc oxide nanoparticles on various properties of PVDF/functionalized‐PMMA blend nanocomposites: Structural, optical, and morphological studies

“…The fluorescence emission spectrum of pristine PVDF/fun‐PMMA blends and their nanocomposites in DMF were recorded upon excitation of the solutions at λ exc = 380 nm, depicted in Figure . The emission spectra of pristine PVDF/fun‐PMMA shown three different patterns of emission under excitation wavelength at relatively very high intensity, showed that the number of decaying excited state polymer molecules were at higher value, or, HOMOs and LUMOs were symmetrically disposed . The all samples exhibited an emission peak around 407 nm may be correspond to the PVDF and at around 431 nm may correspond to functionalized‐PMMA revealing a noticeable blueshift with the incorporation of nanosized undoped/doped ZnO; while at low intensity, 457 nm show the functionalized group present in the blended polymers which show noticeable redshift with incorporation of nanoparticles.…”