Influence of low‐level laser associated with osteogenic proteins recombinant human BMP‐2 and Hevea brasiliensis on bone repair in Wistar rats

Abstract

This study analyzed the newly formed bone tissue after application of recombinant human BMP-2 (rhBMP-2) and P-1 (extracted from Hevea brasiliensis) proteins, 2 weeks after the creation of a critical bone defect in male Wistar rats treated or not with a low-intensity laser (GaAlAs 780 nm, 60 mW of power, and energy density dose of 30 J/cm(2)). The animals were divided into two major groups: (1) bone defect plus low-intensity laser treatment and (2) bone defect without laser irradiation. The following subgroups were also analyzed: (a) 5 μg of pure rhBMP-2; (b) 5 μg of pure P-1 fraction; (c) 5 μg of rhBMP-2/monoolein gel; (d) 5 μg of P-1 fraction/monoolein gel; (e) pure monoolein gel. Comparisons of the groups receiving laser treatment with those that did not receive laser irradiation show differences in the areas of new bone tissue. The group treated with 5 μg of rhBMP-2 and laser irradiation was not significantly different (P >0.05) than the nonirradiated group that received the same treatment. The irradiated, rhBMP-2/monoolein gel treatment group showed a lower area of bone formation than the nonirradiated, rhBMP-2/gel monoolein treatment group (P < 0.001). The area of new bone tissue in the other nonirradiated and irradiated groups was not significantly different (P > 0.05). Furthermore, the group that received the 5 μg of rhBMP-2 application showed the greatest bone formation. We conclude that the laser treatment did not interfere with the area of new bone tissue growth and that the greatest stimulus for bone formation involved application of the rhBMP-2 protein.

A new heterologous fibrin sealant as scaffold to recombinant human bone morphogenetic protein-2 (rhBMP-2) and natural latex proteins for the repair of tibial bone defects

“…The results showed that P-1 increased the deposition of collagen fibers, whereas rhBMP-2 significantly increased bone neoformation at the defect site. The latter was considered to be more effective in the process of bone consolidation (Iyomasa et al, 2012). In the present study, the new fibrin sealant was used as a scaffold for cell adhesion and proliferation and was also found to be a good carrier of bone proteins.…”

“…In the present study, the fibrin sealant was combined with two promising osteogenic factors, rhBMP-2 and protein P-1 extracted from the latex of the rubber tree H. brasiliensis, in order to determine the osteoconductive potential of this combination in bone healing. The osteoinductive potential of pure and recombinant BMP-2 and its applicability to the healing of bone defects have been reported (Iyomasa et al, 2012). In parallel to BMPs, other natural substances have been explored in studies on bone regeneration, including fraction P-1 extracted from the rubber tree which exerts osteoconductive activity through the acceleration of bone neoformation, particularly in early stages of the healing process, as a result of its angiogenic properties and biocompatibility (Balabanian et al, 2006).…”

How to improve the survival rate of implants after radiotherapy for head and neck cancer?

“…Springer et al [49] studied the application of BMP in the irradiated mandible and found that BMP could cooperate with bFGF and result in predictable bone generation. Other studies also identified that BMP could enhance the generation and repair of bones after radiotherapy [50,51]. …”

Oral tissue response to ovine grafting biomaterial: Morphological and morphometric study using scanning electron and light microscopy tissue response to ovine grafting biomaterial

“…There are many consolidated reports of the application of bone biomaterials of xenogeneic origin (Iyomasa et al, 2012), especially bovine, for oral rehabilitation (Bornstein et al, 2008; Kim et al, 2009; Pieri et al, 2008; Simunek et al, 2008) and orthopedic purposes (Chiang et al, 2008; Rosito et al, 2008; Schultheiss et al, 2005). This vast scientific literature about bovine grafting biomaterials even justifies its use as standard during experimentation with new synthetic materials (Kim and Kim, 2008).…”

“…Thus, although it may seem logical that other farm animals may also be used for the extractions of bone grafting biomaterial bases, this does not usually happen. Even though sheep may present several advantages over cattle (such as lower demand for space and shorter periods for reproduction, breeding, and slaughtering), such animals are used only as in vivo models in experimental bone grafting studies (Cheng et al, 2005; Collignon et al, 1997; Giavaresi et al, 2008; Iyomasa et al, 2012; Paknejad et al, 2008; Pereira et al, 2011; Santoni et al, 2008; Swennen et al, 2005; Vlaminck et al, 2008) or in vitro with ovine origin cells (Rhodes et al, 2004; Schmitt et al, 2008). Also, it has already been demonstrated that ovine bone has similar characteristics to human bone (Grimm et al, 2001).…”