Influence of low dose naltrexone on Raman assisted bone quality, skeletal advanced glycation end-products and nano-mechanical properties in type 2 diabetic mice bone

Shitole, Pankaj; Choubey, Abhinav; Mondal, Prosenjit; Ghosh, Rajesh

doi:10.1016/j.msec.2021.112011

Cited by 9 publications

(10 citation statements)

References 54 publications

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“…Similarly, some other studies also indicated the positive effect of naltrexone on bone mass , and bone formation and prevent bone loss . We have also identified that LDN can control Raman-based compositional bone quality parameters in T2DM mice bone . However, to the author’s knowledge, there is a dearth of an in-depth study to understand the effect of T2DM and LDN compound on bone properties such as fracture toughness, nano-properties, bone mineral crystal size, and bone composition.…”

Section: Introductionmentioning

confidence: 94%

“…The value for S was determined similar to the earlier published study. 10 Bone hardness (H) was identified using the following equation by the ratio of the maximum load (P max ) to the contact area (A)…”

Section: Mice Preparationmentioning

confidence: 99%

“…7−9 Recently, we have identified that LDN can control Raman-based compositional bone quality parameters in T2DM mice bone. 10 However, the influence of LDN on T2DM-affected bone properties in terms of fracture toughness, nano-Young's modulus, hardness, mineral crystal size, and bone composition is still unknown.…”

Section: Introductionmentioning

confidence: 99%

“…30 We have also identified that LDN can control Raman-based compositional bone quality parameters in T2DM mice bone. 10 However, to the author's knowledge, there is a dearth of an in-depth study to understand the effect of T2DM and LDN compound on bone properties such as fracture toughness, nano-properties, bone mineral crystal size, and bone composition. It has been anticipated that T2DM and LDN compounds might have an influence on bone properties at macro to nano level.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, low-dose naltrexone (LDN) exhibited enhanced glucose tolerance and insulin sensitivity in HFD-induced T2DM mice . Although naltrexone was synthesized as an orally active competitive opioid receptor antagonist, LDN exhibits paradoxical properties, including analgesia and anti-inflammatory actions, where LDN simultaneously has an antagonist effect on nonopioid receptors (TLR4) which have not been reported at larger dosages. − Recently, we have identified that LDN can control Raman-based compositional bone quality parameters in T2DM mice bone . However, the influence of LDN on T2DM-affected bone properties in terms of fracture toughness, nano-Young’s modulus, hardness, mineral crystal size, and bone composition is still unknown.…”

Section: Introductionmentioning

confidence: 99%

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LDN Protects Bone Property Deterioration at Different Hierarchical Levels in T2DM Mice Bone

et al. 2021

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Type 2 diabetes mellitus (T2DM) commonly affects bone quality at different hierarchical levels and leads to an increase in the risk of bone fracture. Earlier, some anti-diabetic drugs showed positive effects on bone mechanical properties. Recently, we have investigated that low-dose naltrexone (LDN), a TLR4 antagonist treatment, improves glucose tolerance in high-fat diet (HFD)-induced T2DM mice and also gives protection against HFD-induced weight gain. However, effects on bone are still unknown. In this study, the effects of LDN on the bone properties at different hierarchical levels in T2DM mice bone were investigated. In order to investigate these, four different groups of bone (divided based on diet and treatment) were considered in this present study. These are (a) normal control diet treated with saline water, (b) normal control diet treated with LDN, (c) HFD treated with saline water, and (d) HFD treated with LDN. Bone properties were measured in terms of fracture toughness, nano-Young's modulus, hardness, mineral crystal size, bone composition, and bulk mineral to matrix ratio. Results indicated that fracture toughness, nano-Young's modulus, and hardness were decreased in T2DM bone as compared to normal bone, and interestingly, treatment with the LDN increases these material properties in T2DM mice bone. Similarly, as compared to the normal bone, decrease in the mineral crystal size and bulk mineral-to-matrix ratio was observed in the T2DM bone, whereas LDN treatment protects these alterations in the T2DM mice bone. The bone size (bone geometry) was increased in the case of HFD-induced T2DM bone; however, LDN cannot protect to increase the bone size in the T2DM mice bone. In conclusion, LDN can be used to control the T2DM-affected bone properties at different hierarchical levels.

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Section: Introductionmentioning

confidence: 94%

Section: Mice Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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LDN Protects Bone Property Deterioration at Different Hierarchical Levels in T2DM Mice Bone

et al. 2021

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Physicochemical understanding of biomineralization by molecular vibrational spectroscopy: From mechanism to nature

et al. 2023

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The process and mechanism of biomineralization and relevant physicochemical properties of mineral crystals are remarkably sophisticated multidisciplinary fields that include biology, chemistry, physics, and materials science. The components of the organic matter, structural construction of minerals, and related mechanical interaction, etc., could help to reveal the unique nature of the special mineralization process. Herein, the paper provides an overview of the biomineralization process from the perspective of molecular vibrational spectroscopy, including the physicochemical properties of biomineralized tissues, from physiological to applied mineralization. These physicochemical characteristics closely to the hierarchical mineralization process include biological crystal defects, chemical bonding, atomic doping, structural changes, and content changes in organic matter, along with the interface between biocrystals and organic matter as well as the specific mechanical effects for hardness and toughness. Based on those observations, the special physiological properties of mineralization for enamel and bone, as well as the possible mechanism of pathological mineralization and calcification such as atherosclerosis, tumor micro mineralization, and urolithiasis are also reviewed and discussed. Indeed, the clearly defined physicochemical properties of mineral crystals could pave the way for studies on the mechanisms and applications.

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A survey on the mechanical properties of bone

Barekar,

Sarawade,

Kumar

2024

Multimed Tools Appl

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Influence of low dose naltrexone on Raman assisted bone quality, skeletal advanced glycation end-products and nano-mechanical properties in type 2 diabetic mice bone

Cited by 9 publications

References 54 publications

LDN Protects Bone Property Deterioration at Different Hierarchical Levels in T2DM Mice Bone

LDN Protects Bone Property Deterioration at Different Hierarchical Levels in T2DM Mice Bone

Physicochemical understanding of biomineralization by molecular vibrational spectroscopy: From mechanism to nature

A survey on the mechanical properties of bone

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