Influence of light quality on growth, secondary metabolites production and antioxidant activity in callus culture of Rhodiola imbricata Edgew

Kapoor, Sahil; Raghuvanshi, Rinky; Bhardwaj, Pushpender; Sood, Hemant; Saxena, Shweta; Chaurasia, O. P.

doi:10.1016/j.jphotobiol.2018.04.018

Cited by 101 publications

(65 citation statements)

References 66 publications

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“…These same factors can also influence the PDMC in vitro production. For example, Kapoor et al (2018) reported the influence of light quality on the production of accumulated biomass, growth rate, and concentration of the phenolic compound Salidroside in Rhodiola imbricata. Kapoor et al (2018) found the highest callus growth index (2.97) in red-light and the highest concentration of the total phenolic compound and Salidroside using blue light, in 21-day callus.…”

Section: Main Factors Affecting Pdmc Productionmentioning

confidence: 99%

“…For example, Kapoor et al (2018) reported the influence of light quality on the production of accumulated biomass, growth rate, and concentration of the phenolic compound Salidroside in Rhodiola imbricata. Kapoor et al (2018) found the highest callus growth index (2.97) in red-light and the highest concentration of the total phenolic compound and Salidroside using blue light, in 21-day callus. As they observed, in vitro systems are established using protocols based mainly in culture medium and environmental conditions adjusted to the different phases of the in vitro growth.…”

Section: Main Factors Affecting Pdmc Productionmentioning

confidence: 99%

“…In vitro micropropagation, followed by plantlet acclimatization and growth in greenhouse or in the field, is a promising strategy to produce plant secondary metabolites, especially in rare or endangered species, in those difficult to propagate (Kapoor et al, 2018) or of slow growth (Cardoso & Silva, 2013), and in species vulnerable to recurrent phytosanitary problems, such as those of vegetative propagation. In the later, the techniques used to produce virus-free plants may be helpful also to increase both plant biomass productivity and PDMC contents, as observed in Allium sativum.…”

Section: Main Factors Affecting Pdmc Productionmentioning

confidence: 99%

“…Callus induction and multiplication have been extensively used in PDMC in vitro production. It is an efficient approach to produce PDMCs in large scale when compared to other techniques, mainly because the in vitro callus induction is a straightforward and rapid system of cell multiplication (Kapoor et al, 2018). Besides, the factors that induce callogenesis are well studied (Ahmad et al, 2016), highly consistent, and used commercially already for some decades in tissue culture for other applications.…”

Section: Callus and Cell Suspension Culturementioning

confidence: 99%

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Advances and challenges on the in vitro production of secondary metabolites from medicinal plants

2019

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The production of secondary metabolites from medicinal plants, also called Plant-Derived Medicinal Compounds (PDMC), is gaining ground in the last decade. Concomitant to the increase in the knowledge about pharmacological properties of these compounds, horticultural plants are becoming the most important, sustainable and low-cost biomass source to obtain high-complex PDMCs to be used as medicaments. Biotechnological tools, including plant cell and tissue culture and plant genetic transformation, are increasingly being employed to produce high quality and rare PDMC under in vitro conditions. The proper use of these technologies requires studies in organogenesis to allow for better control of in vitro plant development and, thus, to the production of specific tissues and activation of biochemical routes that result in the biosynthesis of the target PDMCs. Either biotic or abiotic factors, called elicitors, are responsible for triggering the PDMC synthesis. In vitro techniques, when compared to the conventional cultivation of medicinal plants in greenhouse or in the field, have the advantages of (1) producing PDMCs in sterile and controlled environmental conditions, allowing better control of the developmental processes, such as organogenesis, and (2) producing tissues with high PDMC contents, due to the efficient use of different biotic and abiotic elicitors. Nevertheless, the process has many challenges, e.g., the establishment of step-by-step protocols for in vitro biomass and PDMC production, both involving and being affected by many factors. Other limitations are the high costs in opposition to the relatively cheaper alternative of growing medicinal plants conventionally. This paper aims to quickly review the general origin of plant secondary metabolites, the leading techniques and recent advances for PDMC in vitro production, and the challenges around the use of this promising technology.

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Section: Main Factors Affecting Pdmc Productionmentioning

confidence: 99%

Section: Main Factors Affecting Pdmc Productionmentioning

confidence: 99%

Section: Main Factors Affecting Pdmc Productionmentioning

confidence: 99%

Section: Callus and Cell Suspension Culturementioning

confidence: 99%

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Advances and challenges on the in vitro production of secondary metabolites from medicinal plants

2019

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“…The primary and species-speci c secondary metabolites ingredients may be another factor of concern. For instance, the BL signi cantly increased the concentration of phenolic compounds in pea sprouts and basil [23,24], avonoids production in Cyclocarya paliurus [25], and the amount of salidroside in callus culture of Rhodiola imbricata [26]. By contrast, the red and far-red parts of the light spectrum induce the production of salicylic acid, which in turn increased avonoid accumulation in Ginkgo biloba [27].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and mebabolomic profiling: the effect of LED light quality on morphological traits, and phenylpropanoid-derived compounds accumulation in Sarcandra glabra seedlings

Xie

Chen

Zhou

et al. 2020

Preprint

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Background Sarcandra glabra is an evergreen and traditional Chinese herb, having medicinal significance as anti-oxidant, anti-bacterial, anti-inflammatory, and anti-tumor. Recently, China has initiated to establish cultivation of this plant in greenhouse under artificial light-emitting diodes (LED). However, little is known regarding the effects of the different LED lights on plant growth, accumulation pattern of secondary metabolites, and the molecular mechanism of Sarcandra glabra. Results Compared to white light (WL), the red light (RL) increased the height and decreased the stem diameter and leaf area, while blue light (BL) suppressed the height and leaf area. According to our transcriptome profiling, some differentially expressed genes (DEGs) were enriched in the phenylpropanoid biosynthesis. We identified 46 unigenes encoding for almost all known enzymes involved in phenylpropanoid biosynthesis, while the expression level of RNA-seq and qPCR were largely consistent. Meanwhile, we found 53 unigenes encoding R2R3-MYB proteins and 53 unigenes encoding bHLH proteins that several of them were related to flavonoids biosynthesis. Based on metabolomic profiling, a total of 454 metabolites were detected and the distribution of chemicals varied significantly. While flavonoids, phenolic acids, and tannins were mainly located in leaves; Organic acids, lignans and coumarins, and terpenoids were much more abundant in WG (root tissue under WL). Meanwhile, the yields of most flavonoids from BY (leaf tissue under BL) and the synthesis of primarily targeted compounds was lower than in WY (leaf tissue under WL) and RY (leaf tissue under RL). Instead, the leaves grown under RL exhibited a greater production of bioactive phytochemicals such as esculetin, fraxetin, esculin, and scopoletin. Conclusion These results provide further insight into the molecular mechanism of metabolites accumulation patterns in S. glabra under different light conditions, enabling the development of optimum breeding conditions for this plant.

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Plant Tissue Culture and Crop Improvement

Aslam,

Bibi,

Bibi

et al. 2023

Climate-Resilient Agriculture, Vol 2

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Influence of light quality on growth, secondary metabolites production and antioxidant activity in callus culture of Rhodiola imbricata Edgew

Cited by 101 publications

References 66 publications

Advances and challenges on the in vitro production of secondary metabolites from medicinal plants

Advances and challenges on the in vitro production of secondary metabolites from medicinal plants

Transcriptome and mebabolomic profiling: the effect of LED light quality on morphological traits, and phenylpropanoid-derived compounds accumulation in Sarcandra glabra seedlings

Plant Tissue Culture and Crop Improvement

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