Influence of light quality and some growth regulators in inducing the production of Podophyllotoxin, a bioactive compound against cancer, in adventitious roots formed in the leaves of Hyptis suaveolens

Silva, Sâmia Torres da; Coelho, Adriane Duarte; Assis, Rafael Marlon Alves de; Carvalho, Alexandre Alves de; Bertolucci, Suzan Kelly Vilela; Pinto, José Eduardo Brasil Pereira

doi:10.1016/j.indcrop.2022.115710

Cited by 2 publications

(3 citation statements)

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“…In the present study, in the absence of growth regulators or in the presence of different concentrations of IBA and NAA, contents ranging from 54.44 to 211.66 µg g -1 were observed (0.05 to 0.21%). Adventitious roots were induced in leaf explants and nodal segments of H. suaveolens and NAA was the best PGR to induce adventitious roots' emergence in leaves, whereas IBA was the best one for in nodal segment (Silva et al 2022). However, PGR NAA inhibited the synthesis of PTOX, but IBA induced this secondary compound (Silva et al 2022).…”

Section: Robustnessmentioning

confidence: 94%

“…Adventitious roots were induced in leaf explants and nodal segments of H. suaveolens and NAA was the best PGR to induce adventitious roots' emergence in leaves, whereas IBA was the best one for in nodal segment (Silva et al 2022). However, PGR NAA inhibited the synthesis of PTOX, but IBA induced this secondary compound (Silva et al 2022). The induction of adventitious roots in Podophyllum peltatum was optimal on 1/2 MS medium lacking NH4NO3 and supplemented with 14.78 μM IBA, also was observed PTOX production (Anbazhagan et al 2008).…”

Section: Robustnessmentioning

confidence: 99%

“…However, supplementation with 1 mg/mL naphthaleneacetic acid (NAA) negatively influenced PTOX production. Silva et al (2022) reported that adventitious root formation and PTOX induction in H. suaveolens leaves grown in semi-solid MS medium only happen in plants grown in the dark and under monochromatic red LED light.…”

Section: Introductionmentioning

confidence: 99%

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Accumulation of podophyllotoxin in root culture of Hyptis suaveolens (L.) Poit: a potential natural lignan for clinically useful anticancer drugs

Silva

Assis

Coelho

et al. 2023

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Podophyllotoxin (PTOX) is a natural antiviral, antirheumatic and anticancer molecule but its chemical synthesis is expensive. The present study aimed to develop and validate an analytical method by HPLC for the quantification of PTOX in roots of Hyptis suaveolens, as well as to evaluate the culture of its roots in vitro in liquid medium supplement with different concentrations of Indole-3-butyric acid (IBA), 1-Naphthaleneacetic acid (NAA), vitamins and myo-inositol. The analytical method was developed and validated. Root culture was used for biomass production and PTOX content was quantified using the developed analytical method after successful validation. The parameters that confirmed the analytical method were selectivity (peak purity: > 99%), system suitability (Rs = 2.92; N = 7064; k = 1.23; As = 1.31; DPR = 0.61%), intra-day and inter-day precision (DPR = 2.43% and 2.96%, respectively), linearity (R² = 0.997), recovery percentage (90.47–101.85%), limit of quantification (5.25 ng) and limit of detection (0.5 ng). Root culture in MS medium containing 1 mg L-1 IBA + 0.5 mg L-1 NAA showed the highest root dry weight (248.76 mg) and the highest PTOX concentration in the root (179, 97 µg-1). The vitamins and myo-inositol in the medium produced 198.88 mg of root dry weight and 6.01 µg g-1 of PTOX. The roots cultured in liquid MS medium supplemented with 1 mg L-1 of IBA + 0.5 mg L-1 of NAA maximized the root biomass and PTOX content. The adequate balance of vitamin and myo-inositol supplementation in liquid MS culture medium increased the production of root dry weight and PTOX accumulation.

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Section: Robustnessmentioning

confidence: 94%