Influence of light on cyclic nucleotide metabolism in plants; effect of dibutyryl cyclic nucleotides on chloroplast components

Brown, Eric G.; Newton, Russell P.; Evans, D. E.; Walton, Terence J.; Younis, Layla M.; Vaughan, Judith

doi:10.1016/s0031-9422(00)98040-3

Cited by 31 publications

(22 citation statements)

References 19 publications

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“…Our data showing the involvement of cGMP in GA signaling in the aleurone cell are consistent with other reports of the occurrence of cGMP in plant cells (Janistyn, 1983;Newton et al, 1991;Newton and Brown, 1992) and its role in ion channel regulation (Hiroshi, 1995) and phytochrome signaling pathways (Bowler et al, 1994). cGMP has been unambiguosly identified by mass spectrometry in a wide variety of plant tissues, including cereals (Janistyn, 1983); moreover, environmental signals such as light (Brown et al, 1989) and nitric oxide (Pfeiffer et al, 1994) cause dramatic increases in the levels of cGMP in plants. In bean, for example, exposure of plants to 1 hr of white light after a 12-hr dark period caused cGMP levels to increase fourfold (Brown et al, 1989).…”

Section: Discussionsupporting

confidence: 92%

“…cGMP has been unambiguosly identified by mass spectrometry in a wide variety of plant tissues, including cereals (Janistyn, 1983); moreover, environmental signals such as light (Brown et al, 1989) and nitric oxide (Pfeiffer et al, 1994) cause dramatic increases in the levels of cGMP in plants. In bean, for example, exposure of plants to 1 hr of white light after a 12-hr dark period caused cGMP levels to increase fourfold (Brown et al, 1989). The lightinduced increase in cGMP was transient, because cGMP returned to dark levels after 5 hr in light.…”

Section: Discussionmentioning

confidence: 99%

“…The lightinduced increase in cGMP was transient, because cGMP returned to dark levels after 5 hr in light. It is of interest that light-induced changes in the level of cGMP in bean plants were not accompanied by marked changes in the activities of either guanylyl cyclase or cGMP phosphodiesterase (Brown et al, 1989). Although it is not clear how light brings about changes in cGMP in bean plants, we infer that in aleurone tissue, the GA-induced rise in cGMP is via an effect on guanylyl cyclase, because LY, an inhibitor of guanylyl cyclase, prevents the increase in cGMP in response to GA.…”

Section: Discussionmentioning

confidence: 99%

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cGMP Is Required for Gibberellic Acid-Induced Gene Expression in Barley Aleurone.

et al. 1996

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The occurrence and roles of cGMP were investigated in aleurone layers and protoplasts isolated from barley (cv Himalaya) grain. Levels of cGMP in freshly isolated barley aleurone layers ranged from 0.065 to 0.08 pmol/g fresh weight of tissue, and cGMP levels increased transiently after incubation in gibberellic acid (GA). Abscisic acid (ABA) did not increase cGMP levels in aleurone layers. LY 83583 (LY), an inhibitor of guanylyl cyclase, prevented the GA-induced increase in cGMP and inhibited GA-induced a-amylase synthesis and secretion. The inhibitory effectsof LY could be overcome by membranepermeant analogs of cGMP. LY also prevented GA-induced accumulation of a-amylase and GAMYB mRNAs. cGMP alone was not sufficient to induce the accumulation of a-amylase or GAMYB mRNA. LY had a less dramatic effect on the accumulation of mRNAs encoding the ABA-responsive gene Rab21. We conclude that cGMP plays an important role in GA, but not ABA, signaling in the barley aleurone cell.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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cGMP Is Required for Gibberellic Acid-Induced Gene Expression in Barley Aleurone.

et al. 1996

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“…Therefore, we speculate that ABA (and PKABA1) blocks the GA signaling cascade downstream of the point of action of cGMP. Guanylyl cyclases appear to be a central component in other plant signal transduction networks, including light detection (Brown et al, 1989) and phytochrome A responses (Bowler and Chua, 1994). However, the molecular mechanism of cGMP action in plants is not yet well understood.…”

Section: Discussionmentioning

confidence: 99%

Gibberellin/Abscisic Acid Antagonism in Barley Aleurone Cells: Site of Action of the Protein Kinase PKABA1 in Relation to Gibberellin Signaling Molecules

Gómez-Cádenas¹,

Zentella²,

Walker-Simmons

et al. 2001

Plant Cell

255

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The antagonism between gibberellins (GA) and abscisic acid (ABA) is an important factor regulating the developmental transition from embryogenesis to seed germination. In barley aleurone layers, the expression of genes encoding ␣ -amylases and proteases is induced by GA but suppressed by ABA. It has been shown that an ABA-induced protein kinase, PKABA1, mediates the ABA suppression of ␣ -amylase expression. Using a barley aleurone transient expression system, we have now localized the site of action of PKABA1 relative to other signal transduction components governing the expression of ␣ -amylase . The expression of ␣ -amylase can be transactivated by the transcription factor GAMyb, which is itself induced by GA. A truncated GAMyb containing the DNA binding domain but lacking the transactivation domain prevents the GA induction of ␣ -amylase , further supporting the notion that GAMyb mediates the GA induction of ␣ -amylase expression. Although ABA and PKABA1 strongly inhibit the GA induction of ␣ -amylase , they have no effect on GAMyb-transactivated ␣ -amylase expression. Using a GAMyb promoter-␤ -glucuronidase construct, we also show that both ABA and PKABA1 repress the GA induction of GAMyb . In the slender mutant, GAMyb and ␣ -amylase are highly expressed, even in the absence of GA. However, this constitutive expression can still be inhibited by ABA, PKABA1, or an inhibitor of cGMP synthesis. On the basis of these observations, we suggest that PKABA1 acts upstream from the formation of functional GAMyb but downstream from the site of action of the Slender gene product. Because PKABA1 inhibits the GA induction of the GAMyb promoter-␤ -glucuronidase construct, it appears that at least part of the action of PKABA1 is to downregulate GAMyb at the transcriptional level. INTRODUCTIONThe interaction between phytohormones, particularly that between gibberellin (GA) and abscisic acid (ABA), is an important factor controlling the transition from embryogenesis to germination in seed. During germination of cereal grains, the embryo secretes GA to the aleurone layer, where it promotes the expression of several genes encoding hydrolytic enzymes (Ritchie and Gilroy, 1998b;Lovegrove and Hooley, 2000, and references therein). Expression of these genes is blocked by ABA during seed development, in dormant seeds, and in seedlings under unfavorable germination conditions. Cereal aleurone layers, therefore, are an excellent system in which to explore the molecular mechanisms involved in hormonally regulated gene expression, particularly the antagonism between GA and ABA (Bethke et al., 1997;Lovegrove and Hooley, 2000).The promoter sequences important for the GA induction and ABA suppression of ␣ -amylase genes have been studied extensively (Skriver et al., 1991;Gubler and Jacobsen, 1992;Lanahan et al., 1992;Rogers and Rogers, 1992). Three regions-box1 (amylase box), GARE (for gibberellin response element), and pyrimidine box-are found in the promoters of all GA-inducible ␣ -amylase genes. An additional region important for GA ind...

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“…Concentrations of 36 pmol cAMP/g fresh weight in Torenia stem segments (Ishioka and Tanimoto, 1990) and 5 pmol cAMP/g fresh weight in suspensioncultured cells of Pkaseolus vulgaris (Bolwell, 1992) have been described. Gangwani et al (1991) Brown and Newton, 1981;Brown et al, 1989), an effect that has been attributed in part to an increase in the K , of cAMP phosphodiesterase (Brown et al, 1989).…”

Section: S Camp Present In Hicher Plants?mentioning

confidence: 99%

Cyclic AMP as a Second Messenger in Higher Plants (Status and Future Prospects)

Assmann¹

1995

Plant Physiol.

103

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Adenosine 3':5'-monophosphate, commonly known as cAMP, is a key second messenger in living organisms ranging from Dictyostelium to Homo sapiens. cAMP was first described as a regulator of glycogen breakdown in the liver and is now known to be a second messenger for a wide variety of cellular responses in animals. cAMP is produced from ATP by the action of adenylate cyclase and is hydrolyzed to AMP by the enzyme cAMP phosphodiesterase.The question of whether cAMP is an authentic second messenger in higher plants has engaged plant scientists for decades. This article will highlight aspects of the controversies surrounding cAMP and suggest steps toward their possible resolution. Literature citations are largely restricted to the last 5 years, since earlier work has been summarized in severa1 reviews (

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Influence of light on cyclic nucleotide metabolism in plants; effect of dibutyryl cyclic nucleotides on chloroplast components

Cited by 31 publications

References 19 publications

cGMP Is Required for Gibberellic Acid-Induced Gene Expression in Barley Aleurone.

cGMP Is Required for Gibberellic Acid-Induced Gene Expression in Barley Aleurone.

Gibberellin/Abscisic Acid Antagonism in Barley Aleurone Cells: Site of Action of the Protein Kinase PKABA1 in Relation to Gibberellin Signaling Molecules

Cyclic AMP as a Second Messenger in Higher Plants (Status and Future Prospects)

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