Influence of Laser Phototherapy (λ660 nm) on the Outcome of Oral Chemical Carcinogenesis on the Hamster Cheek Pouch Model: Histological Study

Monteiro, Juliana Santos de Carvalho; Pinheiro, António Luiz Barbosa; Oliveira, Susana C. P. S. de; Aciole, Gilberth Tadeu dos Santos; Sousa, José Augusto Cardoso; Cangussú, Maria Cristina Teixeira; Santos, Jean Nunes dos

doi:10.1089/pho.2010.2896

Cited by 39 publications

(23 citation statements)

References 28 publications

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“…These results are in line with previous performed studies. The study of de C. Monteiro et al (21) showed in vivo progression after LLLT (56.4 J/cm2) of chemically induced squamous cell carcinoma, in the oral cavity of hamsters. Gomes Henriques et al reported increased proliferation and invasion properties of SCC cells irradiated with LLLT at low energy densities (0.5 and 1 J/cm2) in a dose and time-dependent manner (17).…”

Section: Discussionmentioning

confidence: 99%

Low-Level Laser Therapy Stimulates Proliferation in Head and Neck Squamous Cell Carcinoma Cells

2018

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Objectives: Low-level laser therapy (LLLT) is a promising non-invasive treatment option for oropharyngeal mucositis, which is a common side effect of many oncological treatments. LLLT is known for its wound healing properties due to the stimulation of cellular processes, such as proliferation, migration and differentiation. Controversy exists on the possible stimulatory effect of LLLT on head and neck cancer (HNSCC) cells in patients treated with radiotherapy. The aim of this study was to evaluate the biostimulatory effect together with the underlying mechanisms of LLLT on HNSCC cancer cells and normal epithelial cells.Materials and methods: HNSCC cell lines (SCC154, SQD9, and SCC61) and human tonsil epithelial cells were exposed to a Gallium-Aluminum-Arsenide diode laser (830 nm, 150 mW) with energy densities of 0, 1, and 2 J/cm2. The proliferation potential of the cells was assessed by Sulforhodamine B assay, immunoblotting (mitogenic pathways), immunocytochemistry (Ki67), and flow cytometry (PI cell cycle staining).Results: Cell proliferation was increased in HNSCC cell lines after laser irradiation with 1 J/cm2, whereas no significant increase was seen after laser irradiation with 2 J/cm2. In contrast, no effect on cell proliferation was seen in the human tonsil epithelial cells after laser irradiation with any of the energy densities. The increased proliferation was associated with elevated levels of pAKT, pERK, and Ki67 protein expression and cell cycle progression.Conclusion: Our results show that LLLT increases cell proliferation in a dose-dependent manner in HNSCC cells but not in normal epithelial tonsil cells. These results suggest that LLLT has to be used with caution when treating oropharyngeal mucositis in HNSCC patients since tumor cells present in the LLLT irradiation field could be triggered by LLLT.

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Section: Discussionmentioning

confidence: 99%

Low-Level Laser Therapy Stimulates Proliferation in Head and Neck Squamous Cell Carcinoma Cells

2018

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“…19 In the present study, the sessions took place twice a week for 6 weeks, with the objective of keeping a steady cell response and maintaining the patients' adherence to therapy. Furthermore, as some authors mention that LLLT can stimulate not only the healthy cells but also the tumor cells, [41][42][43] during LLLT applications, the areas close to the already-treated tumor lesions were avoided.…”

Section: Discussionmentioning

confidence: 99%

Effect of Low-Level Laser Therapy on Radiotherapy-Induced Hyposalivation and Xerostomia: A Pilot Study

Saleh

Figueiredo

Cherubini

et al. 2014

Photomedicine and Laser Surgery

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“…LLLT is considered to be a non-ionizing irradiation that is not able to induce damage to cells’ DNA; however, it can modify cell behavior [23, 28, 29], and that is why LLLT is generally counter-indicated for proliferating or dysplastic lesions that can potentially turn into neoplasia [19, 30]. Besides, in vitro cultured cancer cells may not represent the complex biological environment found in a tumor growing in its host [31, 32]; however, cell culture is the safest and simplest way of searching for possible cell growth and increased invasion produced by the modulation of specific signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

Low‐level laser therapy can produce increased aggressiveness of dysplastic and oral cancer cell lines by modulation of Akt/mTOR signaling pathway

Sperandio

Giudice

Corrêa

et al. 2013

Journal of Biophotonics

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Low-level laser therapy (LLLT) is a non-thermal phototherapy used in several medical applications, including wound healing, reduction of pain and amelioration of oral mucositis. Nevertheless, the effects of LLLT upon cancer or dysplastic cells have been so far poorly studied. Head and neck cancer patients receiving LLLT for oral mucositis, for example, might have remaining tumor cells that could be stimulated by LLLT. This study demonstrated that LLLT (GaAlAs – 660 nm or 780 nm, 40 mW, 2.05, 3.07 or 6.15 J/cm2) can modify oral dysplastic cells (DOK) and oral cancer cells (SCC9 and SCC25) growth by modulating the Akt/mTOR/CyclinD1 signaling pathway; LLLT significantly modified the expression of proteins related to progression and invasion in all the cell lines, and could aggravate oral cancer cellular behavior, increasing the expression of pAkt, pS6 and Cyclin D1 proteins and producing an aggressive Hsp90 isoform. Apoptosis was detected for SCC25 and was related to pAkt levels. Translocation of pAkt seen through Immunofluorescence from cytoplasm of control SCC9 cells (A) to nuclei of red (D) and infrared (G) laser irradiated cells. B, E and H: pAkt staining in control, red and infrared laser groups, respectively. C, F and I: nuclear DAPI in control, red and infrared groups, respectively.

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Influence of Laser Phototherapy (λ660 nm) on the Outcome of Oral Chemical Carcinogenesis on the Hamster Cheek Pouch Model: Histological Study

Abstract: It is concluded that LLLT, within the parameters used in the present study, caused a significant progression of the severity of SCC in the oral cavity of hamsters.

Cited by 39 publications

References 28 publications

Low-Level Laser Therapy Stimulates Proliferation in Head and Neck Squamous Cell Carcinoma Cells

Low-Level Laser Therapy Stimulates Proliferation in Head and Neck Squamous Cell Carcinoma Cells

Effect of Low-Level Laser Therapy on Radiotherapy-Induced Hyposalivation and Xerostomia: A Pilot Study

Low‐level laser therapy can produce increased aggressiveness of dysplastic and oral cancer cell lines by modulation of Akt/mTOR signaling pathway

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