Influence of laser photobiomodulation upon connective tissue remodeling during wound healing

Medrado, Alena Ribeiro Alves Peixoto; Soares, Ana Prates; Santos, Elisângela Trindade; Reis, Sílvia Regina de Almeida; Andrade, Zilton A.

doi:10.1016/j.jphotobiol.2008.05.008

Cited by 88 publications

(62 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…On the other hand, in the later stages of the repair process the fibers exhibit an organizational pattern more compact, thicker bundles of red-orange color, indicating a type I collagen 2,11,19 .…”

Section: Discussionmentioning

confidence: 99%

“…Several studies on laser photobiomodulation ensured the improvement of cicatricial repair in wounds with loss of tissue, a particular sort of cicatrization phenomenon known as second-intention wound healing 2,7,11 . However, in this study, the experimental lesions were sutured wounds, resembling the ones carried out after elective surgical procedures, in order to asses the role of LLLT as an alternative therapy to improve the healing process in cases in which the cicatrization might be somehow impaired.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, it seems that despite laser arrays is able to upregulate the activity of enzymes responsible for the synthesis of collagen fibers 2,7 , this activity, in sutured wounds, seems not to be expressive enough to lead to an overproduction of collagen fibers. These data appears to suggest that the upregulatory effects of LLLT on collagenization, particularly observed in secondintention wound healing 2,9,11 , do not enhance the possibility of hypertrophic scars formation in sutured wounds.…”

Section: Discussionmentioning

confidence: 99%

“…In this regard, numerous chemicals are released into the injured area causing tissue changes consistent with an acute inflammatory process. This phenomenon is characterized by stimulation, migration and proliferation of stromal cells, synthesis of extracellular matrix, and finally culminating in tissue remodeling [1][2][3] .…”

Section: Introductionmentioning

confidence: 99%

“…Currently, there are methods to alleviate the inflammatory process, providing the improvement of cellular nutrition and favoring the injured tissue repair, particularly the low level laser therapy (LLLT) 2,4 . The LLLT has been clinically used for pain control and wound healing.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Effect of low level laser on sutured wound healing in rats

et al. 2011

View full text Add to dashboard Cite

Purpose:To evaluate the effect of low-level laser therapy (LLLT) ë904 nm on healing of surgical wounds in rats. Methods: Forty male Wistar rats were used, divided into four groups, underwent incision along the lines Alba covering skin, subcutaneous and muscle abdominal, sutured continuously for nylon 5-0. Eight and fifteen days after the surgery process, the repairing area was removed and histological sections were stained with hematoxylin-eosin to assess cellularity inflammatory, Masson´s Trichrome and Picrossirus to quantify the collagen fibers and immunohistochemical technique for counting newly formed vessels. The data were compared statistically using analysis of variance ANOVA, with a "post-hoc Tukey test, p <0.05. Results: Low-level laser therapy reduced the intensity of the inflammatory reaction and influenced the dynamic of the immunoinflammatory response by inducing switching of the leukocyte infiltration pattern (neutrophilic to lymphoplasmacytic infiltration). Also stimulate the deposition and enhance the organization of collagen fibers, featuring a delicate collagen type III. Furthermore, it appeared to a significant increase in the average number of newly formed vessels (p = 0.00 and p = 0.02, respectively). Conclusion: Low-level laser therapy resulted in modulate of the inflammatory response, enhanced deposition of collagen fibers and increase in the average number of newly formed vessels. Key words: Wound Healing. Lasers, Semiconductor. Collagen. Macrophages. Rats. RESUMO Objetivo:Avaliar o efeito da terapia laser de baixa potência (TLBP) ë904 nm na cicatrização de feridas cirúrgicas em ratos. Métodos: Foram utilizados 40 ratos Wistar, machos, divididos em quatro grupos, submetidos à incisão na linha Alba abrangendo pele, subcutâneo e musculatura abdominal, suturados continuamente por fios de nylon 5-0. Oito e quinze dias após o procedimento cirúrgico, a área de reparo foi removida e os cortes histológicos foram corados com hematoxilina-eosina para avaliar celularidade do infiltrado inflamatório, Tricrômico de Masson e Picrosirius para quantificar as fibras de colágeno e técnica imuno-histoquímica para a contagem de vasos neoformados. Os dados foram comparados estatisticamente pela análise de variância ANOVA, com "teste de Tukey post-hoc, p <0,05. Resultados: A terapia laser de baixa potência reduziu a intensidade da reação inflamatória e influenciou a dinâmica da resposta imuno por induzir a mudança do padrão de infiltração de leucócitos (neutrófilos a infiltração mononuclear). Como também estimula a deposição e melhora padrão organizacional das fibras colágenas, apresentando um delicado colágeno tipo III. Além disso, verificou-se um aumento significativo no número médio de vasos neoformados (p = 0,00 e p = 0,02, respectivamente). Conclusão: A terapia laser de baixa potência modula a resposta inflamatória, com melhor deposição de fibras colágenas e aumento do número médio de vasos neoformados. Descritores: Cicatrização de Feridas. Lasers Semicondutores. Colágeno. Macrófagos. Ratos. -Act...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations