With femtosecond pulse excitation at the wavelength near 800nm, we observed three upconverted emission peaks from ZnO nanowires. The ultraviolet peak at about 385nm and the green emission band centered at about 515nm were attributed to the near band edge emission and defect level emission, respectively, while the intermediate peak was assigned to the second harmonic generation. From the quadratic dependence of the emission intensity on the excitation pulse energy, it is confirmed that the 385nm photoluminescence was mainly generated via two-photon absorption in ZnO nanowires under very intense light-matter interaction.