Influence of laser and LED irradiation on mast cells of cutaneous wounds of rats with iron deficiency anemia

Abstract

This work aimed to study histologically the effect of Laser or LED phototherapy on mast cells on cutaneous wounds of rats with iron deficiency. 18 rats were used and fed with special peleted iron-free diet. An excisional wound was created on the dorsum of each animal which were divided into: Group I -Control with anemia + no treatment; Group IIAnemia + Laser; Group III -Anemia + LED; Group IV -Healthy + no treatment; Group V -Healthy + Laser; Group VI -Healthy + LED. Irradiation was performed using a diode Laser (λ660nm, 40mW, CW, total dose of 10J/cm 2 , 4X2.5J/cm 2 ) or a RED-LED ( λ700nm, 15mW, CW, total dose of 10J/cm 2 ). Histological specimens were routinely processed, cut and stained with toluidine blue and mast cell counts performed. No significant statistic difference was found between groups as to the number of degranulated, non-degradulated or total mast cells. Greater mean values were found for degranulated mast cells in the Anemia + LED. LED irradiation on healthy specimens resulted in a smaller number of degranulated mast cells. Our results leads to conclude that there are no significant differences in the number of mast cells seven days after irradiation following Laser or LED phototherapy.