Influence of laser ablation parameters on trueness of imaging

Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration

“…This optimization was performed by ablation of white paper with printed ink lines of 800 μm thickness. For this purpose, the 63 Cu signal was recorded because this element is present in the used ink10.…”

“…In imaging by means of LA-ICP-MS, a broadening of imaged patterns occurs that can be evaluated as described previously101112. The broadening is mainly due to a combination of the laser spot size and scan speed rate.…”

“…Due to the large dimensions of the imaged tissue samples (approximately 8 × 5 mm), a laser spot size of 100 μm was selected to reach a minimal LOD. An increase in the laser spot size resulted in a lower LOD1013. The apparent width w app was calculated to evaluate the broadening caused by the various scan speed rates.…”

Influence Of Spot Size On LA-ICP-MS Ablation Behavior For Synthetic Calcium Tungstate And Silicate Glass Reference Material NIST SRM 610

“…NIST SRM 610). [18][19][20][21][22] Scheelite is a common accessory mineral that has the chemical composition of CaWO4. It forms in various types of hydrothermal deposits and always incorporates abundant trace elements, such as the rare earth elements (REEs) of Nb, Pb and Mo, via substitution for Ca 2+ or W 6+ in the crystal lattice, which renders scheelite to exhibit distinct trace element geochemistry and thus serves as a fingerprint for deposit types, metallogenic settings and oreforming fluid compositions.…”

Quantitative metrics for assessment of chemical image quality and spatial resolution

“…Recently, Vaculovic et al ., using well‐defined printed inked lines on paper, studied the influence of laser spot size and scan speed on lateral resolution in laser ablation inductively coupled plasma (LA‐ICP)‐MS imaging. In their work they made use of a metric termed "trueness of imaging", defined as the relative broadening ( Δw rel ) of a printed line with theoretical and experimentally observed widths of w and w app , respectively, as Δw rel (%) = 100( w app – w )/ w .…”