Influence of laminin substratum on cell proliferation and CALC I gene expression in medullary thyroid carcinoma C cell lines

Lekmine, Fatima; Lausson, S.; Pidoux, E.; Segond, N.; Roos, Bernard A.; Treilhou-Lahille, F.; Jeanne, N.

doi:10.1016/s0303-7207(99)00138-0

Cited by 8 publications

(7 citation statements)

References 35 publications

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“…Fibronectin, in particular, is known to help cells attach to ECM 4, 31. The inhibition of cell adhesion and proliferation by physiosorption of laminin was in accordance with previous studies which showed that laminin also decreased the proliferation rate of the medullary thyroid carcinoma CA‐77 cell line 32. Laminin and fibronectin differ in the ability to enhance cell adhesion.…”

Section: Resultssupporting

confidence: 90%

“…4,31 The inhibition of cell adhesion and proliferation by physiosorption of laminin was in accordance with previous studies which showed that laminin also decreased the proliferation rate of the medullary thyroid carcinoma CA-77 cell line. 32 Laminin and fibronectin differ in the ability to enhance cell adhesion. It has been proposed that fibronectin is relatively nonspecific in promoting cell adhesion, while laminin shows more restricted specificity towards cell type.…”

Section: Caco-2 Attachment On Pdms Surfaces Coated With Extracellularmentioning

confidence: 99%

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Chemical and physical modifications to poly(dimethylsiloxane) surfaces affect adhesion of Caco‐2 cells

Wang

Sun

Ziemer

et al. 2009

J Biomedical Materials Res

122

106

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Polydimethylsiloxane (PDMS) silicone elastomer is extensively used in soft lithography processes to fabricate microscale or nano scale systems for microfluidic or cell culture applications. Though PDMS is biocompatible, it is not an ideal material for cell culture due to its poor cell adhesion properties. In this study, PDMS surfaces were modified to promote intestinal cell adhesion, in the interest of testing feasibility of using microfabricated PDMS systems for high throughput drug screening. Modification techniques included changing chemical composition of PDMS (i.e., varying curing to mixing agent ratio, and oxidization of PDMS surface by oxygen plasma), surface treatment of PDMS by coating with charged molecules (i.e., poly-D-lysine, L-alpha-phosphatidylcholine, and a layer bylayer coating), and deposition of extracellular matrix (ECM) proteins (i.e., laminin, fibronectin, and collagen). The influence of these modifications on PDMS properties, including elastic modulus and surface properties (wettability, chemical composition, topography, and protein adsorption) were characterized. Modification techniques were all found to change PDMS properties and influence the attachment and proliferation of Caco-2 cells over three days of culture to varying degrees. Generally, Caco-2 cells preferred to attach on collagen-coated, fibronectin-coated, and fibronectin-coated oxygen-plasma treated PDMS. The results highlight the importance of considering multiple physical and chemical factors that may be influenced by biomaterial modification and result in altered cell attachment to microfabricated systems, including surface hydrophobicity, chemical composition, stiffness, and topography. This study provides a foundation for further miniaturization, utilizing soft lithography techniques, of Caco-2 cell-based system for high-throughput screening of drug intestinal absorption during lead optimization in drug discovery. The understanding of different surface modifications on adjusting cell adhesion on PDMS allows systemic design of Biomicroelectromechanical Systems (BioMEMS) with tunable cell adhesion properties.

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Section: Resultssupporting

confidence: 90%

Section: Caco-2 Attachment On Pdms Surfaces Coated With Extracellularmentioning

confidence: 99%

Chemical and physical modifications to poly(dimethylsiloxane) surfaces affect adhesion of Caco‐2 cells

Wang

Sun

Ziemer

et al. 2009

J Biomedical Materials Res

122

106

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“…Besides the hypocalcemic effect of CT, it is well established that CT administration shows regulatory effects on a variety of hormone secretion. Secretion of gastrin [6], insulin [3], GH [16], TSH [15], prolactin [20], and prostaglandin [4] were suppressed by the preinfusion of CT. On the other hand, hormone secretions were stimulated by CT infusion in the following hormones, such as ACTH [14], somatostatin [5,10,17], and beta-endorphin [14] secretions. Auto-regulation of CT secretion has been suggested as a feedback mechanism by some researchers using porcine thyroid slices [1,21].…”

Section: Introductionmentioning

confidence: 92%

Exogenous Calcitonin Suppresses Growth Fraction of Thyroid C Cells

Mori

Nakamura

Iteya

et al. 2003

Acta Histochem. Cytochem

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Calcitonin (CT) is a peptide hormone synthesized and secreted by the parafollicular (C) cells of the thyroid. Little is known about the mechanisms controlling proliferation of C cells by other humoral factors including CT, and there is no report that CT suppresses C cell proliferation. The effects of short-term administration of CT on C cell growth fraction were analyzed using BrdU and CT double immunohistochemical method in the rat thyroid. Continuous administration of 0.4 IU/kg and 40 IU/kg synthetic salmon CT for 14 days induced dosedependent suppression of BrdU labeling number of C cells without decrease of C cell to the total thyroid follicular cell ratio. These results suggest that exogenous CT may induce negative feedback to C cell proliferation in the thyroid.

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“…Laminin (about 850 KDa) is a component of the extracellular matrix and consists of various combinations of a long chain ␣ chain) and two shorter chains ␤ and ␥ linked together to form an elongated cruciform shape. [27][28][29][30] Laminin has binding sites for type IV collagen, heparin, and integrins on cell surfaces. 28 Collagen interacts with laminin, which, in turn, interacts with integrins or other laminin receptor proteins, thus anchoring the basal lamina to the cells.…”

Section: Discussionmentioning

confidence: 99%

In vitro release kinetics of proteins from bioactive foams

Lenza

Jones

Vasconcelos

et al. 2003

J Biomedical Materials Res

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This study describes an approach to obtaining 3-D scaffolds for tissue engineering that allows the incorporation and release of biologically active proteins to stimulate cell function. Laminin was adsorbed on the textured surfaces of binary 70S30C (70 mol % SiO(2), 30 mol % CaO) and ternary 58S (60 mol % SiO(2), 36 mol % CaO, 4 mol % P(2)O(5)) foams. The covalent bonds between the binding sites of the proteins and the ligands on the scaffolds' surfaces did not denaturate the proteins. In vitro studies show that the foams modified with chemical groups and coated with laminin were bioactive, as demonstrated by the formation of a crystalline hydroxy carbonate apatite (HCA) layer formed on the surfaces of the foams upon exposure to simulated body fluid (SBF). The release of proteins from the foams also was investigated. Sustained and controlled release from the scaffolds over a 30-day period was achieved. Laminin release from the bioactive foams followed the dissolution rate of the material network. These results suggest that bioactive foams have the potential to act as scaffolds for soft-tissue engineering with a controlled release of proteins that can induce tissue formation or regeneration.

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Influence of laminin substratum on cell proliferation and CALC I gene expression in medullary thyroid carcinoma C cell lines

Cited by 8 publications

References 35 publications

Chemical and physical modifications to poly(dimethylsiloxane) surfaces affect adhesion of Caco‐2 cells

Chemical and physical modifications to poly(dimethylsiloxane) surfaces affect adhesion of Caco‐2 cells

Exogenous Calcitonin Suppresses Growth Fraction of Thyroid C Cells

In vitro release kinetics of proteins from bioactive foams

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