Influence of Lactoproteinum Solution With Sorbitol on Dna Content of Cells of Endocrine Glands on the Background of Skin Burn in Rats

Dzevulska, І. V.; Kovalchuk, Oleksandr; Черкасов, Е. В.; Majewskyi, О. Ye.; Shevchuk, Yu. G.; Пастухова, В. А.; Kyselová, Tatiana

doi:10.26724/2079-8334-2018-2-64-33-39

Cited by 16 publications

(14 citation statements)

References 15 publications

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“…It is known that the mechanisms of adaptation to hypoxia are realized on the principle of stress reactions with the participation of the hypothalamic-pituitary-adrenal system, the least explored is the role of the hypothalamic-pituitarythyroid system [2,3,4,10,11,23]. In the scientific literature, there is virtually no data on the participation of hormones in adapting to high temperatures.…”

Section: Discussionmentioning

confidence: 99%

“…In the case of severe thermal trauma, there are a number of factors that lead to sepsis and multiple organ failure: microcirculation disorder, presence of necrotic tissues inhabited by wounds, endogenous intoxication, activation of lipid peroxidation, development of syndrome of systemic inflammatory response, DIC, etc. [2,3,5,6,7,10,13,14,20]. It has been established that in the complicated and inadequately studied pathogenesis of burn disease one of the main places has endogenous intoxication, which is the result of proteolysis of damaged tissues and alteration of histo-hematological barriers.…”

Section: Introductionmentioning

confidence: 99%

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Features of the thyroid gland structural components remodeling in the toxemia stage after experimental thermal injury

Koritskiy

Небесна

2018

Rep. of Morph.

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Thermal injury causes severe structural and metabolic disturbances not only of the skin itself, but also of all organs and systems of the affected organism, is a manifestation of a complex symptom complex - a burn disease. Of particular importance in patients with burns are changes in the endocrine system. The aim of the study was to establish the microscopic and electron-microscopic reorganization of the components of the thyroid gland of animals after thermal damage on 14 day after experimental thermal injury. A III degree burn was applied under ketamine anesthesia with copper plates heated in boiled water to a temperature of 97-100°C. The size of the lesion area was 18-20% of the epilated surface of the body of rats. An experimental study of the structural components of the thyroid gland after a burn injury was performed on laboratory white male rats weighing 160-180 g. Rats euthanasia was performed after ketamine anesthesia by decapitation. In the experiment, the study of the microscopic and submicroscopic state of the follicles and hemocapillaries of the thyroid gland after thermal injury of the III degree. It has been established that in the toxemia stage after the application of the burn injury on 14 day (late toxemia stage), significant destructive and degenerative changes are found in the thyrocytes of the wall of the follicles and hemocapillaries, the organ acquires a macrofollicular structure. The height of thyrocytes decreases, the nuclei and organelles of cytoplasm are significantly damaged, the number and height of microvilli on their apical surface decreases, which negatively affects the cell's secretory cycle and transcapillary organ metabolic processes. The established destructive changes in the blood capillaries and thyrocytes of the follicles are the morphological manifestation of the suppression of the secretory activity of the thyroid gland during thermal injury and corresponds to the hypofunctional state of the organ.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Features of the thyroid gland structural components remodeling in the toxemia stage after experimental thermal injury

Koritskiy

Небесна

2018

Rep. of Morph.

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“…Опікова травма є однією з широко розповсюджених травматичних пошкоджень і спостерігається у 5,6-12,0% від усіх потерпілих з іншими видами травматизму [3,4,6,11,14,18]. У відповідь на опікову травму в організмі розвивається велика кількість патологічних процесів, які охоплюють практично усі органи і системи, приводячи до вираженого порушення гомеостазу, зриву адаптаційних процесів [2,4,10,11,12,13,15,19]. На даний час зростає інтерес до вивчення механізмів пошкодження і структурної перебудови органів ендокринної системи, викликаної різними патологічними факторами.…”

Section: вступunclassified

“…На даний час зростає інтерес до вивчення механізмів пошкодження і структурної перебудови органів ендокринної системи, викликаної різними патологічними факторами. Ендокринна система, володіючи широким діапазоном гормональних впливів на різні органи та системи, відіграє першочергову роль у виникненні зворотних реакцій організму на дію надзвичайного подразника [2,4,10,16,17]. При важких термічних травмах, щитоподібна залоза в зв'язку з притаманними їй морфофункціональними особливостями є своєрідним органом-мішенню.…”

Section: вступunclassified

Peculiarities of structural reorganization of the thyroid gland vessels in dynamics after experimental thermal trauma

Korytskyi¹

2018

Rep. of Vinnytsia Nation. Med. Univ.

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Thermal trauma and burn disease, which develops from deep, large lesions, are accompanied by significant morphological and functional changes not only in the skin, but also in organs of all systems of the body, including thyroid gland. The purpose of the study was to establish a microscopic reorganization of the animal thyroid gland in dynamics after an experimental thermal trauma. Burns of the third degree were applied under ketamine anesthesia with copper plates heated in boiled water to a temperature of 97–100 °С. The affected area corresponded 18–20% of the surface of the rat’s body. Experimental study of structural changes in the vascular bed of the thyroid gland after severe thermal trauma was carried out on laboratory white male rats weighing 160–180 g. Euthanasia of rats was performed after ketamine anesthesia, by decapitation. Structural changes in the thyroid gland were studied after 1, 7, 14 and 21 days from the beginning of the experiment. Histologic sections of 5–6 µm thickness were stained with hematoxylin-eosin. For the study of the connective tissue condition and its main substance, silver impregnation was performed by using the Gordon-Sweet's method (to detect reticular and immature collagen fibers), staining with 3-colored MSB — selective coloring method for intravascular coagulation syndrome. It has been established that in the early stages after the thermal trauma (1–7 days of the experiment, the stage of shock and early toxemia) there are adaptive-compensatory changes and initial signs of destruction of the vascular bed structural components of the organ. In late terms (14–21 days of the experiment, the stages of late toxemia and septicotoxemia) significant destructive-degenerative changes of the thyroid gland arteries, veins and blood vessels of the hemocirculatory bed were detected. Therefore, a severe burn injury causes deep histological modification of the thyroid gland vessels, the degree of intensity of which depends on the time interval of the experiment, which adversely affects on the functioning of the organ and transvascular metabolic processes. In further researches it is planned to study the course of morphological changes of thyroid gland vessels during thermal trauma with combined application of corrective methods.

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“…Deep widespread burns are characterized not only by damage to the integumentary tissues, but also cause burn disease, which is characterized by different, lasting and peculiar general morphological and functional changes of all organs and systems of the organism [16]. It is clear that burn injury of the skin causes significant changes, first of all, skin cells [15], but damage to the skin is considered to be the main pathogenic mechanism that causes the development of burn disease, the components and factors of which are: generalized catabolic response in the trauma center and in all internal organs, systemic inflammatory and apoptotic responses, endogenous intoxication and multiple organ dysfunction [3,8,9].…”

Section: Introductionmentioning

confidence: 99%

Structural changes of duodenal mucosa enterocytes of rats in burn skin injury under experimental streptozotocin-induced diabetes mellitus

Cherkasov

Tymoshenko

2019

Rep. of Morph.

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The work is devoted to the study of structural changes of enterocytes in the mucous membrane of the duodenum in a burn injury of the skin of a rat under conditions of experimental streptozotocin induced diabetes. The study was carried out on laboratory white adult rats-males weighing 180-210 g. The control group consisted of 21 animals without somatic pathology, the first experimental group consisted of 21 rats with skin burn injury, the second experimental group cleared 21 rats with skin burn and experimental streptozotocin induced diabetes. A model of experimental diabetes mellitus was reproduced by administering streptozotocin intraperitoneally once in dose of 50 mg/kg to rats. In an experimental simulation of a skin burn, two copper plates in the form of an ellipse were kept in water at 100oC for 10 minutes and, under ether anesthesia conditions, were applied simultaneously symmetrically on both exposed parts of the body of rats with an exposure for 10 seconds. Burn skin damage in rats was II-AB degrees – dermal surface burn (according to the old classification III-A degree) with a total area of 21-23% of the body surface with the development of burn shock. For morphological studies, the duodenum was taken, fragments of which were processed using conventional light and electron microscopy. The main criteria for assessing damage the enterocytes of the duodenal mucosa were the results studies of histological and ultrastructural data over 7, 14 and 21 days after a skin burn. The results of the studies showed that the damage of the enterocytes of the duodenal mucosa is based on deep destructive changes, which after 21 days (at the stage of septic toxemia), as a rule, are not reversible and develop against the background of significant intoxication of the organism. In the mucous membrane of the duodenum with burn injury of the skin associated with diabetes mellitus, there is a deterioration of the manifestations of the adaptive response and prolongation of destructive processes, accompanied by a violation of intercellular interactions in cytoarchitectically modified and deformed villi and crypts.

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Influence of Lactoproteinum Solution With Sorbitol on Dna Content of Cells of Endocrine Glands on the Background of Skin Burn in Rats

Cited by 16 publications

References 15 publications

Features of the thyroid gland structural components remodeling in the toxemia stage after experimental thermal injury

Features of the thyroid gland structural components remodeling in the toxemia stage after experimental thermal injury

Peculiarities of structural reorganization of the thyroid gland vessels in dynamics after experimental thermal trauma

Structural changes of duodenal mucosa enterocytes of rats in burn skin injury under experimental streptozotocin-induced diabetes mellitus

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