Olive tree cultivation started in Syria million years ago and extended into the Mediterranean basin to become one of the basic constituents of the economies of most Mediterranean countries, which account for more than 90% of the world's olive oil production. In addition, the increasing international demand for olive oil in the last two decades has led to expansion of cultivation in places far from its origin, such as California, Argentina, and Australia. The fact that both production and consumption of olive oil are growing in the world is mainly due to its exceptional organoleptic characteristics, which are determined by the metabolites that it contains. Between them, the relative proportion of fatty acids is what greatly determine the oxidative stability and nutritional properties of the olive oil. Although cultivar is the main factor determining olive oil fatty acid composition, environmental factors, and culture conditions can also change the fatty acid profile. For that reason, understanding how these factors could affect the olive oil fatty acid composition is of special relevance, not only to increase its quality but also to establish the optimal growing conditions in new environments and to select new olive cultivars with improved quality characteristics. This article tries to summarise the present state of knowledge about the effect that different environmental stresses and culture conditions have on the olive fatty acid desaturation pathway. Special attention will be paid to the underlying molecular mechanisms by which olive fatty acid desaturases are regulated by temperature, light, nutrient availability and irrigation.