Influence of iron-limited and excess continuous culture on the virulence properties of Neisseria gonorrhoeae

Keevil, C. W.; Davies, D. B.; Spillane, B. J.; Mahenthiralingam, Eshwar

doi:10.1007/978-94-009-1383-7_114

Cited by 3 publications

(2 citation statements)

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“…Pan 1 and Pan 2 were highly conserved among gonococcal strains in that they had identical apparent molecular masses. During continuous culture, a protein with a molecular mass similar to that of Pan 1 was observed only under anaerobic conditions, whereas other proteins that were expressed under these conditions were also observed under other conditions of nutrient limitation (26). Thus, Pan 1 may be specifically induced by anaerobiosis, and Pan 2 and Pan 3 may be synthesized in response to nutritional stress.…”

Section: Sulfur Metabolismmentioning

confidence: 96%

“…The bacteria remained piliated for longer than 21 days and were virulent when evaluated in the guinea pig subcutaneous chamber model. Keevil et al (26,27) found that iron-limited gonococci remained piliated and were highly virulent for guinea pig subcutaneous chambers. However, switching to glucose-limited growth resulted in loss of piliation but retention of virulence.…”

Section: Continuous Culturementioning

confidence: 99%

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Physiology and metabolism of Neisseria gonorrhoeae and Neisseria meningitidis: implications for pathogenesis

et al. 1989

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Gonococci and meningococci are obligate human pathogens that can infect diverse sites within the human host. Each of these sites represents a unique niche with respect to nutrients, environmental factors, and competing microorganisms. The growth environment has a marked effect on the metabolism and cellular composition of Neisseria gonorrhoeae and N. meningitidis. Altered cellular composition is often reflected by changes in the cell surface that can ultimately affect the interaction of these microorganisms with the human host. The physiology and metabolism of the pathogenic Neisseria spp. have not been reviewed since 1979 (41). This review will discuss selected areas that have implications for the pathogenesis of these important microorganisms. IRON METABOLISMAfter entry into the human host, N. gonorrhoeae and N. meningitidis must multiply to colonize mucosal surfaces and to establish an infection. Cell growth and multiplication require essential nutrients such as iron. To obtain iron, the pathogenic Neisseria spp. must acquire it from the host. Despite the relative abundance of iron in the host, there is little free iron because of its sequestration by the ironbinding proteins transferrin (TF) and lactoferrin (LF) (19). In serum and interstitial fluid, iron is associated with TF; in breast milk, semen, and mucosal surfaces, it is associated primarily with LF. Consequently, gonococci and meningococci must possess mechanisms for utilizing the iron associated with the host iron-binding proteins as well as other potential in vivo iron sources such as heme and hemoglobin.Several studies have implicated iron in the virulence of the pathogenic Neisseria spp. Calver et al. (7) demonstrated that injection of ferrous sulfate prior to or injection of either iron sorbitol citrate or iron-dextran concomitantly with injection of N. meningitidis increased the lethality of several different meningococcal serogroups for mice by up to a 106-fold. The effect of the added iron was partially abrogated by the prior incubation of N. meningitidis with Desferal (CIBA-GEIGY Corp.), an iron chelator from which meningococci and gonococci are unable to remove iron (36). Holbein et al. (24) showed that the 50% lethal dose of N. meningitidis strains in a mouse model was decreased 109-fold by the concomitant administration of iron-dextran with the inoculum. Payne and Finkelstein (50) found that the intravenous inoculation of iron-containing compounds together with avirulent (nonpiliated) gonococci increased the lethality of these avirulent organisms for chicken embryos.Hafiz et al. (20) All strains of N. gonorrhoeae and N. meningitidis are able to grow with 25% iron-saturated TF as their sole source of iron (37). Archibald and DeVoe (1, 2) found that meningococci were capable of obtaining iron from a variety of iron-containing compounds including gastric mucin, ferric citrate, hemoglobin, myoglobin, and human TF. Iron complexed with a number of metabolic organic acids, polyphosphates, and several synthetic polycarboxylic acids was also re...

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Section: Sulfur Metabolismmentioning

confidence: 96%

Section: Continuous Culturementioning

confidence: 99%

Physiology and metabolism of Neisseria gonorrhoeae and Neisseria meningitidis: implications for pathogenesis

et al. 1989

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Meningoccal vaccines

Wall¹

1989

Recent Developments in Prophylactic Immunization

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Epitopes of serogroup BNeisseria meningitidisanalysed in vitro and directly from cerebrospinal fluid

Wall

Davies²,

Borriello

1989

FEMS Microbiology Letters

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Two type B15 P1.16 strains of Neisseria meningitidis were examined by immunogold electron microscopy for accessibility of two outer membrane protein (OMPs) to monoclonal antibodies. Both strains exhibited cell‐to‐cell variation of one epitope of the Class 3 OMP (P3.15) and one of the Class 1 OMPs (P1.16) when grown in vitro. One strain, a nasopharyngeal isolate revealed this variation to be growth‐phase independent and double labelling of both epitopes showed independent variation. CSF containing N. meningitidis was stored in liquid nitrogen without laboratory processing at the time of isolation of the second strain. Direct analysis of the organisms showed no cell‐to‐cell variation and immunoglobulin G on the surface. However, while there were similar labelling densities of the Class 1 epitope in vivo compared with either strain grown in vitro, there was a lower labelling density of the Class 3 epitope in vivo that was not caused by freeze‐thawing. This reduction may be due to decreased expression of this epitope in vivo which cast doubt on the use of the Class 2/3 OMP as a vaccine candidate.

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Influence of iron-limited and excess continuous culture on the virulence properties of Neisseria gonorrhoeae

Cited by 3 publications

References 3 publications

Physiology and metabolism of Neisseria gonorrhoeae and Neisseria meningitidis: implications for pathogenesis

Physiology and metabolism of Neisseria gonorrhoeae and Neisseria meningitidis: implications for pathogenesis

Meningoccal vaccines

Epitopes of serogroup BNeisseria meningitidisanalysed in vitro and directly from cerebrospinal fluid

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