Influence of intrauterine administration of Lactobacillus buchneri on reproductive performance and pro-inflammatory endometrial mRNA expression of cows with subclinical endometritis

Abstract

Potential beneficial effects of lactic acid bacteria on the genital health of cows become of particular interest when considering the importance of an optimal uterine health status for the success of breeding in dairy farming. Therefore, the aim of the present study was to analyse the influence of an intrauterine administration of the Lactobacillus buchneri DSM 32407 on reproductive performance, uterine health status, endometrial mRNA expression of pro-inflammatory factors of cows with signs of subclinical endometritis (SCE). L. buchneri DSM 32407 (n = 56; [LAC]) or a placebo (n = 60; [PLA]) was administered on day 24–30 postpartum. Endometrial cytobrush samples of cows with SCE were taken before the administration and at three following weeks (n = 16 cows each for LAC/SCE and PLA/SCE). A higher proportion of cows of the LAC and LAC/SCE group was pregnant after the first service and median days to conception for cows pregnant on day 200 pp were shorter. Three weeks after the administration, the endometrial mRNA expression of CXCL1/2, CXCL3, CXCR2, IL1B, IL8 and PTPRC was lower in the LAC/SCE group compared with the PLA/SCE group. These findings suggest that the presence of L. buchneri DSM 32407 contributes to a uterine environment that results in a better reproductive performance.

Intrauterine honey infusion in Holstein Frisian cows with purulent endometritis

“…32 As mentioned above, intrauterine alternative proved the in vitro activity against bovine uterine pathogens 3,8 were advised without in vivo studies, while others failed to improve the reproductive performance when tried in vivo. 6,28,33,34 Certain studies 10,11 on intrauterine infusion did not differ from antibiotic application, while some of them proved to be having in vivo activity when be used alone. 1,4,5 or in combination with others.…”

“…3 It is frequently treated with antibiotic intrauterine infusion, but with the emergence of MDR bacterial strains the alternative antimicrobial therapy for bovine metritis and endometritis is required. 3,4 Several therapeutic alternative agents proved in vitro antimicrobial activity were recommended to be tried as intrauterine infusion such as lactic acid, 1,4,5,6 herbal essential oil extracts (garlic, 7 neem, ashwagandha, turmeric, 8 tulsi and giloy, 2 hyper immune serum, [9][10][11] endotoxins such as lipopolysaccharide of E. coli, 9,10,12 low dilutions of H 2 O 2, 13-15 chitosan 16 or silver nanoparticles. 3 Honey with the wonderful broad spectrum antimicrobial action [17][18][19] was tried in mares.…”

Detection and characterization of Lactobacillus spp. in the porcine seminal plasma and their influence on boar semen quality

“…This distinct strain was included in this study because it grew very easily in a liquid culture system and neither affected the viability nor provoked an inflammatory response of the bovine endometrial cells in vitro [ 27 ]. Furthermore, this specific strain also improved the reproductive performance in cows with signs of subclinical endometritis [ 45 ].…”

“…Furthermore, L . buchneri DSM 32407 resulted in shorter days open in cows with signs of subclinical endometritis after intra-uterine application [ 45 ].…”

Isolation and characterization of vaginal Lactobacillus spp. in dromedary camels (Camelus dromedarius): in vitro evaluation of probiotic potential of selected isolates

“…Lactobacillus-based probiotics are contrarily impacted by inflammatory condition in the uterus caused by challenging bacteria resulting in better fertility rates (Peter et al, 2018). Furthermore, LAB isolates can be optimized to get a clinically important product in replacement of antibiotics programs.…”