Influence of intermediate degradation products on the hydrolytic degradation of poly[(<i>rac</i>‐lactide)‐<i>co</i>‐glycolide] at the air–water interface

Schöne, Anne-Christin; Falkenhagen, Sandra; Travkova, Oksana G.; Schulz, Burkhard; Kratz, Karl; Lendlein, Andreas

doi:10.1002/pat.3701

Cited by 7 publications

(9 citation statements)

References 49 publications

(61 reference statements)

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“…The adsorption of PhaF (from the subphase) to the polymer is followed by two in situ methods: Polarization Modulated Infrared Absorption Spectroscopy (PM-IRRAS) and Brewster Angle Microscopy (BAM). The interaction of this protein with a less hydrophobic copolyester, poly[( rac -lactide)- co -glycolide] (PLGA), , will be addressed to substantially improve the understanding on the interplay of polymer hydrophilicity and PhaF adsorption.…”

Section: Introductionmentioning

confidence: 99%

Molecular Insights into the Physical Adsorption of Amphiphilic Protein PhaF onto Copolyester Surfaces

et al. 2019

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Phasins are amphiphilic proteins located at the polymer−cytoplasm interface of bacterial polyhydroxyalkanoates (PHA). The immobilization of phasins on biomaterial surfaces is a promising way to enhance the hydrophilicity and supply celldirecting elements in bioinstructing processes. Optimizing the physical adsorption of phasins requires deep insights into molecular processes during polymer−protein interactions to preserve their structural conformation while optimizing surface coverage. Here, the assembly, organization, and stability of phasin PhaF from Pseudomonas putida at interfaces is disclosed. The Langmuir technique, combined with in situ microscopy and spectroscopic methods, revealed that PhaF forms stable and robust monolayers at different temperatures, with an almost flat orientation of its α-helix at the air−water interface. PhaF adsorption onto preformed monolayers of poly[(3-R-hydroxyoctanoate)-co-(3-R-hydroxyhexanoate)] (PHOHHx), yields stable mixed layers below π = ∼15.7 mN/m. Further insertion induces a molecular reorganization. PHOHHx with strong surface hydrophobicity is a more adequate substrate for PhaF adsorption than the less hydrophobic poly[(rac-lactide)-co-glycolide] (PLGA). The observed orientation of the main axis of the protein in relation to copolyester interfaces ensures the best exposure of the hydrophobic residues, providing a suitable coating strategy for polymer functionalization.

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Section: Introductionmentioning

confidence: 99%

Molecular Insights into the Physical Adsorption of Amphiphilic Protein PhaF onto Copolyester Surfaces

et al. 2019

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“…Nevertheless, a clear trend was observed concerning the glycolic acid content of the copolymer, with higher glycolic acid content resulting in higher rate constants both in enzymatic and in purely hydrolytic degradation . In another effort to further understand the mechanism of PLGA degradation, partly degraded PLGA was studied at the air–water interface . Therefore, PLGA bulk samples were incubated in ultrapure water at 70 °C and samples of both water soluble and insoluble degradation products were taken after different degradation intervals.…”

Section: Key Insights Into Polymer Degradation Based On Langmuir Monomentioning

confidence: 99%

Langmuir Monolayers as Tools to Study Biodegradable Polymer Implant Materials

Machatschek

Schulz

Lendlein

2018

Macromol. Rapid Commun.

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Langmuir monolayers provide a fast and elegant route to analyze the degradation behavior of biodegradable polymer materials. In contrast to bulk materials, diffusive transport of reactants and reaction products in the (partially degraded) material can be neglected at the air–water interface, allowing for the study of molecular degradation kinetics in experiments taking less than a day and in some cases just a few minutes, in contrast to experiments with bulk materials that can take years. Several aspects of the biodegradation behavior of polymer materials, such as the interaction with biomolecules and degradation products, are directly observable. Expanding the technique with surface‐sensitive instrumental techniques enables evaluating the evolution of the morphology, chemical composition, and the mechanical properties of the degrading material in situ. The potential of the Langmuir monolayer degradation technique as a predictive tool for implant degradation when combined with computational methods is outlined, and related open questions and strategies to overcome these challenges are pointed out.

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“…It is assumed that degradation fragments immediately dissolve into the subphase and have not been considered in the degradation process anymore. Recently, the impacts of the intermediate hydrolysis products of PLGA consisting of both water-soluble and water-insoluble components have been investigated [163]. Monolayers based on water-insoluble degradation OLGA fragments reveal highly compressible film behaviour, which significantly differs from the complex behaviour observed for pristine PLGA.…”

Section: Polymer-protein Interactions At the Air-water Interfacementioning

confidence: 99%

Evaluating polymeric biomaterial–environment interfaces by Langmuir monolayer techniques

Schöne

Roch

Schulz

et al. 2017

J. R. Soc. Interface.

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Polymeric biomaterials are of specific relevance in medical and pharmaceutical applications due to their wide range of tailorable properties and functionalities. The knowledge about interactions of biomaterials with their biological environment is of crucial importance for developing highly sophisticated medical devices. To achieve optimal performance, a description at the molecular level is required to gain better understanding about the surface of synthetic materials for tailoring their properties. This is still challenging and requires the comprehensive characterization of morphological structures, polymer chain arrangements and degradation behaviour. The review discusses selected aspects for evaluating polymeric biomaterial-environment interfaces by Langmuir monolayer methods as powerful techniques for studying interfacial properties, such as morphological and degradation processes. The combination of spectroscopic, microscopic and scattering methods with the Langmuir techniques adapted to polymers can substantially improve the understanding of their behaviour.

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Influence of intermediate degradation products on the hydrolytic degradation of poly[(rac‐lactide)‐co‐glycolide] at the air–water interface

Cited by 7 publications

References 49 publications

Molecular Insights into the Physical Adsorption of Amphiphilic Protein PhaF onto Copolyester Surfaces

Molecular Insights into the Physical Adsorption of Amphiphilic Protein PhaF onto Copolyester Surfaces

Langmuir Monolayers as Tools to Study Biodegradable Polymer Implant Materials

Evaluating polymeric biomaterial–environment interfaces by Langmuir monolayer techniques

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