Influence of Insulin on Galactosyl Transferase Activity and Morphology of the Liver Golgi Apparatus in Control and Streptozotocin-Diabetic Rats

Kaczmarski, F; Kordowiak, Anna M; Sarnecka-Keller, M

doi:10.1016/s0344-0338(81)80128-8

Cited by 5 publications

(2 citation statements)

References 20 publications

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“…Statistically significant differences (0.001 < p < 0.01) were demonstrated for D/C, pVC/C, pVD+V/C and D+V/D groups. The first one was in agreement with the results previously obtained [11,12]. It must be stressed that 7 days of oral treatment with 1.8 mmol BMOV solution (group D+V) perfectly normalized the total enzyme activity and improved the morphology of the liver Golgi complex.…”

Section: Fig 1 Compares the Values Of Thesupporting

confidence: 91%

Streptozotocin-induced Alterations in Rat Liver Golgi Complexes are Ameliorated by BMOV [Bis(maltolato)oxovanadium(IV)] Activity

Kordowiak

Dziga²,

Dabroś³

2004

Horm Metab Res

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Twenty years ago, we detected the interdependence between structure and function of rat liver Golgi complexes that are characteristic for streptozotocin diabetes, which served us in further investigations as a useful indicator of the effectiveness of drugs we were testing. This work presented results obtained in eight groups of rats (four control and four diabetic) that were administered orally either bis(maltolato)oxovanadium(IV) [BMOV] or maltol alone. The activities of the rat liver Golgi marker enzyme, galactosyltransferase [GalT], as well as the morphology of Golgi complexes were studied in situ using an electron microscope; parallel estimations of vanadium concentration and phospholipid percentage were made in Golgi-rich preparations isolated from the liver. Our main findings were normalization in diabetic animals orally treated with 1.8 mmol BMOV in 0.09 mol NaCl solutions over seven days, which demonstrated an accompanying increase in phosphatidic acid (PA) percentage (p < 0.05) compared to controls. In the diabetic groups, Pearson's test showed a positive double correlation between GalT activity, vanadium concentration, and PA percentage in Golgi-rich membrane preparations from the liver. Additionally, a negative correlation was found between vanadium concentration and phosphatidylcholine percentage in the fractions.

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Section: Fig 1 Compares the Values Of Thesupporting

confidence: 91%

Streptozotocin-induced Alterations in Rat Liver Golgi Complexes are Ameliorated by BMOV [Bis(maltolato)oxovanadium(IV)] Activity

Kordowiak

Dziga²,

Dabroś³

2004

Horm Metab Res

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“…In our laboratory, such characteristic alterations of the biochemical activity and morphology of liver Golgi complexes in situ were previously found in untreated, streptozotocin (STZ)-induced diabetes in rats [22,23], so they served as a convenient indicator of the effectiveness of tested complexes such as normalising vanadium [13 -15,19, 20] as well as cytoprotective, non-vanadium derivatives [24,25], and used in a study on bis(2,2'-bipyridine)oxovanadium(IV) sulphate [VO(bpy) 2 ], a new synthetic vanadium complex.…”

mentioning

confidence: 95%

The Influence of a New Vanadium Compound, Bis(2,2’-bipyridine)oxovanadium(IV) Sulphate on Liver Golgi Complexes from Control and Streptozotocin-Diabetic Rats

Am¹,

Dabroś²,

B³

2002

Horm Metab Res

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Among the previously studied organic vanadium derivatives showing an anti-diabetic action, we investigated a new complex, bis(2,2'-bipyridine)oxovanadium(IV) sulphate. We tested its ability to normalise parameters previously described for streptozotocin (STZ)-diabetes, such as lower yields of Golgi-rich membrane fraction isolation, decreased activity of Golgi membrane marker enzyme - galactosyltransferase (GalT) - and altered morphology of rat liver Golgi complexes. Oral application as a drinking solution of 1.8 mmol bis(2,2'-bipyridine)oxovanadium(IV) (dissolved in 0.09 M NaCl) caused a similar dispersion of GalT activities in both vanadium treated groups, control and diabetic. Very low activities of the enzyme (characteristic for untreated diabetes) we found only in approximately 35 % of the STZ-diabetic rats treated with the new vanadium compound. The morphology of liver Golgi complexes in diabetic rats treated with bis(2,2'-bipyridine)oxovanadium(IV) sulphate was improved, which manifested itself in the reappearance of vacuoles with VLDL and coated and uncoated secretory vesicles. In view of biochemical and morphological parameters of normalised diabetic rat liver Golgi apparatus, the new vanadium complex was more effective than bis(oxalato)oxovanadium(IV) or bis(kojato)oxovanadium(IV), but in our experimental model, the best anti-diabetic, orally applied drug was the bis(maltolato)oxovanadium(IV) previously investigated.

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Effects of Insulin, Transferrin, Cholera Toxin, and Epidermal Growth Factor on Growth and Morphology of Human Fetal Normal Colon Epithelial Cells

Chopra¹,

Siddiqui²,

Cooney³

1987

Gastroenterology

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Influence of Insulin on Galactosyl Transferase Activity and Morphology of the Liver Golgi Apparatus in Control and Streptozotocin-Diabetic Rats

Cited by 5 publications

References 20 publications

Streptozotocin-induced Alterations in Rat Liver Golgi Complexes are Ameliorated by BMOV [Bis(maltolato)oxovanadium(IV)] Activity

Streptozotocin-induced Alterations in Rat Liver Golgi Complexes are Ameliorated by BMOV [Bis(maltolato)oxovanadium(IV)] Activity

The Influence of a New Vanadium Compound, Bis(2,2’-bipyridine)oxovanadium(IV) Sulphate on Liver Golgi Complexes from Control and Streptozotocin-Diabetic Rats

Effects of Insulin, Transferrin, Cholera Toxin, and Epidermal Growth Factor on Growth and Morphology of Human Fetal Normal Colon Epithelial Cells

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