Influence of insertion protocol and implant shoulder design on inflammatory infiltration and gene expression in peri-implant soft tissue during nonsubmerged dental implant healing

Mueller, Cornelia Katharina; Thorwarth, Michael; Schultze‐Mosgau, Stefan

doi:10.1016/j.tripleo.2010.01.003

Cited by 12 publications

(13 citation statements)

References 27 publications

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“…Hypothesis 1 was confirmed by the data. This periimplant inflammatory infiltration has three different sources: (1) periimplant wound healing [12,15], (2) inflammation elicited by the suturing material [16] and (3) periimplant infection due to plaque accumulation [17].…”

Section: Discussionmentioning

confidence: 99%

“…This lesion might be more pronounced in the flap surgery group due to the larger wound surface, which allows for increased bacteria invasion. Furthermore, the enhanced reepithelization [12], reduced length of the junctional epithelium [7] and increased number of blood vessels [8] in the supracrestal connective tissue following flapless placement might provide a more effective barrier against bacterial invasion then in the flap surgery group.…”

Section: Discussionmentioning

confidence: 99%

“…The sections were deparaffinized in xylene, rehydrated in a graded alcohol series and subjected to standard hematoxylin eosin staining as described previously [12]. Slides were examined quantitatively under a bright field microscope (Axioskop, Zeiss, Oberkochen, Germany) at ×200 original magnification.…”

Section: Standard Histology and Histomorphometrymentioning

confidence: 99%

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Analysis of inflammatory periimplant lesions during a 12-week period of undisturbed plaque accumulation—a comparison between flapless and flap surgery in the mini-pig

2011

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This study's aim was to clarify the influence of soft tissue management on the development of periimplant infection. Four weeks after removal of all maxillary premolars in 12 mini-pigs, four BEGO Semados RI implants were inserted in each maxillary quadrant. Employing a split-mouth design, one quadrant was randomized to flapless insertion while the contralateral side was chosen for flap surgery. Following 1, 2, 4 and 12 weeks of transmucosal implant, healing biopsies were retrieved from the periimplant soft tissue and subjected to further analysis. Histomorphometrically, a significant reduction of transmigration of polymorphonuclear neutrophils (week 1, p = 0.007; week 2, p = 0.021; week 4, p = 0.023; week 12, p = 0.013) as well as the density of the subepithelial inflammatory infiltrates (week 1, p = 0.007; week 2, p = 0.046; week 4, p = 0.003; week 12, p = 0.032) was verified following flapless surgery. Quantification of inducible nitric oxide synthase showed significantly reduced expression in the flapless group 2 (p = 0.027), 4 (p = 0.005) and 12 (p = 0.004) weeks post-insertion. Analysis of CD31 and collagen I immunostained sections revealed more regular capillary distribution as well as higher vessel and collagen density in the flapless group. The data of the present study indicate that flapless placement reduces the incidence of inflammatory periimplant soft tissue lesions during a 12-week period. Considering the beneficial effects of flapless placement on early soft tissue healing and stability, the technique might be preferred in case of an uncomplicated locoregional anatomy with sufficient hard and soft tissue. However, this positive effect might disappear after manipulation of the implant and soft tissue during impression taking or try in of the prosthodontic supraconstruction.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Analysis of inflammatory periimplant lesions during a 12-week period of undisturbed plaque accumulation—a comparison between flapless and flap surgery in the mini-pig

2011

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“…The samples were subjected to standard hematoxylin‐eosin staining as described previously (Mueller et al. ). Ninety histopathological samples were then examined under light microscope at ×80, 200 magnification.…”

Section: Methodsmentioning

confidence: 99%

“…After fixation and paraffin embedding, specimens were cut using microtome, mounted onto glass slides and deparaffinized. The samples were subjected to standard hematoxylin-eosin staining as described previously (Mueller et al 2010). Ninety histopathological samples were then examined under light microscope at 980, 200 magnification.…”

Section: Histopathological Analysismentioning

confidence: 99%