Influence of inflammatory bowel disease on intestinal microflora.

Keighley, M. R. B.; Arabi, Y.; Dimock, F; Burdon, D. W.; Allan, R N; Alexander-Williams, J

doi:10.1136/gut.19.12.1099

Cited by 137 publications

(61 citation statements)

References 18 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…27 Initial studies, determining bacterial colonization in resected ileal and colonic surgical specimens, identified significantly increased E. coli colony counts in CD, but not tissue from patients with UC or normal controls. 28 Recently, higher numbers of E. coli were also identified in neoterminal ileum of CD, especially in early recurrent disease when compared with normal control tissue. 29 Alternatively, PCR-based methods detected E. coli 16S rRNA and DNA more frequently in CD biopsy material than control tissue 30,31 In comparison to tissue infected with other enteric pathogens, immunohistochemistry identified E. coli antigens in biopsy material from 10 out of 16 CD patients, especially in ulcerations, lamina propria, and along fissures.…”

Section: % 50%mentioning

confidence: 99%

Invasive Escherichia coli are a feature of Crohn's disease

Sasaki

Sitaraman

Babbin

et al. 2007

Laboratory Investigation

205

167

View full text Add to dashboard Cite

Section: % 50%mentioning

confidence: 99%

Invasive Escherichia coli are a feature of Crohn's disease

Sasaki

Sitaraman

Babbin

et al. 2007

Laboratory Investigation

205

167

View full text Add to dashboard Cite

“…An increased prevalence of mucosal bacteria has been observed in CD patients, with higher levels of E. coli and Bacteroides species (Keighley et al, 1978;Swidsinski et al, 2002Swidsinski et al, , 2005. Representatives of these bacteria have also been demonstrated to induce colitis when inoculated into germ free animals, however, with conflicting results (Sartor, 2003).…”

Section: Introductionmentioning

confidence: 98%

Molecular analysis of the gut microbiota of identical twins with Crohn's disease

et al. 2008

View full text Add to dashboard Cite

Increasing evidence suggests that a combination of host genetics and the composition of the gut microbiota are important for development of Crohn's disease (CD). Our aim was to study identical twins with CD to determine microbial factors independent of host genetics. Fecal samples were studied from 10 monozygotic twin pairs with CD (discordant n ¼ 6 and concordant n ¼ 4) and 8 healthy twin pairs. DNA was extracted, 16S rRNA genes were PCR amplified and T-RFLP fingerprints generated using general bacterial and Bacteroides group-specific primers. The microbial communities were also profiled based on their percentage G þ C contents. Bacteroides 16S rRNA genes were cloned and sequenced from a subset of the samples. The bacterial diversity in each sample and similarity indices between samples were estimated based on the T-RFLP data using a combination of statistical approaches. Healthy individuals had a significantly higher bacterial diversity compared to individuals with CD. The fecal microbial communities were more similar between healthy twins than between twins with CD, especially when these were discordant for the disease. The microbial community profiles of individuals with ileal CD were significantly different from healthy individuals and those with colonic CD. Also, CD individuals had a lower relative abundance of B. uniformis and higher relative abundances of B. ovatus and B. vulgatus. Our results suggest that genetics and/or environmental exposure during childhood, in part, determine the gut microbial composition. However, CD is associated with dramatic changes in the gut microbiota and this was particularly evident for individuals with ileal CD.

show abstract

“…On the other hand, counts of butyrate-producing Firmicutes (especially those of the Clostridium leptum group) are typically diminished. This may lead to an increased frailty of the intestinal epithelium [91][92][93][94][95][96][97] .…”

Section: The Dysbiosis Theorymentioning

confidence: 99%