Influence of in vitro growth conditions on the photosynthesis and survival of Castanea sativa plantlets during ex vitro transfer

Sáez, Patricia L.; Bravo, León A.; Latsague, Mirtha; Toneatti, Marcelo; Coopman, Rafael E.; Álvarez, Carolina; Sánchez‐Olate, Manuel; Ríos, Darcy

doi:10.1007/s10725-014-9965-1

Cited by 10 publications

(9 citation statements)

References 53 publications

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“…The transpiration rates of all our plants under irradiance close to that used during cultivation were consistent with other studies, which showed significantly higher transpiration in PM plants in comparison to PA ones, e.g. in Ipomoea batatas, tobacco and Castanea sativa (Zobayed et al 2000, Haisel et al 1999, Sáez et al 2015. However, we reject the idea that the stomata of our PM plants were completely dysfunctional.…”

Section: Discussionsupporting

confidence: 92%

“…Moreover, an overall distribution of water is affected by changes in aquaporin‐based hydraulic conductance linked to the tissue sugar levels (once more also via hexokinase signaling) and coordinates the water distribution within the plant body (Kelly et al ). Taking into account that all the studied PA plants had higher instantaneous WUE across the whole interval of 100–800 μmol photons m −2 s −1 , we conclude that PA cultivation lead to improved water balance of the studied plants similar to that shown for C. sativa by Sáez et al ().…”

Section: Discussionsupporting

confidence: 84%

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Mixotrophic in vitro cultivations: the way to go astray in plant physiology

Ševčı́ková

Lhotáková

Hamet

et al. 2019

Physiologia Plantarum

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Rate of photosynthesis and related plant carbohydrate status are crucial factors affecting plant vigor. Sugars providing carbon and energy sources serve also as important signaling molecules governing plant growth and development through a complex regulatory network. These facts are often neglected when mixotrophic cultivation of plants in vitro is used, where artificial exogenous sugar supply hinders studies of metabolism as well as sugar‐driven developmental processes. We compared the growth, selected gas‐exchange parameters and sugar metabolism characteristics in four model plants, potato (Solanum tuberosum ‘Lada’), tobacco (Nicotiana tabacum ‘Samsun’), rapeseed (Brassica napus ‘Asgard’) and strawberry (Fragaria vesca), under both photomixotrophic (PM) and photoautotrophic (PA) conditions. To ensure PA conditions, we used our improved sun caps that serve as gas and light permeable covers for cultivation vessels. We found bigger biomass accumulation, larger leaf areas, higher stomatal conductance and higher instantaneous water use efficiency and lower root sugar contents in PA plants compared to PM ones. However, for other characteristics (root biomass, root/shoot ratio, pigment contents, leaf sugar and starch levels and transpiration rates), a strong species‐dependent reactions to the exogenous sugar supply was noted, which does not allow to create a general view on the overall impact of PM nutrition under in vitro conditions.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 84%

Mixotrophic in vitro cultivations: the way to go astray in plant physiology

Ševčı́ková

Lhotáková

Hamet

et al. 2019

Physiologia Plantarum

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“…8, higher frequencies of rooted and acclimatized shoots could be observed with plant material derived from TIS, although because of the high variability detected between assays, significant differences were only observed in clone 1483. The adaptation of micropropagated chestnut plants to ex vitro conditions is a complex process that may result long and difficult, and it is further complicated by genotypic differences (Gonçalves et al 1998;Ballester et al 1999;Carvalho and Amâncio 2002;Vieitez et al 2007;Sáez et al 2015). Regarding chestnut shoots cultured in TIS, experiments involving a previous phase of hardening are currently in progress to overcome this difficulty.…”

Section: Resultsmentioning

confidence: 99%

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Vidal

Blanco

Cuenca

2015

Plant Cell Tiss Organ Cult

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A protocol for culturing chestnut axillary shoots by temporary immersion in liquid medium was developed. The influence of type of explant, support material, bioreactor, and immersion was investigated for five artificial hybrids and five natural hybrids of Asian and European chestnut selected for resistance to ink disease. The type of explant influenced shoot quality and proliferation rates, and basal explants with callus produced more and longer shoots than apical and nodal segments. Use of rockwool cubes as support material prevented hyperhydricity and allowed proliferation of explants in Murashige and Skoog medium with half-strength nitrates supplemented with 0.22 lM BA and 3 % sucrose, cultured both in plantform TM and RITA Ò vessels with three or six immersions per day and additional aeration of 1 min per hour in the case of plantform TM bioreactors. Basal explants cultured in plantform TM for 5 weeks produced long shoots suitable for rooting, whereas apical and nodal explants cultured in plantform TM or RITA Ò produced shorter shoots that were suitable for maintenance of stock. For most of the clones, similar or higher proliferation rates were observed when cultured in liquid medium than in semisolid medium, with the additional benefit of cost-reduction of the former system. Shoots developed in liquid medium were submitted to ex vitro root induction by dipping in indole-3-butyric acid, and acclimatized under greenhouse conditions. This is the first demonstration of the production of chestnut plantlets from shoots cultured in liquid medium, and the protocol presented here shows good potential for application in large-scale propagation.

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“…In vitro plants are subjected to a stressful environment that has an enormous impact on their metabolite profile [ 138 ], as well as on different photosynthetic parameters [ 139 ], that despite the lack of a proper rooting system might also affect the acclimation success. Indeed, in vitro plants need to develop proper cuticles and epicuticular waxes that help them to stabilize their water apparatus [ 140 ].…”

Section: Root System Architecture and Functionality: In Vitro Vs mentioning

confidence: 99%

Recent Advances in Adventitious Root Formation in Chestnut

Vielba

Vidal

José

et al. 2020

Plants

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The genus Castanea includes several tree species that are relevant because of their geographical extension and their multipurpose character, that includes nut and timber production. However, commercial exploitation of the trees is hindered by several factors, particularly by their limited regeneration ability. Regardless of recent advances, there exists a serious limitation for the propagation of elite genotypes of chestnut due to decline of rooting ability as the tree ages. In the present review, we summarize the research developed in this genus during the last three decades concerning the formation of adventitious roots (ARs). Focusing on cuttings and in vitro microshoots, we gather the information available on several species, particularly C. sativa, C. dentata and the hybrid C.sativa × C. crenata, and analyze the influence of several factors on the achievements of the applied protocols, including genotype, auxin treatment, light regime and rooting media. We also pay attention to the acclimation phase, as well as compile the information available about biochemical and molecular related aspects. Furthermore, we considerate promising biotechnological approaches that might enable the improvement of the current protocols.

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Influence of in vitro growth conditions on the photosynthesis and survival of Castanea sativa plantlets during ex vitro transfer

Cited by 10 publications

References 53 publications

Mixotrophic in vitro cultivations: the way to go astray in plant physiology

Mixotrophic in vitro cultivations: the way to go astray in plant physiology

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Recent Advances in Adventitious Root Formation in Chestnut

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