Influence of Implant Material and Surface on Mode and Strength of Cell/Matrix Attachment of Human Adipose Derived Stromal Cell

Jung, Susanne; Bohner, Lauren; Hanisch, Marcel; Kleinheinz, Johannes; Sielker, Sonja

doi:10.3390/ijms21114110

Cited by 11 publications

(14 citation statements)

References 35 publications

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“…As some cells remained attached to the implants after removing the implants from the oral mucosa models, it can be interpreted that the bond between the cells and implant surfaces were stronger than the bond between the cells within the oral mucosal model. In line with our findings, Jung et al [ 44 ] recently evaluated the adhesion of different cell lines, including gingival fibroblasts and osteoblasts, to different titanium and zirconia implant surfaces. They showed that the surface texture of the implants significantly affected the cell morphology and cytoskeleton.…”

Section: Discussionsupporting

confidence: 90%

Implant Soft-Tissue Attachment Using 3D Oral Mucosal Models—A Pilot Study

et al. 2020

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Purpose: The aim of this study was to investigate soft-tissue attachment to different metal, ceramic, and polymer implant surfaces using an inflamed, three-dimensional (3D), tissue-engineered, human oral mucosal model, as well as multiple-endpoint qualitative and quantitative biological approaches. Methods: Normal human oral fibroblasts, OKF6/TERT-2 keratinocytes and THP-1 monocytes were cultured, and full-thickness, 3D oral mucosal models were engineered inside tissue culture inserts. Sand-blasted and acid-etched (SLA) and machined (M) titanium–zirconium alloy (TiZr; commercially known as Roxolid; Institut Straumann AG, Switzerland), ceramic (ZrO2), and polyether ether ketone (PEEK) rods (Ø 4 mm × 8 mm) were inserted into the center of tissue-engineered oral mucosa following a Ø 4mm punch biopsy. Inflammation was simulated with addition of the lipopolysaccharide (LPS) of Escherichia coli (E. coli) and tumor necrosis factor (TNF)-alpha to the culture medium. Implant soft-tissue attachment was assessed using histology, an implant pull-test with PrestoBlue assay, and scanning electron microscopy (SEM). Results: Inflamed, full-thickness, 3D human oral mucosal models with inserted implants were successfully engineered and histologically characterized. The implant pull-test with PrestoBlue assay showed higher viability of the tissue that remained attached to the TiZr-SLA surface compared to the other test groups. This difference was statistically significant (p < 0.05). SEM analysis showed evidence of epithelial cell attachment on different implant surfaces. Conclusions: The inflamed, 3D, oral mucosal model has the potential to be used as a suitable in vitro test system for visualization and quantification of implant soft-tissue attachment. The results of our study indicate greater soft tissue attachment to TiZr-SLA compared to TiZr-M, ceramic, and PEEK surfaces.

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Section: Discussionsupporting

confidence: 90%

Implant Soft-Tissue Attachment Using 3D Oral Mucosal Models—A Pilot Study

et al. 2020

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“…Vinculin and actin play an important role in cell-cell and cell-matrix adhesion. Both are an important component of focal adhesion, which is critical for tissue remodeling, cell migration and other homeostatic processes [39][40][41][42]. Our immunofluorescence experiments revealed that on MD the HGF-1 cells had a spindle-shaped morphology and were aligned along the grooves.…”

Section: Discussionmentioning

confidence: 74%

Effect of a Nanostructured Titanium Surface on Gingival Cell Adhesion, Viability and Properties against P. gingivalis

et al. 2021

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Objectives: The transgingival part of titanium implants is either machined or polished. Cell-surface interactions as a result of nano-modified surfaces could help gingival fibroblast adhesion and support antibacterial properties by means of the physico-mechanical aspects of the surfaces. The aim of the present study was to determine how a nanocavity titanium surface affects the viability and adhesion of human gingival fibroblasts (HGF-1). Additionally, its properties against Porphyromonas gingivalis were tested. Material and Methods: Two different specimens were evaluated: commercially available machined titanium discs (MD) and nanostructured discs (ND). To obtain ND, machined titanium discs with a diameter of 15 mm were etched with a 1:1 mixture of 98% H2SO4 and 30% H2O2 (piranha etching) for 5 h at room temperature. Surface topography characterization was performed via scanning electron microscopy (SEM) and atomic force microscopy (AFM). Samples were exposed to HGF-1 to assess the effect on cell viability and adhesion, which were compared between the two groups by means of MTT assay, immunofluorescence and flow cytometry. After incubation with P. gingivalis, antibacterial properties of MD and ND were determined by conventional culturing, live/dead staining and SEM. Results: The present study successfully created a nanostructured surface on commercially available machined titanium discs. The etching process created cavities with a 10–20 nm edge-to-edge diameter. MD and ND show similar adhesion forces equal to about 10–30 nN. The achieved nanostructuration reduced the cell alignment along machining structures and did not negatively affect the proliferation of gingival fibroblasts when compared to MD. No differences in the expression levels of both actin and vinculin proteins, after incubation on MD or ND, were observed. However, the novel ND surface failed to show antibacterial effects against P. gingivalis. Conclusion: Antibacterial effects against P. gingivalis cannot be achieved with nanocavities within a range of 10–20 nm and based on the piranha etching procedure. The proliferation of HGF-1 and the expression levels and localization of the structural proteins actin and vinculin were not influenced by the surface nanostructuration. Further studies on the strength of the gingival cell adhesion should be performed in the future. Clinical relevance: Since osseointegration is well investigated, mucointegration is an important part of future research and developments. Little is known about how nanostructures on the machined transgingival part of an implant could possibly influence the surrounding tissue. Targeting titanium surfaces with improved antimicrobial properties requires extensive preclinical basic research to gain clinical relevance.

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“…Cells adhere to the ECM via an integrin-mediated adhesion that connects ECM to the cytoskeleton, and integrins serve as receptors for ECM proteins [ 45 ]. In order to ameliorate the regeneration, it is essential to evaluate the cell binding strength to the implant material surface [ 46 ]. In particular, some expressed proteins are vital for the osteogenesis process, RUNX2 is essential and required for physiologic bone formation, osteopontin is a protein involved in normal calcifications and SPARC is one of the most abundant non-collagenous proteins expressed in the mineralized tissues [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences

et al. 2021

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The surface structure of the titanium dental implants can modulate the activity of mesenchymal stem cells in order to promote the upregulation of osteoblastic related genes and the release of extracellular matrix (ECM) components. The present work was focused on the in vitro evaluation of the interaction of human periodontal ligament stem cells (hPDLSCs) and two different implant titanium surfaces topography (CTRL and TEST). This study was aimed at analyzing the cytotoxicity of the dental implant surfaces, the cellular adhesion capacity, and the improvement in the release of ECM molecules in an in vitro model. These parameters were carried out by means of the microscopic evaluation, viability assays, immunofluorescence, Western blot and RT-PCR investigations. The knowledge of the cell/implant interaction is essential for implant healing in order to obtain a more performing surfaces that promote the ECM release and provide the starting point to initiate the osseointegration process.

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Influence of Implant Material and Surface on Mode and Strength of Cell/Matrix Attachment of Human Adipose Derived Stromal Cell

Cited by 11 publications

References 35 publications

Implant Soft-Tissue Attachment Using 3D Oral Mucosal Models—A Pilot Study

Implant Soft-Tissue Attachment Using 3D Oral Mucosal Models—A Pilot Study

Effect of a Nanostructured Titanium Surface on Gingival Cell Adhesion, Viability and Properties against P. gingivalis

Enhanced Extracellular Matrix Deposition on Titanium Implant Surfaces: Cellular and Molecular Evidences

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