Influence of Immunopotentiators on the Antiporin Immunoglobulin G Subclass: Distribution and Protective Immunity Against Murine Salmonellosis

Nandakumar, Kutty Selva; Muthukkaruppan, Veerappan

doi:10.1046/j.1365-3083.1999.00576.x

Cited by 4 publications

(3 citation statements)

References 31 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have demonstrated that salmonella porins induce the production of predominantly IgG1 after 30 days in BALB/c mice 42 . We have recently reported that human volunteers vaccinated with S .…”

Section: Introductionmentioning

confidence: 85%

“…This notable immunogenicity of porins to sustain bactericidal antibody memory response could be explained by either of the following non‐exclusive mechanisms. First, because salmonella porins are good immunogens 20,21 , 27,33 , 36–42 and porins from other pathogens have been identified as TLR2 agonists 29–31 . Thus, during the initial immune response, it is possible that salmonella porins might be simultaneously recognized by B‐cell receptors and innate receptors, such as TLRs, thereby inducing the differentiation of naive B cells into effector antibody‐secreting plasma cells.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Salmonella porins induce a sustained, lifelong specific bactericidal antibody memory response

et al. 2005

View full text Add to dashboard Cite

SummaryWe examined the ability of porins from Salmonella enterica serovar typhi to induce a long-term antibody response in BALB/c mice. These porins triggered a strong lifelong production of immunoglobulin G (IgG) antibody in the absence of exogenous adjuvant. Analysis of the IgG subclasses produced during this antibody response revealed the presence of the subclasses IgG2b, IgG1, IgG2a and weak IgG3. Despite the high homology of porins, the long-lasting anti-S. typhi porin sera did not cross-react with S. typhimurium. Notably, the antiporin sera showed a sustained lifelong bactericidal-binding activity to the wild-type S. typhi strain, whereas porin-specific antibody titres measured by enzyme-linked immunosorbent assay (ELISA) decreased with time. Because our porin preparations contained the outer membrane proteins C and F (OmpC and OmpF), we evaluated the individual contribution of each porin to the long-lasting antibody response. OmpC and OmpF induced long-lasting antibody titres, measured by ELISA, which were sustained for 300 days. In contrast, although OmpC induced sustained high bactericidal antibody titres for 300 days, postimmunization, the bactericidal antibody titre induced by OmpF was not detected at day 180. These results indicate that OmpC is the main protein responsible for the antibody-mediated memory bactericidal response induced by porins. Taken together, our results show that porins are strong immunogens that confer lifelong specific bactericidal antibody responses in the absence of added adjuvant.

show abstract

Section: Introductionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Salmonella porins induce a sustained, lifelong specific bactericidal antibody memory response

et al. 2005

View full text Add to dashboard Cite

show abstract

“…Water-soluble, non-toxic and synthetic polyelectrolytes were found to be efficient immunomodulators [88]. A more prominent increase in antibody responses was achieved by complexing an outer membrane protein of Salmonella, porin, with the heteropolymer, polyoxydonium [89]. Similarly, Salmonella O and H antigens complexed with polyelectrolytes have been shown to induce several-fold higher antibody responses against respective antigens [90].…”

Section: Polyelectrolytesmentioning

confidence: 99%

Applications of polymeric adjuvants in studying autoimmune responses and vaccination against infectious diseases

Shakya

Nandakumar

2013

J. R. Soc. Interface.

View full text Add to dashboard Cite

Polymers as an adjuvant are capable of enhancing the vaccine potential against various infectious diseases and also are being used to study the actual autoimmune responses using self-antigen(s) without involving any major immune deviation. Several natural polysaccharides and their derivatives originating from microbes and plants have been tested for their adjuvant potential. Similarly, numerous synthetic polymers including polyelectrolytes, polyesters, polyanhydrides, non-ionic block copolymers and external stimuli responsive polymers have demonstrated adjuvant capacity using different antigens. Adjuvant potential of these polymers mainly depends on their solubility, molecular weight, degree of branching and the conformation of polymeric backbone. These polymers have the ability not only to activate humoral but also cellular immune responses in the host. The depot effect, which involves slow release of antigen over a long duration of time, using different forms (particulate, solution and gel) of polymers, and enhances the co-stimulatory signals for optimal immune activation, is the underlying principle of their adjuvant properties. Possibly, polymers may also interact and activate various toll-like receptors and inflammasomes, thus involving several innate immune system players in the ensuing immune response. Biocompatibility, biodegradability, easy production and purification, and non-toxic properties of most of the polymers make them attractive candidates for substituting conventional adjuvants that have undesirable effects in the host.

show abstract

Vaccination of Chickens with Liposomal Inactivated Avian Pathogenic Escherichia coli (APEC) Vaccine by Eye Drop or Coarse Spray Administration

et al. 2009

View full text Add to dashboard Cite

One hundred and twenty 30-day-old specific-pathogen-free chickens were inoculated with the liposomal inactivated avian pathogenic Escherichia coli (APEC) vaccine by eye drop or coarse spraying. All of the chickens produced anti-lipopolysaccharide antibodies of the IgG subclass in their sera as well as IgA antibodies in their oral mucus. The results demonstrated a rise in antibodies in the serum of chickens administered the APEC vaccine through nonparenteral mucosal routes. Bacterial counts in the blood decreased, and clinical signs were moderated in the vaccinated chickens after challenge with a strain of APEC. No harmful effects from the vaccination were observed. The liposomal inactivated APEC vaccine described in this paper would contribute to a practical method of control for avian colibacillosis.

show abstract

Influence of Immunopotentiators on the Antiporin Immunoglobulin G Subclass: Distribution and Protective Immunity Against Murine Salmonellosis

Cited by 4 publications

References 31 publications

Salmonella porins induce a sustained, lifelong specific bactericidal antibody memory response

Salmonella porins induce a sustained, lifelong specific bactericidal antibody memory response

Applications of polymeric adjuvants in studying autoimmune responses and vaccination against infectious diseases

Vaccination of Chickens with Liposomal Inactivated Avian Pathogenic Escherichia coli (APEC) Vaccine by Eye Drop or Coarse Spray Administration

Contact Info

Product

Resources

About