Influence of icing on muscle regeneration after crush injury to skeletal muscles in rats

Takagi, Ryo; Fujita, Naoto; Arakawa, Takamitsu; Kawada, Shigeo; Ishii, Naokata; Miki, Akinori

doi:10.1152/japplphysiol.01187.2010

Cited by 94 publications

(111 citation statements)

References 33 publications

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“…The parameters that had the greatest influence on predicted M1 macrophage agent populations are listed in table 2 . Since numerous studies have explored M1 macrophage population levels following injury [St Pierre and Tidball, 1994;Tidball et al, 1999;Takagi et al, 2011;Mounier et al, 2013], we sought to identify which ABM parameters would yield model outputs that best matched M1 population dynamics. To accomplish this, we used a GA to identify the combination of parameter values ( table 2 ) that best fit experimental measurements of M1 macrophage populations, as described below.…”

Section: Model Parameterizationmentioning

confidence: 99%

Computational Modeling of Muscle Regeneration and Adaptation to Advance Muscle Tissue Regeneration Strategies

Martin¹,

Virgilio²,

Peirce³

et al. 2016

Cells Tissues Organs

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Skeletal muscle has an exceptional ability to regenerate and adapt following injury. Tissue engineering approaches (e.g. cell therapy, scaffolds, and pharmaceutics) aimed at enhancing or promoting muscle regeneration from severe injuries are a promising and active field of research. Computational models are beginning to advance the field by providing insight into regeneration mechanisms and therapies. In this paper, we summarize the contributions computational models have made to understanding muscle remodeling and the functional implications thereof. Next, we describe a new agent-based computational model of skeletal muscle inflammation and regeneration following acute muscle injury. Our computational model simulates the recruitment and cellular behaviors of key inflammatory cells (e.g. neutrophils and M1 and M2 macrophages) and their interactions with native muscle cells (muscle fibers, satellite stem cells, and fibroblasts) that result in the clearance of necrotic tissue and muscle fiber regeneration. We demonstrate the ability of the model to track key regeneration metrics during both unencumbered regeneration and in the case of impaired macrophage function. We also use the model to simulate regeneration enhancement when muscle is primed with inflammatory cells prior to injury, which is a putative therapeutic intervention that has not yet been investigated experimentally. Computational modeling of muscle regeneration, pursued in combination with experimental analyses, provides a quantitative framework for evaluating and predicting muscle regeneration and enables the rational design of therapeutic strategies for muscle recovery.

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Section: Model Parameterizationmentioning

confidence: 99%

Computational Modeling of Muscle Regeneration and Adaptation to Advance Muscle Tissue Regeneration Strategies

Martin¹,

Virgilio²,

Peirce³

et al. 2016

Cells Tissues Organs

View full text Add to dashboard Cite

show abstract

“…We also investigated the effect of MENS combined with icing, a common first-aid treatment in sports-associated injuries, on the regeneration of injured skeletal muscles 24) . While icing on injured tissues immediately after an injury is generally believed to suppress pain and swelling, an icing-associated delay of skeletal muscle repair has been reported 25,26) . Our experiment on the combined treatment of MENS and icing had no observable effect on the regenerative process of injured skeletal muscle 24) .…”

Section: D) Skeletal Muscle Injuriesmentioning

confidence: 99%

New aspects of microcurrent electrical neuromuscular stimulation in sports medicine

Fujiya

Goto

2016

JPFSM

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Sports-associated injuries often involve trauma to soft tissues such as ligaments, tendons, skeletal muscle, and skin. A shortened recovery process for injured tissues is of great interest to athletes, as injury-associated inactivity depresses both sports performance and physical fitness. Recently proposed treatments to accelerate tissue repair include microcurrent electrical neuromuscular stimulation (MENS), low-intensity pulsed ultrasound (LIPUS), hyperbaric oxygen (HBO), and autologous platelet-rich plasma (PRP). Among these treatments, MENS has been applied to alleviate pain and reduce swelling following sports-associated injuries of tendons and ligaments. MENS is reported to stimulate the regeneration of skeletal muscles, a part of the body commonly injured in sports. MENS is expected to soon become a standard therapy for accelerating the repair of injured skeletal muscles and other soft tissues. In this review, we provide an overview of MENS and briefly describe several other proposed treatments for sports-associated injuries.

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“…This is the first study to investigate the combined effects of MENS and icing on the regeneration of injured skeletal muscle. Recent evidences show that icing suppressed the regeneration of injured skeletal muscle in rats 17) and mice 18) . Although the effects of icing alone on the regeneration of injured TA muscle was not investigated in the present study, icing may depress the regeneration of injured TA muscle.…”

Section: Combined Effects Of Mens With Icingmentioning

confidence: 99%

Regeneration of Injured Tibialis Anterior Muscle in Mice in Response to Microcurrent Electrical Neuromuscular Stimulation with or without Icing

Yoshida

Fujiya

Goto

et al. 2015

J St. Marianna Univ

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Objective: Cold therapy, so-called icing, is often used immediately after muscle injuries as first aid for the suppression of inflammation and pain relief. Recent evidences show icing retards the regeneration of injured skeletal muscle. On the other hand, microcurrent electrical neuromuscular stimulation (MENS) promotes the regeneration of injured skeletal muscle. In this study, we investigated the effects of the MENS with or without icing on the regeneration of injured skeletal muscle. Methods: Eight-week-old male mice (C57BL/6J) were divided into 4 groups: control (C), cardiotoxin (CTX) injected (X), CTX injected with MENS (XM), and CTX injected with combined treatments with icing and MENS (XIM) groups. Necrosis-regeneration cycle was induced by an intramuscular injection of CTX into tibialis anterior (TA) muscles except for C group. After CTX-injection, the hindlimbs of the mice were soaked in ice-cold water (4ºC) for 20 minutes under anesthesia (XIM). After the treatments, both right and left hindlimbs of the mice in XIM and XM groups were treated with MENS (10 µA, 0.3 Hz, 250 msec) for 60 min a day and 3 days per week for 1 or 3 weeks. One and three weeks after CTX injection, TA muscles were dissected. Results: MENS with or without icing facilitated the recovery of muscle protein content and muscle fiber morphology including mean fiber cross-sectional areas of injured TA muscle, compared with non-treated condition. These facilitating effects of MENS with or without icing were accompanied with the increase in the relative number of Pax7-positive nuclei, namely satellite cells. Judging from fiber morphology, MENS with icing had enhanced stimulating effects on the regeneration of injured skeletal muscle, compared with MENS-treated condition. Conclusion: Evidence suggested that MENS with or without icing facilitated the regeneration of injured TA muscle. A combination treatment of MENS with icing might be a useful therapy for sports-related skeletal muscle injuries.

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Influence of icing on muscle regeneration after crush injury to skeletal muscles in rats

Cited by 94 publications

References 33 publications

Computational Modeling of Muscle Regeneration and Adaptation to Advance Muscle Tissue Regeneration Strategies

Computational Modeling of Muscle Regeneration and Adaptation to Advance Muscle Tissue Regeneration Strategies

New aspects of microcurrent electrical neuromuscular stimulation in sports medicine

Regeneration of Injured Tibialis Anterior Muscle in Mice in Response to Microcurrent Electrical Neuromuscular Stimulation with or without Icing

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