Influence of Hydrogen Peroxide, Lactic Acid, and Surfactants from Vaginal Lactobacilli on the Antibiotic Sensitivity of Opportunistic Bacteria

Abstract

We studied as hydrogen peroxide, lactic acid, or surfactants from clinical isolates of vaginal lactobacilli and cell-free supernatants from probiotic strain LCR35 can influence on the sensitivity of opportunistic bacteria to antibiotics. We found that the most effective in increasing sensitivity to antibiotics were hydrogen peroxide and surfactants or their combination but no lactic acid. In some cases, the effect of the composition of hydrogen peroxide and surfactants was clearly higher than the sum of effects of these substances alone. With using of the supernatant of LCR35 was shown that the combination of surfactant and lactate has greater effect compared with surfactants alone. In concluding, metabolites of vaginal lactobacilli are suitable for the role of "antibiotic assistants" and it can help solve the problems the antibiotic resistance.

Probiotics and competitive exclusion of pathogens in shrimp aquaculture

“…Kongnum and Hongpattarakere (2012) found that hydrogen peroxide was a major agent contributing to the antibacterial effect of Lactobacillus plantarum MRO3.12. Interestingly, Sgibnev and Kremleva (2017) found that hydrogen peroxide produced by probiotic vaginal lactobacilli were effective in increasing sensitivity to antibiotics, suggesting that hydrogen peroxide producing LAB in combination with antibiotic‐producing species may be more effective for pathogenic growth inhibition. The antimicrobial activity of LAB shrimp probiotic Streptococcus phocae PI80 has been shown to be due to the production of the bacteriocin Phocaecin PI80, which inhibits the growth of a range of Gram‐positive and Gram‐negative bacteria, including Listeria monocytogenes , Vibrio spp., Aeromonas hydrophila and Pseudomonas aeroginosa (Satish Kumar & Arul, 2009).…”

Gardnerella vaginalis and Neisseria gonorrhoeae Are Effectively Inhibited by Lactobacilli with Probiotic Properties Isolated from Brazilian Cupuaçu (Theobroma grandiflorum) Fruit

“…The literature suggests that the main advantages associated with H 2 O 2 activity are its ability to cross the cell membrane of the pathogen, to damage the membrane, proteins, DNA, and enzymes by the formation of free radicals and the anti-inflammatory stimulus of the vaginal mucosa [ 79 – 81 ]. Furthermore, the joint action of metabolites, such as H 2 O 2 , organic acids, bacteriocins, and biosurfactants, has already been attributed as a potent antibiotic-assisting tool to contain genital infections and minimize resistance to antibiotics [ 82 ].…”

Activity of metabolites produced by new strains of Lactobacillus in modified de Man, Rogosa and Sharpe (MRS) medium against multidrug-resistant bacteria

“…Several studies have been published on antimicrobial activity of lactic acid produced by lactobacilli against pathogen microorganisms (Şanlıbaba and Güçer, 2015;Arqués et al, 2015;Sgibnev and Kremleva, 2016). The antimicrobial activity of organic acids is directly related to the pH reduction and its ability to dissociate.…”

“…The beneficial action of lactobacilli has been widely described in several reports which focus on their viable cells and cell free supernatants (CFS) (Mariam et al, 2014;Lau and Liong, 2014). The CFS is a well-known source of bioactive compounds such as antioxidants, bacteriocins, surfactants, organic acids, H 2 O 2 , CO 2, and low molecular weight peptides (Lau and Liong, 2014;Sgibnev and Kremleva, 2016). Their biosynthesis depends on the factors linked to growth such as pH, temperature, O 2 tension, and culture medium composition (Zalán et al, 2010).…”