Influence of hydrogen peroxide in drinking water on diazepam pharmacokinetics in chicks

Abstract

Aim: Stressful conditions affect drug pharmacokinetics and pharmacodynamics. This study examines the effect of hydrogen peroxide (H O ) in drinking water on the pharmacokinetics of diazepam in a chick model of oxidative stress. 0.5% v/v drinking solution for two weeks in order to produce oxidative stress. On treatment days 7-14, the chicks were treated with a sedative dose of diazepam at 10 mg/kg, intramuscularly. Blood samples were obtained from chicks (5/each sampling time) at times of between 0.17 to 4 h. The concentrations of diazepam in the plasma were determined by an HPLC method with UV-detector. Pharmacokinetic parameters of diazepam were calculated from the mean drug concentrations in the plasma by a non-compartmental analysis using a Windows-based computer program. decreasing its elimination from the body.

Neuroacting drugs and its pharmacological response in relation to different stress status: A review

“…H 2 O 2 reduces the effectiveness of dehydrogenases in the Krebs cycle, energy production, stimulates the growth factor and the receptor of the Aspartate neurotransmitter, which leads to an elevation in the calcium influx into the nerve cell and has a vital role for the serotonin receptor of rat brain, leading to poor behavior (Patockova et al, 2003;Sayre et al, 2008). Experimentally, H 2 O 2 causes OS in chickens when given at 0.5% in water along with fourteen days and causes a neurobehavioral change in the open field activity (Mousa, 2012;2014, Mousa andMohammad, 2012a;b, Mousa, 2021;Mousa et al, 2021), besides modifying the pharmacokinetics (Mousa and Mohammad, 2012c).…”

“…Stress modifies drug disposition and availability to the target receptors by increasing the absorption of the drug from the site of treatment and alters their distribution by the destruction of the protein binding while decreasing the metabolism by affecting the cytochrome P 450 enzymes responsible for drug elimination and effect termination and later decrease drug excretion (Mohammad et al, 1999;Mousa, 2012;Mousa and Mohammad, 2012c).…”