Influence of hydration on segmental chain librations and dynamical transition in lipid bilayers

Aloi, Erika; Bartucci, Rosa

doi:10.1016/j.bbamem.2021.183805

Cited by 4 publications

(1 citation statement)

References 47 publications

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“…On the whole, the differences in the librational dynamics in the various bilayers are attributable mostly to the variations in the angular amplitude rather than in the rotational correlation time. It is interesting to point out that the temperature dependence of α 2 shows close similarities with that of the mean-square atomic displacement r 2 measured in neutron studies (Fenimore et al, 2004;Dzuba, 2007;Golysheva et al, 2017;Peters et al, 2017;Golysheva et al, 2018;Aloi and Bartucci, 2022). Both curves show a rapid increase at a temperature in the range of 200 K ascribed to the dynamical transition from harmonic to anharmonic diffusive motion.…”

Section: Segmental Chain Librations Of Lipids In Model Membranessupporting

confidence: 75%

Librational Dynamics of Spin-Labeled Membranes at Cryogenic Temperatures From Echo-Detected ED-EPR Spectra

Bartucci

Aloi²

2022

Front. Mol. Biosci.

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Methods of electron spin echo of pulse electron paramagnetic resonance (EPR) spectroscopy are increasingly employed to investigate biophysical properties of nitroxide-labeled biosystems at cryogenic temperatures. Two-pulse echo-detected ED-spectra have proven to be valuable tools to describe the librational dynamics in the low-temperature phases of both lipids and proteins in membranes. The motional parameter, α2τC, given by the product of the mean-square angular amplitude, α2, and the rotational correlation time, τC, of the motion, is readily determined from the nitroxide ED-spectra as well as from the W-relaxation rate curves. An independent evaluation of α2 is obtained from the motionally averaged 14N-hyperfine splitting separation in the continuous wave cw-EPR spectra. Finally, the rotational correlation time τC can be estimated by combining ED- and cw-EPR data. In this mini-review, results on the librational dynamics in model and natural membranes are illustrated.

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Section: Segmental Chain Librations Of Lipids In Model Membranessupporting

confidence: 75%

Librational Dynamics of Spin-Labeled Membranes at Cryogenic Temperatures From Echo-Detected ED-EPR Spectra

Bartucci

Aloi²

2022

Front. Mol. Biosci.

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In-plane and out-of-plane gigahertz sound velocities of saturated and unsaturated phospholipid bilayers from cryogenic to room temperatures

Dobrynina

Zykova

Surovtsev

2023

Chemistry and Physics of Lipids

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Low-temperature librations and dynamical transition in proteins at differing hydration levels

Aloi

Bartucci

Guzzi

2022

Biomolecular Concepts

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Hydration of water affects the dynamics and in turn the activity of biomacromolecules. We investigated the dependence of the librational oscillations and the dynamical transition on the hydrating conditions of two globular proteins with different structure and size, namely β-lactoglobulin (βLG) and human serum albumin (HSA), by spin-label electron paramagnetic resonance (EPR) in the temperature range of 120–270 K. The proteins were spin-labeled with 5-maleimide spin-label on free cysteins and prepared in the lyophilized state, at low (h = 0.12) and full (h = 2) hydration levels in buffer. The angular amplitudes of librations are small and almost temperature independent for both lyophilized proteins. Therefore, in these samples, the librational dynamics is restricted and the dynamical transition is absent. In the small and compact beta-structured βLG, the angular librational amplitudes increase with temperature and hydrating conditions, whereas hydration-independent librational oscillations whose amplitudes rise with temperature are recorded in the large and flexible alpha-structured HSA. Both βLG and HSA at low and fully hydration levels undergo the dynamical transition at about 230 K. The overall results indicate that protein librational dynamics is activated at the low hydration level h = 0.12 and highlight biophysical properties that are common to other biosamples at cryogenic temperatures.

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Influence of hydration on segmental chain librations and dynamical transition in lipid bilayers

Cited by 4 publications

References 47 publications

Librational Dynamics of Spin-Labeled Membranes at Cryogenic Temperatures From Echo-Detected ED-EPR Spectra

Librational Dynamics of Spin-Labeled Membranes at Cryogenic Temperatures From Echo-Detected ED-EPR Spectra

In-plane and out-of-plane gigahertz sound velocities of saturated and unsaturated phospholipid bilayers from cryogenic to room temperatures

Low-temperature librations and dynamical transition in proteins at differing hydration levels

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