Influence of hyaluronic acid on bacterial and fungal species, including clinically relevant opportunistic pathogens

Ardizzoni, Andrea; Neglia, Rachele Giovanna; Baschieri, Maria Cristina; Cermelli, Claudio; Caratozzolo, M.; Righi, Elena; Palmieri, Beniamino; Blasi, Elisabetta

doi:10.1007/s10856-011-4408-2

Cited by 99 publications

(72 citation statements)

References 16 publications

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“…It is a main component of extracellular tissues abundant in skin, connective tissue, synovial fluid, and vitreous humor. 13 HA polymers organize into a reticular structure and produce a molecular framework. 20 This characteristic enables HA to be used as an ingredient in several artificial tears.…”

Section: Yadav Chuck and Parkmentioning

confidence: 99%

“…22 Dose-dependent antibacterial effects of HA were reported in some gram-positive (Staphylococcus epidermidis) and gram-negative (Escherichia coli) bacteria, and the addition of HA in the culture medium can invoke relatively inhibitory effects on growth of planktonic P. aeruginosa, although the exact mechanism remains unclear. 13,23 Further research regarding the antimicrobial effects of HA is necessary, but is beyond the scope of this article.…”

Section: Yadav Chuck and Parkmentioning

confidence: 99%

“…However, some compositions such as hyaluronic acid (HA) or carboxymethylcellulose (CMC) can be used as an energy source by some bacteria. 13,14 To our knowledge, there is no published report assessing biofilm formation in artificial tears of various compositions. In this study, we evaluated the effect of various commercial artificial teardrops on P. aeruginosa biofilm formation on silicone hydrogel lenses.…”

Section: Introductionmentioning

confidence: 99%

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Composition of Artificial Tear Solution AffectsIn Vitro Pseudomonas aeruginosaBiofilm Formation on Silicone Hydrogel Lens

Yadav

Chuck

Park

2013

Journal of Ocular Pharmacology and Therapeutics

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Section: Yadav Chuck and Parkmentioning

confidence: 99%

Section: Yadav Chuck and Parkmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Composition of Artificial Tear Solution AffectsIn Vitro Pseudomonas aeruginosaBiofilm Formation on Silicone Hydrogel Lens

Yadav

Chuck

Park

2013

Journal of Ocular Pharmacology and Therapeutics

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“…Ardizzoni et al 41 reported such effects for HA. In particular staphylococci, enterococci, Streptococcus mutans, two Escherichia coli strains, Pseudomonas aeruginosa, Candida glabrata and C. parapsilosis displayed for HA dose dependent growth inhibition.…”

Section: Discussionmentioning

confidence: 93%

Biological properties of a novel coladerm-beta glucan membrane.In vitro assessment using human fibroblasts

Jantová¹,

Bakoš²,

Bírošová³

et al. 2015

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Aim. The purpose of this study was to prepare a coladerm-beta glucan membrane (CBGM) and to evaluate its biocompatibility, cytotoxicity, antimicrobial activity, genotoxicity and mutagenicity. Methods. The biocompatibility of the membrane was studied on the base of cell adhesion and colonization of human fibroblasts on the biomaterial surface by light microscopy. The MTT test and LDH level determination in the culture medium removed from the control and cells treated on the membrane, were used for viability and cytotoxic evaluations. Flow cytometry and gel electrophoresis were used for analysis of cell cycle and death. The antimicrobial activity of CBGM was tested using the qualitative dilution method. Ames bacteria gene mutation test and Comet assay were used for mutagenic and genotoxic studies. Results. MTT and LDH tests confirmed that CBGM is a non-toxic biomaterial. Flow cytometry and gel electrophoresis demonstrated that the membrane did not affect the cell cycle and did not induce either necrotic or apoptotic cell death. CBGM exhibited antibacterial activity against G -bacteria E. sakazakii, S. marcescens, E. coli and agains G + sporogenic bacteria B. cereus. No antifungal activity was detected. The membrane did not induce mutagenicity in the bacterial reverse mutation test in Salmonella Typhimurium strains. Similarly, the comet assay showed that the tested fibroblast cells growing with/without the membrane did not show any statistically significant DNA damage. Conclusions. The CBGM has good biocompatibility, no cytotoxicity/genotoxicity/mutagenicity and it can be included as a potential scaffold for tissue engineering.

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“…15 In terms of bacterial and fungal species, staphylococci, enterococci, Streptococcus mutans, two Escherichia coli strains, Pseudomonas aeruginosa, Candida glabrata, and Candida parapsilosis exhibit hyaluronic acid dose-dependent growth inhibition, while E. coli ATCC 13768 and Candida albicans do not exhibit any effect, and Streptococcus sanguinis is favored by high hyaluronic acid dose. 16 In summary, hyaluronic acid produces changes in the immune system, inflammatory mediators, and markers of oxidative stress, and may interact with pathogens. This evidence should be taken into account when performing hyaluronic acid-based delivery of drugs and medical procedures in clinical practice.…”

Section: Hyaluronic Acid and Nanomedicinementioning

confidence: 99%

Probable biofilm formation in the cheek as a complication of soft tissue filler resulting from improper endodontic treatment of tooth 16

Marusza

Młynarczyk²,

Olszański³

et al. 2012

IJN

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Injectable filling agents offer the promise of a better appearance without surgery and, among them, hyaluronic acid is the most commonly used. Although complications are rare, it is necessary to know the possible side effects and complications in order to be prepared for their management. That is why many researchers have been focusing on the interactions between hyaluronic acid and pathogens, inflammatory mediators, the immune system, and markers of oxidative stress to achieve efficient drug delivery, given that hyaluronic acid has widening applications in the field of nanomedicine. Here we report the case of a 37-year-old female patient who returned to our clinic with an abscess in her left cheek 3 months after a deep injection of 1 mL of stabilized hyaluronic acid in both cheeks. Steroid and antibiotic therapy was initiated without success, and abscess drainage was performed. Extraction of tooth 16 was performed 11 days after insertion of drains into the abscess. Laboratory blood tests showed acute inflammation of presumed bacterial etiology. Microbiological examination of pus was negative. Bacterial cultures were found in the extracted tooth. After antibiotic therapy, a complete reversal of the pathological process was observed. The present report highlights the need to assess periodontal problems prior to any aesthetic facial treatment. Analyses of further case reports and clinical studies are necessary to understand the potential role of hyaluronic acid in the formation of biofilm, and how to avoid this complication, thereby increasing the safety of hyaluronic acid-based procedures.

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Influence of hyaluronic acid on bacterial and fungal species, including clinically relevant opportunistic pathogens

Cited by 99 publications

References 16 publications

Composition of Artificial Tear Solution AffectsIn Vitro Pseudomonas aeruginosaBiofilm Formation on Silicone Hydrogel Lens

Composition of Artificial Tear Solution AffectsIn Vitro Pseudomonas aeruginosaBiofilm Formation on Silicone Hydrogel Lens

Biological properties of a novel coladerm-beta glucan membrane.In vitro assessment using human fibroblasts

Probable biofilm formation in the cheek as a complication of soft tissue filler resulting from improper endodontic treatment of tooth 16

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