Influence of human rheumatoid factor on numbers of antibody‐producing cells

Brown, Jerry R.; Epstein, Wallace V.

doi:10.1002/art.1780120102

Cited by 6 publications

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“…Patients with gout gave increased rosette counts. These authors suggested that the lymphocyte response of the rheumatoid individual precedes the appearance of humoral rheumatoid factors and that the process may correspond to that found in animals after immunization (Brown and Epstein, 1969).In order to examine the properties and occurrence of rosette-forming lymphocytes, we have tested lymphocyte preparations from normal subjects of various ages, patients suffering from rheumatoid arthritis, and patients with orthopaedic conditions not related to rheumatoid arthritis. …”

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Rosette formation by circulating lymphocytes from rheumatoid and nonrheumatoid subjects.

Lea¹,

Ward²

1972

Annals of the Rheumatic Diseases

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In the majority of patients with rheumatoid arthritis the immune response is most readily detected as a production of rheumatoid factors, IgM antibodies, which agglutinate to high titre erythrocytes coated with IgG antibodies from man, rabbit, or sheep, etc. Generally, only low titres of rheumatoid factors are found in nonrheumatoid individuals. Bach, Delrieu, and Delbarre (1970a, b) described circulating human lymphocytes which bear surface receptors with antiglobulin activity. Upon mixing and centrifuging with human erythrocytes coated with rabbit antibody, each lymphocyte was surrounded by adherent erythrocytes to form a rosette; usually fewer than six rosetteforming lymphocytes per thousand were found in control subjects, whereas over 70 per cent of patients with rheumatoid arthritis gave greater numbers. Patients with gout gave increased rosette counts. These authors suggested that the lymphocyte response of the rheumatoid individual precedes the appearance of humoral rheumatoid factors and that the process may correspond to that found in animals after immunization (Brown and Epstein, 1969).In order to examine the properties and occurrence of rosette-forming lymphocytes, we have tested lymphocyte preparations from normal subjects of various ages, patients suffering from rheumatoid arthritis, and patients with orthopaedic conditions not related to rheumatoid arthritis. Material and methodsThe ability of human lymphocyte preparations to form rosettes was measured by allowing a mixture of washed lymphocytes and antibody-coated erythrocytes to sediment gently (Lamvik and Godal, 1970), re-suspending, and examining in wet film by darkground illumination.Lymphocytes were isolated from fresh heparinized blood either directly or after enrichment by sedimentation of erythrocytes using Plasmagel (Tosi, Pellegrino, Scudeller, and Ceppellini, 1967). Density centrifugation by the method of Froland and Natvig (1970) gave generally low Accepted for publication October 12, 1971. yields of lymphoyctes, but although the method did not give reproducibly less than 1-5 per cent. granulocytes, contamination was usually quite small. Lymphocytes isolated by this method were washed three times in Hanks's or Eagle's medium, re-suspended in the residual supematant, and adjusted to 8,000 cell/mm3.Human OD+ erythrocytes were sensitized with immune Ripley anti-CD serum (kindly supplied by Dr. Marion Waller) for 1 hr at 37°C. (Waller and Vaughan, 1956), washed once, and then used immediately at 1 per cent. concentration. Sheep erythrocytes were sensitized with glycerinated rabbit amboceptor serum (Wellcome) by keeping at room temperature for 30 minutes or more a mixture containing 1 per cent. cells and a suitable concentration of serum. To avoid possible agglutination, this cell suspension was not centrifuged before use. Unlike Bach and others (1971) we used a dose of rabbit amboceptor which gave optimum sensitization for the Waaler-Rose test as determined with a reference panel of sera. This antiserum concentration was unrelated to the h...

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