Influence of Honeybee Sting on Peptidome Profile in Human Serum

Matysiak, Jan; Światły, Agata; Hajduk, Joanna; Matysiak, Joanna; Kokot, Zenon J.

doi:10.3390/toxins7051808

Cited by 7 publications

(11 citation statements)

References 32 publications

(36 reference statements)

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“…Generation of a QC model is based on storing the class averages of the peak areas together with p-values obtained from a t -test. First, the peak areas or intensities are sorted per peak and next a weighted average from all peaks need to be calculated [ 59 , 61 ]. SNN algorithm uses a prototype-based classification.…”

Section: Methodsmentioning

confidence: 99%

“…The characteristic spectra of each study class, which are named prototypes, are identified. Then, they are considered as prototypical samples of that class from a proteomic point of view [ 59 , 61 ]. Two indicators of the model’s performance, 20% leave one out cross validation (10 iterations) and recognition capability, were calculated for all three used algorithms.…”

Section: Methodsmentioning

confidence: 99%

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Identification of Serum Peptidome Signatures of Non-Small Cell Lung Cancer

Klupczynska

Światły

Hajduk

et al. 2016

IJMS

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Due to high mortality rates of lung cancer, there is a need for identification of new, clinically useful markers, which improve detection of this tumor in early stage of disease. In the current study, serum peptide profiling was evaluated as a diagnostic tool for non-small cell lung cancer patients. The combination of the ZipTip technology with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for the analysis of peptide pattern of cancer patients (n = 153) and control subjects (n = 63) was presented for the first time. Based on the observed significant differences between cancer patients and control subjects, the classification model was created, which allowed for accurate group discrimination. The model turned out to be robust enough to discriminate a new validation set of samples with satisfactory sensitivity and specificity. Two peptides from the diagnostic pattern for non-small cell lung cancer (NSCLC) were identified as fragments of C3 and fibrinogen α chain. Since ELISA test did not confirm significant differences in the expression of complement component C3, further study will involve a quantitative approach to prove clinical utility of the other proteins from the proposed multi-peptide cancer signature.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Identification of Serum Peptidome Signatures of Non-Small Cell Lung Cancer

Klupczynska

Światły

Hajduk

et al. 2016

IJMS

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“…apaminę, która jest neurotoksyną, peptydy niszczące (degranulujące) komórki tuczne, wazoaktywne aminy oraz białka enzymatyczne, np. hialuronidazę czy fosfolipazę (18). Melityna ma właściwości hemolityczne.…”

Section: Discussionunclassified

Clinical and behavioral aspects of bee stings in domestic dog

Karpinski¹,

Goleman²,

Czyzowski³

et al. 2016

Medycyna Weterynaryjna

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The aim of this study was to determine the scale of the phenomenon and the risks resulting from stings of domestic dog by bees. The material consisted of clinical interviews on dog disease history from four veterinary clin-ics. Twenty-six cases of stings were selected, in which the dog owner could positively identify the insect species that had stung a dog (on the basis of the external appearance of a dead bee or the presence of its sting). The select-ed dogs were grouped by sex and age: juveniles – aged up to 12 months and adults – aged more than 12 months. In addition, the grouping took into account the occurrence of pulmonary edema symptoms or lack thereof and the body response. Three types of body response were distinguished: a local reaction (M) in the form of erythema with edema, a large local reaction (MD), and anaphylaxis (pulmonary edema confirmed by X-ray examination). The following blood counts were evaluated: erythrocytes, leukocytes, lymphocytes, hematocrit value, hemoglobin con-centration, segmented neutrophils percentage, MCV, MCH, MCHC, as well as biochemical indices: AP, ALT, AST, creatinine, and BUN. Clinical and behavioral symptoms were analyzed with the use of a survey conducted with a randomly selected group of dog owners. To illustrate the scale of the problem, the survey on bee stings was conducted among 97 dog owners. An analysis of the coefficients of correlation between the values of the above parameters and the age of stung dogs showed that with dog’s age, there was a decrease in AP, lymphocyte and leukocyte levels, and an increase in hemoglobin levels percentage of segmented neutrophils, as well as hematocrit and erythrocyte counts. A statistically significant differ-ence was ascertained in AST, whose values were significantly higher in dogs without pulmonary edema (33.6 U/l ± 14.0) than in the group with edema (22.7 U/l ± 4.6), t (22) = 2.856; p = 0.01. A comparison of the average values of the parameters under analysis for both sexes revealed a significant difference between the average values of hemo-globin, which was significantly higher in males (16.6 g/dl ± 2.6), compared to females (14.1 g/dl ± 2.6), t (24) = 2,156; p = 0.041. The dogs were also classified into two groups according to the type of reaction: a local reaction or a large local reaction. Significantly greater differences were found in the average content of erythrocytes in dogs with a local reaction. Among all respondents, 35% reported their dogs having been stung by insects from the family Hymenoptera. Male dogs were stung twice as often as females, and almost half of the sting cases were dogs older than 4 years. Most of the stung dogs were kept in the backyard, and purebred dogs were stung more frequently than crossbreeds. Half of all cases were stings on the head, and the stung dogs usually reacted by strong hyperkinesia and slight vocalization (one point on a three-point scale).

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“…Serum peptides can be used to distinguish a normal, healthy individual from someone who has recently been stung by a wasp [19]. After analyzing the serum levels of 34 amino acids, Matysiak et al demonstrated that the levels of L-glutamine (Gln), L-glutamic acid (Glu), L-methionine (Met), and 3-methyl-L-histidine (3MHis) were significantly different between wasp sting victims and healthy controls [20].…”

Section: Introductionmentioning

confidence: 99%

Serum Metabolomics Analysis in Wasp Sting Patients

Yang

Chai

Liu

et al. 2018

BioMed Research International

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To analyze the dynamic changes of serum metabolomics in wasp sting victims, we collected serum from 10 healthy volunteers and 10 patients who had been stung 3 hours, 24 hours, and 72 hours before sample collection. We analyzed the metabolomics by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) techniques and then performed enrichment analysis. A total of 838 metabolites were identified. Serum metabolomics analysis using MetaboAnalyst revealed 289 metabolites that were significantly different among patients in the 3-hour group versus healthy controls (P<0.001). Pathway analysis of those metabolites indicated that those metabolic sets were associated with sphingolipid metabolism. Based on the differences among the control, 3-hour, 24-hour, and 72-hour groups, we classified serum metabolites into different categories. The first and second categories included 297 and 280 metabolites that were significantly different in terms of concentration among healthy controls versus the participants whose sera were analyzed 3 hours, 24 hours, and 72 hours after wasp stings. Pathway analysis of those metabolites indicated that those metabolic sets were associated with thiamine metabolism. The third category included 269 significant metabolites. The fourth category included 28 significant metabolites. Pathway analysis of the metabolites in third and fourth categories indicated that those metabolic sets were associated with phenylalanine, tyrosine, and tryptophan biosynthesis. The fifth category included 31 metabolites, which were not significantly different between the control and 3-hour groups but were higher in concentration in the 24-hour and 72-hour groups. Pathway analysis of the fifth category of significant metabolites identified linoleic acid metabolism. In conclusion, multiple metabolic pathways are associated with wasp stings, and these might provide a basis for exploring mechanisms of wasp sting injury and potential targets for therapy.

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Influence of Honeybee Sting on Peptidome Profile in Human Serum

Cited by 7 publications

References 32 publications

Identification of Serum Peptidome Signatures of Non-Small Cell Lung Cancer

Identification of Serum Peptidome Signatures of Non-Small Cell Lung Cancer

Clinical and behavioral aspects of bee stings in domestic dog

Serum Metabolomics Analysis in Wasp Sting Patients

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