Influence of High Concentrations of Copper Sulfate on In Vitro Adventitious Organogenesis of Cucumis sativus L.

2022

Preprint

Self Cite

The effect of different concentrations of sodium salicylate (NaSA), a phenolic compound, on in vitro callus and shoot regeneration of cucumber (Cucumis sativus L.) was investigated. Four-day-old cotyledon explants from the Spanish cultivar 'Marketer' were employed. MS-derived shoot induction medium containing 0.5 mg L-1 IAA and 2.5 mg L-1 BAP was supplemented with NaSA. Frequency and shoot number were enhanced by 1.5-fold with NaSA at 10-7M. Higher salicylate levels led to increased callus formation and decreased shoot regeneration. The application of sodium salicylate at a specific concentration showed a positive trend in in vitro adventitious organogenesis of a commercial cucumber cultivar. Some probable mechanisms that may underlie the beneficial effects of salicylic acid/salicylates on in vitro regeneration were also discussed.

Section: Plant Materials and In Vitro Regenerationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of Sodium Salicylate on Adventitious Organogenesis of a Commercial Cucumber Cultivar

2022

Preprint

Self Cite

“…Peterson) and of the cultivars 'Marketer' and 'Negrito' (Semillas Fitó S.A.), were the starting material. Obtaining axenic explants and in vitro adventitious regeneration were based on the methodology previously described by Miguel (2021) in cucumber, with some modifications.…”

Section: Plant Materials and Regenerationmentioning

confidence: 99%

“…World production of cucumber, including gherkins, ranked in 2019 third among vegetable crops with 87,805,086 tonnes, and a harvested area of 2,231,402 hectares (FAOSTAT, http://www.fao.org/faostat/en/#data/QC). Crop improvement of cucumber for traits that confer resistance/tolerance to major biotic/abiotic stresses is difficult through conventional breeding due to its narrow genetic base, low genetic variability, and various crossing barriers with related species (Den Nijs and Custers 1990;Plader et al 2007;Miguel 2021). Besides, conventional approaches are labor-intensive, timeconsuming, and costly.…”

Section: Introductionmentioning

confidence: 99%

“…Besides, conventional approaches are labor-intensive, timeconsuming, and costly. Genetic engineering and plant transformation techniques have the potential to overcome these constraints (Miguel 2021). Despite the number of The optimal lighting conditions depend on species, cultivars, plant growth stages, specific secondary metabolites, and other environmental parameters, such as nutrients, temperature, and CO2 levels (Dou and Niu 2020).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Light Conditions onIn VitroAdventitious Organogenesis of Cucumber Cultivars

2021

Preprint

Self Cite

The response on callus and shoot formation under different light incubation conditions was evaluated in cucumber (Cucumis sativus L.). Four-day-old cotyledon explants from the inbred line ‘Wisconsin 2843’ and the commercial cultivars ‘Marketer’ and ‘Negrito’ were employed. A four-week culture was conducted on MS-derived shoot induction medium containing 0.5 mg L-1 IAA and 2.5 mg L-1 BAP, under an 8-h dark/ 16-h light regime, or by a one- or two-week dark pre-incubation followed by the same photoperiod. Significant differences were obtained for the regeneration of shoots in all cultivars. The response in both frequency and number of shoots under continuous photoperiod was at least 3-6 fold higher than with dark pre-incubation. The highest genotypes response was obtained by ‘Negrito’ and ‘Marketer’ with identical values. All explants formed callus, and in two of the three cultivars, the response on callus extension was not significantly affected by incubation conditions. The results clearly show that shoot induction under continuous photoperiod regime was beneficial for adventitious shoot regeneration in cucumber.

Influence of sodium salicylate on adventitious organogenesis of a commercial cucumber cultivar

2023

MSJ

Bhutia et al 2018). SA application has been reported to modulate plant functions dose-dependently (Maurya et al 2019). Inadequate levels of SA can also lead to oxidative stress (Gondor et al 2016). SA-like effects have been reported with structurally similar compounds, such as NaSA and acetylsalicylic acid (ASA). Some authors have previously described improved in vitro plant regeneration with exogenous SA or ASA. In the present work, it is investigated by applying NaSA.The cucumber (Cucumis sativus L.) is an economically important species belonging to the Cucurbitaceae family, along with melon, watermelon, and squash. Its world production, including gherkins, ranked third among vegetable crops in 2020, Abstract Cucumber is an important vegetable crop belonging to the Cucurbitaceae family. An optimized regeneration system is required to address different biotechnological approaches in this species, such as large-scale multiplication, as well as genome editing and genetic transformation techniques. The aim of this study was to investigate the effect of different concentrations of the phenolic compound sodium salicylate (NaSA), a salicylic acid derivative, on in vitro callus and shoot regeneration of a commercial cultivar of cucumber. Four-day-old cotyledon explants from the slicing cultivar 'Marketer' were employed. Murashige and Skoog (MS)-derived shoot induction medium containing 0.5 mg L-1 indole-3acetic acid (IAA) and 2.5 mg L-1 6-benzylaminopurine (BAP) was supplemented with NaSA. Frequency and shoot number were enhanced by 1.5-fold with NaSA at 0.1 µM. Higher salicylate levels led to increased callus formation and decreased shoot regeneration. The application of sodium salicylate at a specific concentration showed a positive trend in in vitro adventitious organogenesis of a commercial cucumber cultivar. Some probable mechanisms that may underlie the beneficial effects of salicylic acid/salicylates on in vitro regeneration were also discussed.