Influence of heterotrophic and mixotrophic cultivation on growth behaviour of terrestrial cyanobacteria

Schwarz, Anna; Walther, Jakob; Geib, Doris; Witthohn, Marco; Strieth, Dorina; Ulber, Roland; Muffler, Kai

doi:10.1016/j.algal.2020.102125

Cited by 9 publications

(2 citation statements)

References 24 publications

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“…Similar observations for Chlorella vulgaris were noted during transcriptomic studies of the transition of photoautotrophic to mixotrophic metabolism, in which biomass after the addition of glucose started an exponential phase [22]. Additionally, the studies of terrestrial cyanobacteria cells under heterotrophic and mixotrophic conditions did not show a lag phase [28]. The lag phase for C. sorokiniana during mixotrophic cultivation with different volatile fatty acids lasted less than 1 day, which can also be the explanation for observations in the present study because the measurements were taken once a day [29].…”

Section: Resultssupporting

confidence: 79%

Application of Extracellular Polymeric Substances during Cultivation of Microalgae Biomass

Rusanowska,

Zieliński,

Dudek

et al. 2023

Applied Sciences

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Extracellular polymeric substances (EPS) produced by microorganisms contain polymers that are used for the bioflocculation of microalgae; however, these polymers are also organic compounds that might be used as carbon sources. The study analyzed two strategies for the introduction of EPS for Tetraselmis subcordiformis, Chlorella sp., and Arthrospira platensis biomass harvesting. In the first variant, EPS in the dose of 100 mg TOC/g were added to the photobioreactor every other day from the beginning of the cultivation, while in the second variant, EPS in the two doses of 100 mg TOC/g and 300 mg TOC/g were only added at the end of cultivation. In the first variant, the results proved that microalgae/cyanobacteria can use the EPS as external carbon sources. The cultures were characterized by a faster increase in biomass concentration, which contained less chlorophyll. However, the EPS content did not change. In the second variant, the addition of EPS did not affect the EPS content and the sedimentation of the Chlorella sp. biomass. The biomass of T. subcordiformis was characterized by a much better sedimentation coefficient. The greatest differences were observed in the A. platensis culture: the biomass concentration increased from 1.2 ± 0.2 g/L to 1.9 ± 0.2 g/L, EPS content increased by 16%, and sedimentation efficiency increased to 72%.

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Section: Resultssupporting

confidence: 79%

Application of Extracellular Polymeric Substances during Cultivation of Microalgae Biomass

Rusanowska,

Zieliński,

Dudek

et al. 2023

Applied Sciences

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“…PCC 6803 (hereafter Synechocystis ) [ 63 ], can also effectively utilize glucose (Glc) from the extracellular medium, and thereby simultaneously use photosynthesis and glycolysis to grow mixotrophically. Mixotrophy typically gives the cells an advantage as seen in enhanced growth [ 49 ] and increased productivity of certain chemical compounds [ 32 , 38 , 60 ].…”

Section: Introductionmentioning

confidence: 99%

Interplay between photosynthetic electron flux and organic carbon sinks in sucrose-excreting Synechocystis sp. PCC 6803 revealed by omics approaches

Muth-Pawlak,

Kakko,

Kallio

et al. 2024

Microb Cell Fact

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Background Advancing the engineering of photosynthesis-based prokaryotic cell factories is important for sustainable chemical production and requires a deep understanding of the interplay between bioenergetic and metabolic pathways. Rearrangements in photosynthetic electron flow to increase the efficient use of the light energy for carbon fixation must be balanced with a strong carbon sink to avoid photoinhibition. In the cyanobacterium Synechocystis sp. PCC 6803, the flavodiiron protein Flv3 functions as an alternative electron acceptor of photosystem I and represents an interesting engineering target for reorganizing electron flow in attempts to enhance photosynthetic CO2 fixation and increase production yield. Results We have shown that inactivation of Flv3 in engineered sucrose-excreting Synechocystis (S02:Δflv3) induces a transition from photoautotrophic sucrose production to mixotrophic growth sustained by sucrose re-uptake and the formation of intracellular carbon sinks such as glycogen and polyhydroxybutyrate. The growth of S02:Δflv3 exceeds that of the sucrose-producing strain (S02) and demonstrates unforeseen proteomic and metabolomic changes over the course of the nine-day cultivation. In the absence of Flv3, a down-regulation of proteins related to photosynthetic light reactions and CO2 assimilation occurred concomitantly with up-regulation of those related to glycolytic pathways, before any differences in sucrose production between S02 and S02:Δflv3 strains were observed. Over time, increased sucrose degradation in S02:Δflv3 led to the upregulation of respiratory pathway components, such as the plastoquinone reductase complexes NDH-11 and NDH-2 and the terminal respiratory oxidases Cyd and Cox, which transfer electrons to O2. While glycolytic metabolism is significantly up-regulated in S02:Δflv3 to provide energy for the cell, the accumulation of intracellular storage compounds and the increase in respiration serve as indirect sinks for photosynthetic electrons. Conclusions Our results show that the presence of strong carbon sink in the engineered sucrose-producing Synechocystis S02 strain, operating under high light, high CO2 and salt stress, cannot compensate for the lack of Flv3 by directly balancing the light transducing source and carbon fixing sink reactions. Instead, the cells immediately sense the imbalance, leading to extensive reprogramming of cellular bioenergetic, metabolic and ion transport pathways that favor mixotrophic growth rather than enhancing photoautotrophic sucrose production.

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Sustainable Production of Pigments from Cyanobacteria

Deepika

Wolf

Roles

et al. 2022

Advances in Biochemical Engineering/Biotechnology

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Influence of heterotrophic and mixotrophic cultivation on growth behaviour of terrestrial cyanobacteria

Cited by 9 publications

References 24 publications

Application of Extracellular Polymeric Substances during Cultivation of Microalgae Biomass

Application of Extracellular Polymeric Substances during Cultivation of Microalgae Biomass

Interplay between photosynthetic electron flux and organic carbon sinks in sucrose-excreting Synechocystis sp. PCC 6803 revealed by omics approaches

Sustainable Production of Pigments from Cyanobacteria

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