Influence of hematocrit on quantitative analysis of "blood spots" on filter paper

O’Broin, S.

doi:10.1093/clinchem/39.6.1354

Cited by 36 publications

(18 citation statements)

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“…Contrarily, examination of published data reveals, in some cases, that biases are particularly pronounced at high Hct levels, as observed for naproxen (on Whatman FTA and FTA DMPK‐C) (Youhnovski et al, ) and for NIM811, a cyclophilin inhibitor (on Whatman FTA) (Li et al, ). On the other hand, and similar to 51 Cr (O'Broin, ), some amino acids (Holub et al, ) and drugs (Denniff & Spooner, ; Xu et al, ) did not yield significant variations according to the Hct level.…”

Section: Blood Collectionmentioning

confidence: 67%

“…Hct is defined as the volume fraction (expressed as a percentage) of blood occupied by erythrocytes and has been investigated early as a parameter that could impact quantitative results (O'Broin, ; O'Broin, Kelleher, & Gunter, ). Hct varies with age, gender, health status, and slightly with ethnicity.…”

Section: Blood Collectionmentioning

confidence: 99%

“…Originally, a first strategy to estimate blood volume contained in a punch relied on the use of a chemical tracer. Examples include 125 I‐human serum albumin ( 125 I‐hSA), 51 Cr (O'Broin, ), Hb (O'Broin, Kelleher, & Gunter, ), or 125 I‐L‐thyroxin (Adam et al, ). Actually, estimated volumes are more often only representative of one blood fraction (e.g., erythrocytes or plasma), and were shown to significantly differ depending on the tracer.…”

Section: Blood Collectionmentioning

confidence: 99%

“…As for chromatographic effects, one aspect to consider is the partition of the analyte between the erythrocyte and the plasma fraction. Based on 51 Cr measurements, it has been suggested that analytes partitioned in erythrocytes may be less sensitive to Hct effects (O'Broin, ). As mentioned before, preferential partitioning in erythrocytes concerns several drugs and notably some immunosuppressants (i.e., cyclosporine A, tacrolimus, everolimus, sirolimus) (Hinderling, ).…”

Section: Blood Collectionmentioning

confidence: 99%

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The use of mass spectrometry to analyze dried blood spots

Wagner

Tonoli

Varesio

et al. 2014

Mass Spectrometry Reviews

209

217

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Dried blood spots (DBS) typically consist in the deposition of small volumes of capillary blood onto dedicated paper cards. Comparatively to whole blood or plasma samples, their benefits rely in the fact that sample collection is easier and that logistic aspects related to sample storage and shipment can be relatively limited, respectively, without the need of a refrigerator or dry ice. Originally, this approach has been developed in the sixties to support the analysis of phenylalanine for the detection of phenylketonuria in newborns using bacterial inhibition test. In the nineties tandem mass spectrometry was established as the detection technique for phenylalanine and tyrosine. DBS became rapidly recognized for their clinical value: they were widely implemented in pediatric settings with mass spectrometric detection, and were closely associated to the debut of newborn screening (NBS) programs, as a part of public health policies. Since then, sample collection on paper cards has been explored with various analytical techniques in other areas more or less successfully regarding large‐scale applications. Moreover, in the last 5 years a regain of interest for DBS was observed and originated from the bioanalytical community to support drug development (e.g., PK studies) or therapeutic drug monitoring mainly. Those recent applications were essentially driven by improved sensitivity of triple quadrupole mass spectrometers. This review presents an overall view of all instrumental and methodological developments for DBS analysis with mass spectrometric detection, with and without separation techniques. A general introduction to DBS will describe their advantages and historical aspects of their emergence. A second section will focus on blood collection, with a strong emphasis on specific parameters that can impact quantitative analysis, including chromatographic effects, hematocrit effects, blood effects, and analyte stability. A third part of the review is dedicated to sample preparation and will consider off‐line and on‐line extractions; in particular, instrumental designs that have been developed so far for DBS extraction will be detailed. Flow injection analysis and applications will be discussed in section IV. The application of surface analysis mass spectrometry (DESI, paper spray, DART, APTDCI, MALDI, LDTD‐APCI, and ICP) to DBS is described in section V, while applications based on separation techniques (e.g., liquid or gas chromatography) are presented in section VI. To conclude this review, the current status of DBS analysis is summarized, and future perspectives are provided. © 2014 Wiley Periodicals, Inc. Mass Spec Rev 35:361–438, 2016.

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Section: Blood Collectionmentioning

confidence: 67%

Section: Blood Collectionmentioning

confidence: 99%

Section: Blood Collectionmentioning

confidence: 99%

Section: Blood Collectionmentioning

confidence: 99%

See 2 more Smart Citations

The use of mass spectrometry to analyze dried blood spots

Wagner

Tonoli

Varesio

et al. 2014

Mass Spectrometry Reviews

209

217

View full text Add to dashboard Cite

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“…Hematocrit (Hct) which is a measure of the proportions of blood that constitutes RBC has the potential to affect drug concentration when DBS sampling is adopted 173…”

Section: Hematocritmentioning

confidence: 99%

Evaluation of paper spray mass spectrometry for direct analysis of small molecule drugs and hemoglobin constant spring peptide markers

Takyi-Williams¹

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Effects of spot size on biomarker levels of field‐collected dried blood spots: A new algorithm for exact dried blood spot size measurement

Groh

Weiss

Börsch‐Supan³

et al. 2022

American J Hum Biol

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Objectives: The quality of blood values analyzed from survey-collected dried blood spot (DBS) samples is affected by fieldwork conditions, particularly spot size. We offer an image-based algorithm that accurately measures the area of field-collected DBS and we investigate the impact of spot size on the analyzed blood marker values.Methods: SHARE, a pan-European study, collected 24 000 DBS samples in 12 countries in its sixth wave. Our new algorithm uses photographs of the DBS samples to calculate the number of pixels of the blood-covered area to measure the spot sizes accurately. We ran regression models to examine the association of spot size and seven DBS analytes. We then compared the application of our new spot-size measures to common spot-size estimation. Results: Using automated spot-size measurement, we found that spot size has a significant effect on all markers. Smaller spots are associated with lower measured levels, except for HbA1c, for which we observe a negative effect. Our precisely measured spot sizes explain substantially more variance of DBS analytes compared to commonly used spot-size estimation. Conclusion:The new algorithm accurately measures the size of field-collected DBS in an automated way. This methodology can be applied to surveys even with very large numbers of observations. The measured spot sizes improve the accuracy of conversion formulae that translate blood marker values derived from DBS into venous blood values. The significance of the spot-size effects on biomarkers in DBS should also incentivize the improvement of fieldwork training and monitoring.

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Influence of hematocrit on quantitative analysis of "blood spots" on filter paper

Cited by 36 publications

References 0 publications

The use of mass spectrometry to analyze dried blood spots

The use of mass spectrometry to analyze dried blood spots

Evaluation of paper spray mass spectrometry for direct analysis of small molecule drugs and hemoglobin constant spring peptide markers

Effects of spot size on biomarker levels of field‐collected dried blood spots: A new algorithm for exact dried blood spot size measurement

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