Influence of Helicobacter pylori on platelets after bone marrow transplantation from unrelated donors

Wakae, Takeshi; Takatsuka, Hiroyuki; Mori, Akira; Okada, Masaya; Fujimori, Yoshihiro; Okamoto, Takahiro; Hara, Hiroshi; Kakishita, Eizo

doi:10.1038/sj.bmt.1703884

Cited by 4 publications

(4 citation statements)

References 22 publications

(16 reference statements)

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“…However, in a study of Chinese carriers of H. pylori during allogeneic bone marrow transplantation, a positive association between H. pylori carriage and reduced risk of gut acute GVHD was demonstrated 1 . Although a previous study has shown that H. pylori infection accelerates recovery of the platelet count after HSCT, possibly by stimulating IL-6 production 20 , we have not identified any association between platelet, leukocyte and neutrophil counts and the presence of H. pylori in the oral mucosa.…”

Section: Discussioncontrasting

confidence: 41%

“…In addition, as a recent study demonstrated a reduced incidence of acute GVHD of the gut in Chinese carriers of H. pylori during allogeneic bone transplantation 1 , we studied the effect of the presence of H. pylori on the severity of oral GVHD. Finally, as H. pylori has an influence on the platelet count in HSCT patients 20 , the association between platelets number and the presence of H. pylori was also studied.…”

Section: Introductionmentioning

confidence: 99%

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Helicobacter pylori in the oral mucosa of patients submitted to allogeneic haematopoietic stem cell transplantation

et al. 2006

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ABSTRACT:This study was designed to investigate the impact of haematopoietic stem cell transplantation (HSCT) on Helicobacter pylori colonization of the oral mucosa by nested polymerase chain reaction (nested-PCR). Forty six consecutive patients submitted to HSCT and 46 healthy volunteers were included in the study. Oral swabs were taken from the oral mucosa of the patients and control group. The medical records of the patients were reviewed and the following information was retrieved: gender and age of the patient, donor gender, primary disease, stem cell source (bone marrow or blood stem cells), leukocyte, neutrophil and platelet counts, and chronic graft versus host disease (cGVHD) of salivary glands. The results demonstrated an increased frequency of H. pylori in the oral mucosa of HSCT patients compared to controls (ρ = 0.002). The presence of H. pylori in the oral mucosa was not related to the severity of cGVHD. The median counts of platelet/mm 3 , leukocytes/mm 3 and neutrophils/mm 3 in the group of HSCT patients positive for H. pylori were not statistically different from those of the patients negative for it. In conclusion, the present study shows increased frequency of H. pylori in the oral mucosa of HSCT patients compared to non-transplanted healthy volunteers. DESCRIPTORS:Helicobacter pylori; Bone marrow transplantation; Mouth mucosa. RESUMO:O objetivo do estudo é investigar o impacto do transplante de células-tronco hematopoiéticas (TCTH) na colonização da mucosa bucal pela Helicobacter pylori através do "nested-PCR". Quarenta e seis pacientes submetidos ao TCTH e 46 indivíduos saudáveis foram incluídos no estudo. Raspados de mucosa bucal foram realizados nos pacientes do grupo de estudo e grupo controle. Os dados médicos dos pacientes foram revisados e as seguintes informações foram coletadas: gênero e idade do paciente, gênero do doador, doença primária, fonte de células-tronco (medula óssea ou células-tronco sanguíneas), número de leucócitos, neutrófi los e plaquetas, doença do enxerto contra o hospedeiro crônica (DECHc) de glândulas salivares. Os resultados demonstram aumento na freqüência de H. pylori na mucosa bucal de pacientes submetidos ao TCTH comparado com grupo controle (ρ = 0.002). A presença da H. pylori na mucosa bucal não teve relação com a severidade da DECHc. As medianas de número de plaquetas/mm 3 , leucócitos/mm 3 e neutrófi los/mm 3 no grupo de pacientes TCTH positivos para H. pylori não foram estatisticamente diferentes das medianas dos pacientes negativos. Concluindo, o presente estudo mostra um aumento da freqüência da H. pylori na mucosa bucal de pacientes submetidos ao TCTH quando comparada com a de um grupo de voluntários não transplantados saudáveis.

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Section: Discussioncontrasting

confidence: 41%

Section: Introductionmentioning

confidence: 99%

Helicobacter pylori in the oral mucosa of patients submitted to allogeneic haematopoietic stem cell transplantation

et al. 2006

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“…Can pathogens present in one strain, but absent in another, bias published conclusions on the role of a specific receptor or ligand? To date, no information is available, but it is worth acknowledging the studies on another Helicobacter species, Helicobacter pylori, and a growing awareness for its ability to influence aspects of platelet function (62,63).…”

Section: Resultsmentioning

confidence: 99%

Dysfunctional platelet membrane receptors: from humans to mice

Ware

2004

Thromb Haemost

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SummaryInsights into hemostasis and thrombosis have historically benefited from the astute diagnosis of human bleeding and thrombotic disorders followed by decades of careful biochemical characterization. This work has set the stage for the development of a number of mouse models of hemostasis and thrombosis generated by gene targeting strategies in the mouse genome. The utility of these models is the in depth analysis that can be performed on the precise molecular interactions that support hemostasis and thrombosis along with efficacy testing of various therapeutic strategies. Already the mouse has proven to be an excellent model of the processes that support hemostasis and thrombosis in the human vasculature. A brief summary of the salient phenotypes from knockout mice missing key platelet receptors is presented, including the glycoprotein (GP) Ib-IX-V and GP IIb/IIIa (αIIb/β3) receptors; the collagen receptors, GPVI and α2βI; the protease activated receptors (PARs); and the purinergic receptors, P2Y 1 and P2Y 2 . A few differences exist between mouse and human platelets and where appropriate those will be highlighted in this review. Concluding remarks focus on the importance of understanding the power and limitations of various in vitro, ex vivo and in vivo models currently being used and the impact of the mouse strain on the described platelet phenotype.

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“…Concerning the effect of H. pylori infection on platelets, Wakae et al reported rapid recovery in peripheral platelet counts after the bone marrow transplantation among H. pylori-positive patients. Platelet counts of H. pylori-positive patients reached 20 Â 10 9 /l 16.4þ/À2.7 days after bone marrow transplantation, whereas the negative patients required 32.4þ/À13.3 days [11]. Recently, Ozel et al reported lower levels of IL-6 in H. pylori-negative patients and its decline after eradication of H. pylori in patients with Familial Mediterranean Fever [12].…”

Section: Discussionmentioning

confidence: 96%

Long term changes in platelet counts afterH. pylorieradication in non-ITP patients

Matsukawa

Iwamoto²,

Kato³

et al. 2010

Platelets

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Helicobacter pylori eradication is becoming a first-line therapy against idiopathic thrombocytopenic purpura (ITP) and its long term efficacy has already been reported. In contrast, eradication therapy reduced peripheral platelets in non-ITP patients 8 weeks later. To confirm the long term efficacy of Helicobacter eradication on platelet counts in non-ITP patients, we evaluated changes in peripheral platelet counts in endoscopically diagnosed patients with Helicobacter infection. Endoscopically diagnosed patients with Helicobacter infection received eradication therapy using amoxicillin (1500 mg/day), clarithromycin (400 mg/day) and lansoprazole (60 mg/day). The changes in platelet counts after Helicobacter eradication were serially evaluated for as long as 3 years or more. In total, 294 patients were enrolled: 243 patients successfully received eradication therapy and 51 were unsuccessfully treated. As a whole, peripheral platelet counts significantly decreased after Helicobacter eradication, being reduced by more than 1.0 × 10⁹/l by 5-6 months, 1 year, 2 years and 3 years or more (from 24.2+/-5.6 to 23.1+/-5.0, 23.0+/-5.0, 22.1+/-4.5, 22.4+/-5.6, and 21.6+/-5.3 × 10⁹/l: p = <0.0001, <0.0001, 0.0001, 0.0052, and <0.0001, respectively). Helicobacter pylori eradication finally reduced peripheral platelet counts around 2.0 × 10⁹/l in non-ITP patients. There was a definite difference in platelet regulation by Helicobacter pylori between ITP and non-ITP patients. These bivalent effects, upregulation and downregulation, on the peripheral platelet induced by Helicibacter pylori infection appeared to originate from quite different mechanisms.

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Influence of Helicobacter pylori on platelets after bone marrow transplantation from unrelated donors

Cited by 4 publications

References 22 publications

Helicobacter pylori in the oral mucosa of patients submitted to allogeneic haematopoietic stem cell transplantation

Helicobacter pylori in the oral mucosa of patients submitted to allogeneic haematopoietic stem cell transplantation

Dysfunctional platelet membrane receptors: from humans to mice

Long term changes in platelet counts afterH. pylorieradication in non-ITP patients

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