Influence of Heating Regimes on Dimensional and Colorimetric Changes of Teeth

Sandholzer, Michael A.

doi:10.1016/b978-0-12-800451-7.00021-8

Cited by 10 publications

(8 citation statements)

References 36 publications

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“…Despite the visual observation of teeth heated for 15-minutes and 30-minutes being different, our spectrophotometric results were generally unable to find a significant effect of duration on colour variables or crystal size (p < 0.05). This finding is in stark contrast with a previous study in which duration of heat-exposure was shown to affect tooth colour changes (21) . Our results also showed that the lightness, L* value, is not affected by temperature.…”

Section: Spectrophotometric Analysiscontrasting

confidence: 99%

“…As the intensity of heating increases, tooth colour progressively changes. In general, the sequence of colour changes is from its neutral colour (yellowish white), to brown, black, blue-grey, and finally chalky white (8,20,21).…”

Section: Colour Analysismentioning

confidence: 99%

“…More generally, the interpretation of colour based on visual observation is subjective because this method entirely depends on an individual's perception (19,21). Due to this subjectivity, the ability of individuals to describe colour is inconsistent from time-to-time (23).…”

Section: Colour Analysismentioning

confidence: 99%

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Integrating spectrophotometric and XRD analyses in the investigation of burned dental remains

Rahmat

Humphries

Austin

et al. 2020

Forensic Science International

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Heat alters colour and crystallinity of teeth by destruction of the organic content and inducing hydroxyapatite crystal growth. The colour and crystallite changes can be quantified using spectrophotometric and x-ray diffraction analyses, however these analyses are not commonly used in combination to evaluate burned dental remains. In this study, thirty-nine teeth were incinerated at 300 -1000°C for 15 and 30 minutes and then measured using a spectrophotometer and an x-ray diffractometer. Response variables used were lightness, L*, and chromaticity a* and b* and luminance (whiteness and yellowness) for colour, and crystal size for crystallinity. Statistical analysis to determine the attribution of these variables revealed yellowness and crystal size were significantly affected by temperature (p < 0.05), whilst duration of heat-exposure showed no significant effect. This study suggests the inclusion of both spectrophotometric and x-ray diffraction in investigating thermal-heated teeth is useful to accurately estimate the temperature teeth are exposed to.

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Section: Spectrophotometric Analysiscontrasting

confidence: 99%

Section: Colour Analysismentioning

confidence: 99%

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Integrating spectrophotometric and XRD analyses in the investigation of burned dental remains

Rahmat

Humphries

Austin

et al. 2020

Forensic Science International

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“…Calcination of the surface of crown and root, inner layers (dentin, pulp chamber and root canal) showed a lower degree of alteration by the fire, due to the protection of the more affected outer layers (Supplementary material TableS1 and TableS2) [16]. These color changes observed in the studied teeth are similar in order of appearance of those observed in bone [13,25]. However, we have to take into account that this study was done in nude teeth, normally maxillary and mandibular bones as well as facial tissues protect them from external insults, thus the results can be slightly different at least in the first exposed temperatures.…”

Section: Macroscopic Changesmentioning

confidence: 55%

Evaluation of macroscopic changes and the efficiency of DNA profiling from burnt teeth

2016

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Identification of human remains subjected to incineration is extremely challenging. Our study evaluates the macroscopic changes and efficiency of DNA profiling in burnt teeth under controlled temperature and time conditions. 28 teeth were exposed to temperatures between 100 and 700°C for a duration of 1-15min. Two non-burnt teeth were used as control. Macroscopic changes were evaluated and recorded. DNA was extracted using a silica-based methodology. Efficiency of DNA profiling was assessed through Quantitative PCR for STRs. Burnt teeth reached chalky white appearance at 400°C 5min and fractures were observed from 300°C 10min. Amplification of STRs was very low from 300°C and 1 or 5min. In contrast, the housekeeping gene, GAPDH, was amplified in all combinations of temperatures and times. Although it is possible to amplify the housekeeping gene at high temperature, DNA profiling is difficult to obtain, probably due to small size of these regions making them more prone to degradation.

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“…Methods compiled by Adams and Byrd () leaned more toward forensic applications, but chapters on ethics (Egana, Turner, Doretti, Bernardi, & Ginarte, ), osteometric sorting (Byrd, ; Byrd & LeGarde, ), X‐ray fluorescence sorting (Perrone, Finlayson, Bartelink, & Dalton, ), epiphyseal sequencing (Schaefer, ), estimating the most likely number of individuals (MLNI) (Adams & Konigsberg, ; Konigsberg & Adams, ), spatial analysis (Herrmann & Devlin, ; Tuller & Hofmeister, ), and recovery methods (Naji et al, ) were of clear utility for bioarchaeologists in the analysis of broken, mixed remains. Schmidt and Symes’ (2015) volume on burned human bone offered commingling‐related methods for fire‐modified remains such as the Bab adh‐Dhra’ EB II–III collection, discussing bone and tooth color changes (Beach, Passalacqua, & Chapman, ; Devlin & Herrmann, ; Ullinger & Sheridan, ), thermal breakdown of bone (DeHaan, ; Schurr, Hayes, & Cook, ; Thompson, ), enamel changes (Mahoney & Miszkiewicz, ; Sandholzer, ; Schmidt, ), and site‐specific reconstructions (Curtin, ; McKinley, ; Schmidt et al, ; Wahl, ). Haglund and Sorg's older edited volumes (1997, 2002) provided several taphonomic considerations for studies of commingling (Correia, ; Darwent & Lyman, ; Galloway, ; Haglund, Connor, & Scott, ; Lyman & Fox, ; Roksandic, ; Saul and Saul, ; Ubelaker, ).…”

Section: Commingling Challengesmentioning

confidence: 99%

Bioarchaeology in the ancientNearEast: Challenges and future directions for the southern Levant

Sheridan

2017

American J Phys Anthropol

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The synthesis of biological anthropology, archaeology, and social theory provides a bioarchaeological model to reconstruct nuanced aspects of demography, diet, disease, death, daily activities, and biodistance, even in the absence of discrete burials. Numerous skeletal assemblages in the southern Levant are composed of mixed and fragmented bones resulting from generational use of cemeteries, mass burial, and additional communal burial practices. Others become commingled due to taphonomic processes such as flooding, geological events, or human mediated mechanisms like looting, improper excavation, and poor curation. Such collections require one to ask broader questions of human adaptability, exercise a holistic approach, use broad demographic categories, and remain cognizant of the limitations posed by fragmentation. Expanded research questions and ethical considerations, the use of centralized databases and understudied collections, as well as the application of social media, citizen science, and crowd sourcing provide new tools for bioarchaeological analyses of the many commingled ancient Near Eastern collections in the southern Levant.

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Influence of Heating Regimes on Dimensional and Colorimetric Changes of Teeth

Cited by 10 publications

References 36 publications

Integrating spectrophotometric and XRD analyses in the investigation of burned dental remains

Integrating spectrophotometric and XRD analyses in the investigation of burned dental remains

Evaluation of macroscopic changes and the efficiency of DNA profiling from burnt teeth

Bioarchaeology in the ancientNearEast: Challenges and future directions for the southern Levant

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