Influence of heat treatment on rabbit liver ferritin

Frenkel, Erik J.; Beld, Bep van den; Oost, B.A. van; Marx, Joannes J.M.

doi:10.1016/0167-4838(83)90050-x

Cited by 14 publications

(2 citation statements)

References 22 publications

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“…This may be due to the purification process. The high yield we have obtained may be due to the lower loss of protein achieved by our procedure since multiple steps used in the isolation and purification of ferritin cause high losses of the protein (Frenkel et al ., 1983;Cham et al ., 1985Cham et al ., , 1986.…”

Section: Discussionmentioning

confidence: 94%

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Partial purification and characterization of ferritin from the liver and intestinal mucosa of chickens, turtledoves and mynahs

et al. 2005

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Ferritin is the iron-storage protein responsible for sequestering excess iron, to be stored in a safe way in the liver or to be shed with the intestinal epithelial cells. The properties of ferritin in iron-overload-susceptible birds have not been elucidated. Furthermore, there is only scarce information on mucosal ferritin, with no information at all in avian species. Here we have studied the liver and proximal intestine ferritins of iron-overload-susceptible (Indian hill mynahs, common mynahs) and non-susceptible (turtledoves, chicken) bird species. A brief purification process preceded native polyacrylamide gel electrophoresis and staining the gels for protein and iron. Protein amounts and iron-binding characteristics of ferritin were measured and ferritin saturation levels were calculated. Although ferritin protein amounts did not differ significantly, liver and mucosal ferritins of sensitive bird species incorporated much more iron, leading to high saturation levels. Significantly higher ferritin iron content and saturation were observed in the liver of both mynah species and in the intestinal ferritin of Indian hill mynahs when compared with the non-susceptible species. Ferritin appears not to play a major role in the regulation of iron absorption, implicating other phases in iron transport to be more important in the onset and process of iron overload in birds.

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Section: Discussionmentioning

confidence: 94%

“…One millitre of each homogenate was subjected to heat treatment for 10 min at 758C to aid the isolation of ferritin since other proteins are not stable at that temperature (Frenkel et al ., 1983). After heat treatment the samples were immediately cooled down on ice for 30 min.…”

Section: Methodsmentioning

confidence: 99%