Influence of heat stress to matrix on bone formation

Yoshida, Keiko; Uoshima, Katsumi; Oda, Kimimitsu; Maeda, Takeyasu

doi:10.1111/j.1600-0501.2009.01654.x

Cited by 55 publications

(37 citation statements)

References 30 publications

(30 reference statements)

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“…Low-level heating in the range of 40-42°C using various heating devices (e.g., water bath, humidified incubator, heating blanket, and temperature stimulator) induced positive in vitro and in vivo responses for tissue regeneration in skin (Harder et al 2004), muscle (Riederer et al 2008), cartilage (Hojo et al 2003), teeth (Lee et al 2008), heart (Wang et al 2008;Rylander et al 2005), and bone (Shui and Scutt 2001;Yoshida et al 2009). Even though it is difficult to accurately compare the results of each study due to the variation in heating protocols employed and type of cells considered, utilization of lowlevel heating is preferred for skeletal tissue studies due to its ability to increase metabolic activity and cellular proliferation without cytotoxicity (Hojo et al 2003;Shui and Scutt 2001;Ye et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Response of preosteoblasts to thermal stress conditioning and osteoinductive growth factors

Chung

Rylander

2012

Cell Stress and Chaperones

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Conditioning protocols involving mechanical stress independently or with chemical cues such as growth factors (GFs) possess significant potential to enhance bone regeneration. However, utilization of thermal stress conditioning alone or with GFs for bone therapy has been under-investigated. In this study, a preosteoblast cell line (MC3T3-E1) was exposed to treatment with water bath heating (44°C, 4 and 8 min) and osteoinductive GFs (bone morphogenetic protein-2 and transforming growth factor-β1) individually or in combination to investigate whether these stimuli could promote induction of bone-related markers, an angiogenic factor, and heat shock proteins (HSPs). Cells remained viable when heating durations were less than 20 min at 40ºC, 16 min at 42ºC, and 10 min at 44ºC. Increasing heating duration at 44°C, promoted gene expression of HSPs, osteocalcin (OCN), and osteopontin (OPN) at 8 h post-heating (PH). Heating in combination with GFs caused the greatest gene induction of osteoprotegerin (OPG; 6.9- and 1.6-fold induction compared to sham-treated and GF only treated groups, respectively) and vascular endothelial growth factor (VEGF; 16.0- and 1.6-fold compared to sham and GF-only treated groups, respectively) at 8 h PH. Both heating and GFs independently suppressed the matrix metalloproteinase-9 (MMP-9) gene. GF treatment caused a more significant decrease in MMP-9 protein secretion to non-detectable levels compared to heating alone at 72 h PH. Secretion of OCN, OPN, and OPG increased with the addition of GFs but diminished with heating as measured by ELISA at 72 h PH. These results suggest that conditioning protocols utilizing heating and GFs individually or in combination can induce HSPs, bone-related proteins, and VEGF while also causing downregulation of osteoclastic activity, potentially providing a promising bone therapeutic strategy.

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Section: Introductionmentioning

confidence: 99%

Response of preosteoblasts to thermal stress conditioning and osteoinductive growth factors

Chung

Rylander

2012

Cell Stress and Chaperones

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“…For example, a mere periosteum reflection results in osteocyte cell death through the lacuna‐canalicular network, which may be observed as blank lacunae 10. In our histological images, a thicker blank lacunae area was observed with the stainless steel cavity than the zirconia cavity.…”

Section: Discussionmentioning

confidence: 65%

Biological Evaluation of Implant Drill Made from Zirconium Dioxide

Akiba

Eguchi

Akiba

et al. 2016

Clin Implant Dent Rel Res

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PurposeZirconia is a good candidate material in the dental field. In this study, we evaluated biological responses against a zirconia drill using a bone cavity healing model.Materials and MethodsZirconia drills, stainless steel drills, and the drilled bone surface were observed by scanning electron microscopy (SEM), before and after cavity preparation. For the bone cavity healing model, the upper first and second molars of Wistar rats were extracted. After 4 weeks, cavities were prepared with zirconia drills on the left side. As a control, a stainless steel drill was used on the right side. At 3, 7, and 14 days after surgery, micro‐CT images were taken. Samples were prepared for histological staining.ResultsSEM images revealed that zirconia drills maintained sharpness even after 30 drilling procedures. The bone surface was smoother with the zirconia drill. Micro‐CT images showed faster and earlier bone healing in the zirconia drill cavity. On H‐E staining, at 7 days, the zirconia drill defect had a smaller blank lacunae area. At 14 days, the zirconia drill defect was filled with newly formed bone.ConclusionsThe zirconia drill induces less damage during cavity preparation and is advantageous for bone healing. (197 words)

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“…Nevertheless, some previous studies showed that HS induces a positive effect on bone growth and remodeling in vitro and in vivo. 13,33 Recently, conditions involving heating and growth factors in combination resulted in the inhibition of osteoclast differentiation and bone-resorptive processes in the bone microenvironment. 32 The close relationship between HSP synthesis and tissue regeneration under HS conditions has also been reported.…”

Section: Et Almentioning

confidence: 99%

Mild Heat Stress Enhances Angiogenesis in a Co-culture System Consisting of Primary Human Osteoblasts and Outgrowth Endothelial Cells

Fuchs²,

Böse³

et al. 2014

Tissue Engineering Part C: Methods

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The repair and regeneration of large bone defects, including the formation of functional vasculature, represents a highly challenging task for tissue engineering and regenerative medicine. Recent studies have shown that vascularization and ossification can be stimulated by mild heat stress (MHS), which would offer the option to enhance the bone regeneration process by relatively simple means. However, the mechanisms of MHS-enhanced angiogenesis and osteogenesis, as well as potential risks for the treated cells are unclear. We have investigated the direct effect of MHS on angiogenesis and osteogenesis in a co-culture system of human outgrowth endothelial cells (OECs) and primary osteoblasts (pOBs), and assessed cytotoxic effects, as well as the levels of various heat shock proteins (HSPs) synthesized under these conditions. Enhanced formation of microvessel-like structures was observed in co-cultures exposed to MHS (41°C, 1 h), twice per week, over a time period of 7-14 days. As shown by real-time polymerase chain reaction (PCR), the expression of vascular endothelial growth factor (VEGF), angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2), and tumor necrosis factor-alpha was up-regulated in MHS-treated co-cultures 24 h post-treatment. At the protein level, significantly elevated VEGF and Ang-1 concentrations were observed in MHS-treated co-cultures and pOB mono-cultures compared with controls, indicating paracrine effects associated with MHS-induced angiogenesis. MHS-stimulated co-cultures and OEC mono-cultures released higher levels of Ang-2 than untreated cultures. On the other hand MHS treatment of cocultures did not result in a clear effect regarding osteogenesis. Nevertheless, real-time PCR demonstrated that MHS increased the expression of mitogen-activated protein kinase, interleukin-6, and bone morphogenetic protein 2, known as HSP-related molecules in angiogenic and osteogenic regulation pathways. In agreement with these observations, the expression of some selected HSPs also increased at both the mRNA and protein levels in MHS-treated co-cultures.

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Influence of heat stress to matrix on bone formation

Abstract: Although there was a temperature-dependent delay in bone formation after heat stress, the 48 degrees C heat stress did not obstruct bone formation eventually. This delay was probably caused by slow periosteal membrane regeneration.

Cited by 55 publications

References 30 publications

Response of preosteoblasts to thermal stress conditioning and osteoinductive growth factors

Response of preosteoblasts to thermal stress conditioning and osteoinductive growth factors

Biological Evaluation of Implant Drill Made from Zirconium Dioxide

Mild Heat Stress Enhances Angiogenesis in a Co-culture System Consisting of Primary Human Osteoblasts and Outgrowth Endothelial Cells

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