Influence of GSTs, CYP2E1 and mEH polymorphisms on 1, 3-butadiene-induced micronucleus frequency in Chinese workers

Tan, Haidong; Wang, Qi; Wang, Aihong; Ye, Yunjie; Feng, Na; Feng, Xingyu; Lu, Lingeng; Au, William W.; Zheng, Yuxin; Zhang, Xia

doi:10.1016/j.taap.2010.07.006

Cited by 11 publications

(6 citation statements)

References 32 publications

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“…In recent years, cytokinesis-block micronucleus cytome (CBMN Cyt) assay has evolved into a comprehensive method for measuring chromosome breakage, DNA misrepair, chromosome loss, nondisjunction, necrosis, apoptosis, and cytostasis [ 21 ]. Using CBMN cytome assay, our study shows that BD exposure could induce chromosomal damage (MNi and NPBs) in BD-exposed workers, which was consistent with two other studies in China [ 10 , 16 ]. However, several studies have yielded negative results [ 6 – 9 ].…”

Section: Discussionsupporting

confidence: 91%

“…Thus, the polymorphisms of metabolic genes ( CYP2E1 , GST s, and mEH ) involved in BD metabolism were included in several studies to understand their relationship with BD genotoxicity. Epidemiological studies on BD-exposed workers indicated that many polymorphic loci of metabolic genes can impact the chromosomal damage induced by BD exposure [ 13 – 16 ]. In 2009, using cytokinesis-blocked micronucleus (CBMN) cytome assay, we found that BD-exposed workers exhibited increased frequencies of micronuclei (MNi) and nucleoplasmic bridges (NPBs) when compared to subjects in the control group.…”

Section: Introductionmentioning

confidence: 99%

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Association between Genetic Polymorphisms of DNA Repair Genes and Chromosomal Damage for 1,3-Butadiene-Exposed Workers in a Matched Study in China

Xiang

Sun

Dong

et al. 2015

BioMed Research International

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The aim of the study was to examine the association between polymorphisms of DNA repair genes and chromosomal damage of 1,3-butadiene- (BD-) exposed workers. The study was conducted in 45 pairs of occupationally exposed workers in a BD product workshop and matched control workers in an administrative office and a circulatory water workshop in China. Newly developed biomarkers (micronuclei, MNi; nucleoplasmic bridges, NPBs; nuclear buds, NBUDs) in the cytokinesis-blocked micronucleus (CBMN) cytome assay were adopted to detect chromosomal damage. PCR and PCR-restriction fragment length polymorphism (RFLP) are adopted to analyze polymorphisms of DNA repair genes, such as X-ray repair cross-complementing Group 1 (XRCC1), O6-methylguanine-DNA methyltransferase (MGMT), poly (adenosine diphosphate-ribose) polymerases (ADPRT), and apurinic/apyrimidinic endonucleases (APE1). The BD-exposed workers exhibited increased frequencies of MNi and NPBs when compared to subjects in the control group. The results also show that the BD-exposed workers carrying XRCC1 diplotypes TCGA-CCGG (4.25 ± 2.06‰) (FR = 2.10, 95% CI: 1.03–4.28) and TCGG-TCGA (5.80 ± 3.56‰) (FR = 2.75, 95% CI: 0.76–2.65) had statistically higher NBUD frequencies than those who carried diplotype TCGG-TCGG (1.89 ± 1.27‰). Our study suggests that polymorphisms of XRCC1 gene may influence chromosomal damage in BD-exposed workers.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Association between Genetic Polymorphisms of DNA Repair Genes and Chromosomal Damage for 1,3-Butadiene-Exposed Workers in a Matched Study in China

Xiang

Sun

Dong

et al. 2015

BioMed Research International

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“…BD-exposed workers who possessed the GSTT1 null genotypes showed significantly higher frequencies of chromosomal aberrations than the appropriate controls. Tan concluded that BD-exposed workers carrying the GSTM1 (−) genotype had a higher MN frequency than those carrying GSTM1 (+); no difference was found between the GSTT1 genotype and MN frequency [28].…”

Section: Discussionmentioning

confidence: 97%

“…Several reports have documented chromosome damage in workers exposed to BD [1,27]. Tan considered that BD exposure would increase the frequency of MN [28]; however, these results were not supported by findings of a lack of increased genetic damage in 1,3-butadiene-exposed workers in studies conducted by Zhang [29]. Our results showed that BD exposed workers had a significantly higher MN frequency than controls, and this was especially true for those exposed to higher cumulative BD levels, which was consistent with previous research.…”

Section: Discussionmentioning

confidence: 99%

The association between genetic damage in peripheral blood lymphocytes and polymorphisms of three glutathione S-transferases in Chinese workers exposed to 1,3-butadiene

Cheng

Zhang

Zhao

et al. 2013

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…The Xia group in the School of Public Health of Fudan University used the cytokinesis-block micronucleus (CBMN) assay to examine chromosomal damage in peripheral blood lymphocytes (PBL) of 166 workers in a polybutadiene latex chemical industrial plant in Ningbo, China [ 163 ]. These workers were exposed to extremely high levels of BD with the range varying from 0.05 to 1,985.99 mg/m 3 (the median was 4.48 mg/m 3 ).…”

Section: Introductionmentioning

confidence: 99%

1,3-Butadiene: a ubiquitous environmental mutagen and its associations with diseases

Chen

Zhang

2022

Genes and Environ

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Abstract1,3-Butadiene (BD) is a petrochemical manufactured in high volumes. It is a human carcinogen and can induce lymphohematopoietic cancers, particularly leukemia, in occupationally-exposed workers. BD is an air pollutant with the major environmental sources being automobile exhaust and tobacco smoke. It is one of the major constituents and is considered the most carcinogenic compound in cigarette smoke. The BD concentrations in urban areas usually vary between 0.01 and 3.3 μg/m3 but can be significantly higher in some microenvironments. For BD exposure of the general population, microenvironments, particularly indoor microenvironments, are the primary determinant and environmental tobacco smoke is the main contributor. BD has high cancer risk and has been ranked the second or the third in the environmental pollutants monitored in most urban areas, with the cancer risks exceeding 10-5. Mutagenicity/carcinogenicity of BD is mediated by its genotoxic metabolites but the specific metabolite(s) responsible for the effects in humans have not been determined. BD can be bioactivated to yield three mutagenic epoxide metabolites by cytochrome P450 enzymes, or potentially be biotransformed into a mutagenic chlorohydrin by myeloperoxidase, a peroxidase almost specifically present in neutrophils and monocytes. Several urinary BD biomarkers have been developed, among which N-acetyl-S-(4-hydroxy-2-buten-1-yl)-L-cysteine is the most sensitive and is suitable for biomonitoring BD exposure in the general population. Exposure to BD has been associated with leukemia, cardiovascular disease, and possibly reproductive effects, and may be associated with several cancers, autism, and asthma in children. Collectively, BD is a ubiquitous pollutant that has been associated with a range of adverse health effects and diseases with children being a subpopulation with potentially greater susceptibility. Its adverse effects on human health may have been underestimated and more studies are needed.

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Influence of GSTs, CYP2E1 and mEH polymorphisms on 1, 3-butadiene-induced micronucleus frequency in Chinese workers

Cited by 11 publications

References 32 publications

Association between Genetic Polymorphisms of DNA Repair Genes and Chromosomal Damage for 1,3-Butadiene-Exposed Workers in a Matched Study in China

Association between Genetic Polymorphisms of DNA Repair Genes and Chromosomal Damage for 1,3-Butadiene-Exposed Workers in a Matched Study in China

The association between genetic damage in peripheral blood lymphocytes and polymorphisms of three glutathione S-transferases in Chinese workers exposed to 1,3-butadiene

1,3-Butadiene: a ubiquitous environmental mutagen and its associations with diseases

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