Influence of GSTM1 and GSTT1 genotypes and confounding factors on the frequency of sister chromatid exchange and micronucleus among road construction workers

Kumar, Anil; Yadav, Anita; Giri, Shiv Kumar; Dev, Kapil; Gautam, Sanjeev K.; Gupta, Ranjan; Aggarwal, Neeraj

doi:10.1016/j.chemosphere.2011.04.008

Cited by 8 publications

(8 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a study of Tuimala et al (2004), GSTT1-null subjects showed a statistically significant increase in the frequency of baseline CAs in an unexposed Caucasian population. An altered MN frequency has also been associated with occupationally or environmentally exposed GSTT1-null individuals previously (Laffon et al, 2006;Chen et al, 2010;Kumar et al, 2011).Our results regarding the frequencies of the studied GST polymorphisms are consistent with those in previous Turkish studies ( Kocabas et al, 2000;Ada et al, 2004Ada et al, , 2007. The GSTM1-null genotype is very common among Caucasians, being found in roughly 50% of the population (Hirvonen, 1999).…”

supporting

confidence: 82%

Section: Susceptibility To Dna Damage In a Turkish Population 1159mentioning

confidence: 99%

See 1 more Smart Citation

Assessment of Individual Susceptibility to Baseline DNA and Cytogenetic Damage in a Healthy Turkish Population: Evaluation with Lifestyle Factors

Kadıoğlu

Kocabaş

Demircigil

et al. 2012

Genetic Testing and Molecular Biomarkers

View full text Add to dashboard Cite

Background: Cytogenetic biomarkers are most frequently used well-established endpoints in human population studies with their sensitivity for measuring exposure to genotoxic agents. They have an important role as early predictors of cancer risk. Identification of individual genotypes of metabolic gene polymorphisms helps to understand the modulation of cancer susceptibility by environmental exposures, such as cigarette smoking and other lifestyle factors. Aim: To evaluate individual susceptibility to chemicals, we determined individual DNA damage related to glutathione S-transferase (GST) genotypes (GSTM1, GSTT1, and GSTP1) in a Turkish population. Methods: Peripheral blood lymphocytes (PBL) and DNA samples of 127 subjects were analyzed for the presence of DNA damage, using single-cell gel electrophoresis (the Comet assay), and for cytogenetic parameters (chromosomal aberrations [CAs], bleomycin-induced CA, and a cytokinesis-blocked micronucleus assay), and the polymerase chain reaction/restriction fragment length polymorphism method, respectively. Results: Individuals carrying a GSTT1-null allele showed higher frequencies of CA and micronucleus (MN) ( p = 0.026, p = 0.003, respectively), whereas the GSTM1-null and GSTP1 mutant genotypes did not show any differences in cytogenetic parameters. Our findings demonstrated that none of the lifestyle factors (smoking, alcohol drinking, dietary habits, vitamin intake, and physical activity), except for vitamin intake ( p = 0.002), were significantly associated with the studied cytogenetic parameters. Conclusion: Our results suggest that the GSTT1 gene polymorphism may influence the baseline cytogenetic frequency in a healthy population. IntroductionT he role of genetic factors (early predictors) in determining human susceptibility to the carcinogenic effects of chemical agents has become a major research area in molecular epidemiology (Au, 2007). Biomarkers of early biologic effect include cytogenetic assays, such as chromosomal aberrations (CAs), micronucleus (MN), and the comet assay, which are the most frequently used endpoints in human population studies. Their sensitivity for measuring exposure to genotoxic agents and their role as early predictors of cancer risk have contributed to this success (Bonassi et al., 2005). CAs and cytokinesis-blocked micronucleus assay (CBMN) have value as biological dosimeters, and more recently, as predictors of cancer risk in epidemiological studies (Bonassi et al., 2004(Bonassi et al., , 2011. Mechanistic evidence linking CAs to early stages of cancer has been supported by the results of cohort studies that associated the CA frequency in peripheral lymphocytes of healthy individuals to future cancer incidence (Bonassi et al., 2008). Furthermore, it has been found that an increased MN frequency in peripheral blood lymphocytes predicts the risk of cancer in humans (Bonassi et al., 2007). The alkaline comet assay is also one of the most promising biomarkers to detect single-strand breaks (SSBs) as well as alkaline-labile sites under ...

show abstract

supporting

confidence: 82%

Section: Susceptibility To Dna Damage In a Turkish Population 1159mentioning

confidence: 99%

Assessment of Individual Susceptibility to Baseline DNA and Cytogenetic Damage in a Healthy Turkish Population: Evaluation with Lifestyle Factors

Kadıoğlu

Kocabaş

Demircigil

et al. 2012

Genetic Testing and Molecular Biomarkers

View full text Add to dashboard Cite

show abstract

“…As compared to other cytogenetic techniques, the cytokinesis-blocked micronucleus (CBMN) assay is a rapid and simple technique to detect the cytogenetic damage in the populations exposed to radiation, pesticides and genotoxic chemicals (Bolognesi et al, 1993;2002;Scarpato et al, 1996;Da Silva Augusto et al, 1997;Joksic et al, 1997;Meng and Zhang, 1997;Calvert et al, 1998;Gómez-Arroyo et al, 2000;Pastor et al, 2001;Costa et al, 2006;Bhalli et al, 2006;Kumar et al, 2011;Sellappa et al, 2010;2011 a, b;Balamuralikrishnan et al, 2012;Erdem et al, 2012;Donmez-Altuntas and Bitgen, 2012;Gajski et al, 2013;Rodrigues et al, 2014;Yang et al, 2014). An increased MN frequency in the circulating lymphocytes indicates increased cancer risk for those exposed to genotoxic agents (Znaor et al, 2003;Bonassi et al, 2007;.…”

Section: Introductionmentioning

confidence: 99%

Increased Micronucleus Frequency in Peripheral Blood Lymphocytes Contributes to Cancer Risk in the Methyl Isocyanate-Affected Population of Bhopal

Senthilkumar¹,

Akhter²,

Malla³

et al. 2015

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

The Bhopal gas tragedy involving methyl isocyanate (MIC) is one of the most horrific industrial accidents in recent decades. We investigated the genotoxic effects of MIC in long-term survivors and their offspring born after the 1984 occurrence. There are a few cytogenetic reports showing genetic damage in the MIC-exposed survivors, but there is no information about the associated cancer risk. The same is true about offspring. For the first time, we here assessed the micronucleus (MN) frequency using cytokinesis-blocked micronucleus (CBMN) assay to predict cancer risk in the MIC-affected population of Bhopal. A total of 92 healthy volunteers (46 MICaffected and 46 controls) from Bhopal and various regions of India were studied taking gender and age into consideration. Binucleated lymphocytes with micronuclei (BNMN), total number of micronuclei in lymphocytes (MNL), and nuclear division index (NDI) frequencies and their relationship to age, gender and several lifestyle variabilities (smoking, alcohol consumption and tobacco-chewing) were investigated. Our observations showed relatively higher BNMN and MNL (P<0.05) in the MIC-affected than in the controls. Exposed females (EF) exhibited significantly higher BNMN and MNL (P<0.01) than their unexposed counterparts. Similarly, female offspring of the exposed (FOE) also suffered higher BNMN and MNL (P<0.05) than in controls. A significant reduction in NDI (P<0.05) was found only in EF. The affected group of non-smokers and non-alcoholics featured a higher frequency of BNMN and MNL than the control group of non-smokers and non-alcoholics (P<0.01). Similarly, the affected group of tobacco chewers showed significantly higher BNMN and MNL (P<0.001) than the non-chewers. Amongst the affected, smoking and alcohol consumption were not associated with statistically significant differences in BNMN, MNL and NDI. Nevertheless, tobacco-chewing had a preponderant effect with respect to MNL. A reasonable correlation between MNL and lifestyle habits (smoking, alcohol consumption and tobacco-chewing) was observed only in the controls. Our results suggest that EF and FOE are more susceptible to cancer development, as compared to EM and MOE. The genotoxic outcome detected in FOE reflects their parental exposure to MIC. Briefly, the observed cytogenetic damage to the MIC-affected could contribute to cancer risk, especially in the EF and FOE.

show abstract

“…On the other hand, the same author (VAN POPPEL et al, 1994) analyzed the MN frequency in sputum cells of smokers and concluded that this frequency was not influenced by GSTM1. More recent studies on occupationally or environmentally exposed individuals showed that higher MN frequency was associated with GSTT1 null genotype (LAFFON et al, 2006;KUMAR et al, 2011). However, KIRSCH-VOLDERS et al (2006) reported lower MN frequency in the occupationally exposed individuals with GSTT1 and GSTM1 null genotypes.…”

Section: Resultsmentioning

confidence: 98%

“…Table 5. Results of multiple regression analysis for citokinesis-block proliferation index (CBPI) including GSTT1 and GSTM1 status and potential modifiers of CBPI DISCUSSION Numerous literature data suggested importance of GST genes in defense against endogenously agents (CHEN et al, 2010;KUMAR et al, 2011) and that their mutations could associate with different diseases (MANFREDI et al, 2007;MUSTAFA et al, 2010) especially with cancer (ATES et al, 2005;HO et al, 2006;SINGH et al, 2008).…”

Section: Statistical Analysesmentioning

confidence: 99%

Influence of GSTT1 and GSTM1 null genotypes on differentiated thyroid cancer risk and baseline and radioiodine induced cytogenetic damage in peripheral blood lymphocytes of patients

et al. 2017

View full text Add to dashboard Cite

show abstract

Influence of GSTM1 and GSTT1 genotypes and confounding factors on the frequency of sister chromatid exchange and micronucleus among road construction workers

Cited by 8 publications

References 53 publications

Assessment of Individual Susceptibility to Baseline DNA and Cytogenetic Damage in a Healthy Turkish Population: Evaluation with Lifestyle Factors

Assessment of Individual Susceptibility to Baseline DNA and Cytogenetic Damage in a Healthy Turkish Population: Evaluation with Lifestyle Factors

Increased Micronucleus Frequency in Peripheral Blood Lymphocytes Contributes to Cancer Risk in the Methyl Isocyanate-Affected Population of Bhopal

Influence of GSTT1 and GSTM1 null genotypes on differentiated thyroid cancer risk and baseline and radioiodine induced cytogenetic damage in peripheral blood lymphocytes of patients

Contact Info

Product

Resources

About