Influence of growth conditions on the production of extracellular proteolytic enzymes in Paenibacillus peoriae NRRL BD-62 and Paenibacillus polymyxa SCE2

Abstract

Aims: To analyse the extracellular protease profile of two Paenibacillus species, Paenibacillus peoriae and Paenibacillus polymyxa, as well as how different growth media influenced its expression. Methods and Results: Both bacteria were cultured in five media [Luria–Bertani broth, glucose broth, thiamine/biotin/nitrogen broth (TBN), trypticase soy broth and a defined medium] for 48 h at 32°C. Our results showed a heterogeneous protease secretion pattern whose expression was dependent on medium composition. However, TBN induced the most quantitative and qualitative protease production on both Paenibacillus. The proteases were detected in neutral‐alkaline pH range, being totally inhibited by 1,10‐phenanthroline, a zinc‐metalloprotease inhibitor. We also analysed the protease expression during the growth and, at least to P. peoriae, the most elevated protease activity was measured at 96 h, in which the highest number of spores and a low concentration of viable cells were observed. Conclusions: The results presented add P. peoriae and P. polymyxa to the list of neutral‐alkaline extracellular protease producers. Significance and Impact of the Study: Paenibacillus species are ubiquitous in nature, are capable to form resistant spores and to produce several hydrolytic enzymes, including proteases. However, only few data concerning the production of these enzymes are available. Proteases produced by Paenibacillus strains may represent new sources for biotechnological use.

Ecology and biotechnological potential of Paenibacillus polymyxa: a minireview

“…Neutral-alkaline proteases constitute a very large and complex group of enzymes, with both nutritional and regulatory roles in nature. The major applications of these enzymes are in detergent formulation, food industry, leather processing, chemical synthesis and waste management [54] (Table 1). Ishii et al [55] have reported the production of fl avin reductase from P. polymyxa A-l that couples effi ciently with desulfurizing enzymes (DszA and DszC).…”

Draft Genome Sequence of Paenibacillus peoriae Strain KCTC 3763T

“…With the exception of antimicrobial activity against plant pathogens (10) and the production of hydrolytic enzymes that may be beneficial to crop plants (1), there are few reports regarding the utility of P. peoriae. While analyzing the genome sequence of P. polymyxa E681, a potent plant growthpromoting strain isolated from the rhizosphere of barley (5), we found that the 16S rRNA gene sequence is much closer to that of P. peoriae.…”

Enzymes Produced by Halotolerant Spore-Forming Gram-Positive Bacterial Strains Isolated From a Resting Habitat (Restinga de Jurubatiba) in Rio de Janeiro, Brazil: Focus on Proteases

“…The molecular masses of the proteases were calculated by comparison with the mobility of low molecular mass standards. The gels were dried, scanned, and digitally processed [19].…”

“…blutaparonensis M9 strain was cultivated in LB broth at 32°C for 48 h. After that, the culture (400 mL) was centrifuged (15,000×g/20 min/4°C) and the supernatant was filtered through a 0.22-μm membrane (Millipore). The cell-free culture supernatant was concentrated tenfold using a 10,000 molecular weight cut-off Amicon micropartition system (Stirred Cell Model 8200) [19]. Protein concentration was determined by the method described by Lowry et al [20], using bovine serum albumin (BSA) as standard.…”