Research background. Dhaincha (Sesbania aculeate) is a forage legume which is primarily used for green manuring and animal feeding. Good nutritional profile of dhaincha makes it a potential alternative legume in human nutrition. However, the presence of high amount of antinutrients pose problem in its utilization for food applications. The present investigation was intended to germinate dhaincha seeds at different time-temperature regimes and to evaluate the dynamics of characteristics of germinating dhaincha to ascertain optimal germination conditions to improve its potential for utilization.
Experimental approach. Dhaincha seeds were germinated at 24, 28 and 32 °C for 24, 48 and 72 h. Germinating dhaincha was evaluated for its germination characteristics and losses, spectral characteristics, techno-functionality, antinutrients, bioactive constituents, antioxidant capacity and mineral elements. Optimal balance of techno-biofunctionality of germinating dhaincha seeds was validated by principal component analysis.
Results and conclusions. Sprout length and germination losses increased with the higher germination temperature and prolonged germination time. Seeds showed similar germination rate at 28 and 32 °C and it was markedly higher in contrast to germination at 24 °C Germination for 24 h resulted in mild conformational changes in the secondary structure of protein whereas germination for 48 and 72 h exhibited major conformational changes in the β-sheets, resulting in consequent improvement in the hydration and foaming properties. Progression of germination (72 h) was manifested with the reduction of tannins (24.47 %), phytic acid (16.38 %), saponins (24.58 %), trypsin inhibitor (40.33 %) and lectin activity (62.50 %). Slight reduction was observed for results of DPPH∙ (3.7%) and ABTS∙+ (18.5%) assays, whereas total flavonoids (36.14 %) and metal chelating activity (26.76 %) increased. Total phenolics, FRAP, and reducing power exhibited decline after 24 h followed by a gradual increase. Zinc extractability increased drastically by germination. Germination at 28 °C for 72 h manifested higher reduction of antinutrients with comparatively less compromise on antioxidant activity and better functional characteristics as validated by principal component analysis.
Novelty and scientific contribution. Dhaincha is an unknown crop in Europe and even in Asia it is predominantly used as green manure and animal feed. This research demonstrated that that intervention of germination can transform dhaincha into a promising crop for food industry. Germinated dhaincha exhibited enhanced techno-biofunctionality for utilization in various food formulations.