Influence of glutathione S-transferase polymorphisms on genotoxic effects induced by tobacco smoke

Palma, Selena; Cornetta, Tommaso; Padua, Luca; Cozzi, Renata; Appolloni, Massimo; Ievoli, Elena; Testa, Antonella

doi:10.1016/j.mrgentox.2007.03.014

Cited by 45 publications

(20 citation statements)

References 61 publications

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“…[38][39][40][41] In a multivariate logistic analysis, we have evidenced that the GSTT1/GSTM1*0 ([ ]/ [-]) combined genotype provides a greater susceptibility for the development of spinocellular carcinoma of head and neck, similar results to those found by other authors. 7,23,42,43 Various studies could not correlate the null GSTT1 genotype with the head and neck cancer, 26,27,43 which was a result found in our study. Similarly, there was no association between this type of neoplasia and the null combined genotypes null GSTT1 and null GSTM1, as observed by Singh et al 7 On the other hand, a Chinese study has shown a correlation between the double null genotype GSTT1 and GSTM1 with nasopharynx cancer among men.…”

Section: Discussioncontrasting

confidence: 74%

Análise dos genes GSTM1 e GSTT1 em pacientes com câncer de cabeça e pescoço

Leme¹,

Raposo²,

Ruiz³

et al. 2010

Rev. Assoc. Med. Bras.

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Objective. To establish the clinical and demographic profile and identify risk factors among patients with head and neck cancer, relating them to the polymorphism of GSTT1 and GSTM1. MethOds. One hundred patients with head and neck cancer and 100 control group individuals without history of neoplasm were analyzed. The molecular analysis was made by multiplex polymerase chain reaction. For statistical analysis, data were tabulated and compared by the Fisher's exact test, the Chi-square test and multiple logistic regression were also used.Results. There was prevalence of smokers (OR = 5.32, CI 95% CI = 2.04-13.86 p = 0.0006), alcohol drinkers (OR = 5.04, CI 95% = 2.19-11.59 p = 0.0001) in head and neck cancer patients. The GSTT1 null genotype was found in 47% of the patient and 41% of the control group (OR = 0.67; CI 95%= 0.34-1.35; p = 0.2648). Likewise, the GSTM1 null genotype was found in 66% of the patient and 75% of the control group (OR = 2.25; CI 95%= 1.05 -4.84; p = 0.0368). The combined GSTT1 and GSTM1 gene null genotype shown association between GSTM1*0/GSTT1 and occurrence of head and neck carcinoma (OR = 7.64; CI 95%= 1.72-34.04; p = 0.0076). Analysis of clinical-pathological features showed association between GSTT1 null genotype and larynx, the inverse relation between this genotype and pharynx. cOnclusiOn. In our study it was possible to establish an association between the nullity of GSTM1 genotype and that of combined GSTT1/GSTM1*O ([]/ [-] genotypes and head and neck cancer. gstM1 and gstt1 genes analysis in head and neck canceR patients cássia veRidiana dOuRadO leMe 1 , luis séRgiO RapOsO 2 , MaRiangela tORReglOsa Ruiz 3 , jOice MatOs biselli 4 , ana lívia silva galbiatti 5 , jOsé victOR Maniglia 6 , éRika cRistina pavaRinO-beRtelli 7 , eny MaRia gOlOni-beRtOllO 8*

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Section: Discussioncontrasting

confidence: 74%

Análise dos genes GSTM1 e GSTT1 em pacientes com câncer de cabeça e pescoço

Leme¹,

Raposo²,

Ruiz³

et al. 2010

Rev. Assoc. Med. Bras.

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“…GSTP1 has a dual role in DNA damage in occupational and environmental exposure (23). There seems to be a controversy about its ability to alter the early effects of toxic exposure on DNA damage biomarkers (24). Our study confi rms this controversy.…”

Section: Discussionsupporting

confidence: 63%

Does GSTP1 Polymorphism Contribute to Genetic Damage Caused by Ageing and Occupational Exposure?

Eshkoor

Ismail

Rahman³

et al. 2011

Archives of Industrial Hygiene and Toxicology

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The aim of our study was to see the effects of GSTP1 polymorphism on biomarkers of ageing, including micronuclei (MN), comet tail length, and relative telomere length in automobile repair workers, who are exposed to a broad spectrum of potential mutagens. The analysis was performed on buccal cells collected from occupationally exposed and non-exposed (control) subjects. Samples were analysed using cytogenetic and molecular methods, including restriction fragment length polymorphism (RFLP), MN test, comet assay, and real-time PCR. The results confi rmed the DNA damaging effects of substances used in the mechanical workshops, but did not confi rm the infl uence of GSTP1 gene polymorphism on DNA damage. However, further studies on both occupationally exposed and control populations are needed to understand the relationship between GSTP1 polymorphism and genome damage.

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“…Palma and co-workers [66] found that smokers carrying the GSTM1-null genotype showed a significantly higher frequency of micronuclei (a measure of DNA damage) compared with GSTM1-positive smokers, while no such association was found in nonsmokers. Because acrolein is a substrate for GST M1-1 [64], smoking individuals with the GSTM1-null genotype may be particularly susceptible to the genotoxic and immunosuppressive effects of acrolein.…”

Section: Glutathione Conjugation With Acroleinmentioning

confidence: 99%

Acrolein: Sources, metabolism, and biomolecular interactions relevant to human health and disease

Stevens

Maier

2008

Molecular Nutrition Food Res

615

634

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Acrolein (2-propenal) is ubiquitously present in (cooked) foods and in the environment. It is formed from carbohydrates, vegetable oils and animal fats, amino acids during heating of foods, and by combustion of petroleum fuels and biodiesel. Chemical reactions responsible for release of acrolein include heat-induced dehydration of glycerol, retro-aldol cleavage of dehydrated carbohydrates, lipid peroxidation of polyunsaturated fatty acids, and Strecker degradation of methionine and threonine. Smoking of tobacco products equals or exceeds the total human exposure to acrolein from all other sources. The main endogenous sources of acrolein are myeloperoxidase-mediated degradation of threonine and amine oxidase-mediated degradation of spermine and spermidine, which may constitute a significant source of acrolein in situations of oxidative stress and inflammation. Acrolein is metabolized by conjugation with glutathione and excreted in the urine as mercapturic acid metabolites. Acrolein forms Michael adducts with ascorbic acid in vitro, but the biological relevance of this reaction is not clear. The biological effects of acrolein are a consequence of its reactivity towards biological nucleophiles such as guanine in DNA and cysteine, lysine, histidine, and arginine residues in critical regions of nuclear factors, proteases, and other proteins. Acrolein adduction disrupts the function of these biomacromolecules which may result in mutations, altered gene transcription, and modulation of apoptosis.

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Influence of glutathione S-transferase polymorphisms on genotoxic effects induced by tobacco smoke

Cited by 45 publications

References 61 publications

Análise dos genes GSTM1 e GSTT1 em pacientes com câncer de cabeça e pescoço

Análise dos genes GSTM1 e GSTT1 em pacientes com câncer de cabeça e pescoço

Does GSTP1 Polymorphism Contribute to Genetic Damage Caused by Ageing and Occupational Exposure?

Acrolein: Sources, metabolism, and biomolecular interactions relevant to human health and disease

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