Influence of genetic polymorphisms on frequency of micronucleated buccal epithelial cells in leukoplakia patients

Mahimkar, Manoj B.; Samant, Tanuja A.; Kannan, Sadhana; Patil, Tejas

doi:10.1016/j.oraloncology.2010.08.009

Cited by 36 publications

(24 citation statements)

References 28 publications

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“…However, contrary to our results, some studies reported that CYP1A1 m1 and m2 variants did not show any effect on DNA damage among coke-oven and graphide electrode workers (Marczynski et al, 2002;Chen et al, 2006;Moretti et al, 2007). Mahimkar et al (2010) found no association of CYP1A1 gene polymorphism with MN in exfoliated buccal cells in leukoplakia patients.…”

Section: Discussioncontrasting

confidence: 99%

CYP1A1 Gene Polymorphisms: Modulator of Genetic Damage in Coal-Tar Workers

Giri

Yadav²,

Kumar

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Aim: It is well known that polycyclic aromatic hydrocarbons (PAHs) such as benzo (a) pyrene have carcinogenic properties and may cause many types of cancers in human populations. Genetic susceptibility might be due to variation in genes encoding for carcinogen metabolizing enzymes, such as cytochrome P-450 (CYP450). Our study aimed to investigate the effect of genetic polymorphisms of CYP1A1 (m1 and m2) on genetic damage in 115 coaltar workers exposed to PAHs at their work place. Methods: Genetic polymorphisms of CYP1A1 were determined by the PCR-RFLP method. Comet and buccal micronucleus assays were used to evaluate genetic damage among 115 coal tar workers and 105 control subjects. Results: Both CYP1A1 m1 and CYP1A1 m2 heterozygous and homozygous (wt/mt+mt/mt) variants individually as well as synergistically showed significant association (P<0.05) with genetic damage as measured by tail moment (TM) and buccal micronuclei (BMN) frequencies in control and exposed subjects. Conclusion: In our study we found significant association of CYP1A1 m1 and m2 heterozygous (wt/mt)+homozygous (mt/mt) variants with genetic damage suggesting that these polymorphisms may modulate the effects of PAH exposure in occupational settings.

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Section: Discussioncontrasting

confidence: 99%

CYP1A1 Gene Polymorphisms: Modulator of Genetic Damage in Coal-Tar Workers

Giri

Yadav²,

Kumar

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…Several cytogenetic endpoints have been extensively used for bio-monitoring of human exposure to carcinogenic substances [26,29], with the increase in MNC being considered as predictive of an elevated cancer risk [15][16][17]30]. According to Holland et al [11], buccal epithelial cells represent a recognized target site for early genotoxic events induced by carcinogenic substances that enter the body via inhalation or ingestion.…”

Section: Discussionmentioning

confidence: 99%

“…Micronuclei in exfoliated buccal epithelial cells emerge during mitosis of the basal layers of the epithelium as extra-chromosomal DNA particles, when chromosome fragments or whole chromosomes lag behind and fail to be included in the main nuclei of the daughter cells, as a result of a clastogenic or aneugenic events [11,12]. Micronuclei in epithelial cells reflect genotoxic events occurring in the dividing basal cell layer of the putative target organ 1-3 weeks earlier [13,14], which allows for cytogenetic surveillance of groups at high risk of developing organ-specific cancer (e.g., upper aerodigestive tract) or oral premalignant lesions [15][16][17]. Chromosomal damage in epithelial cells also leads to nuclear anomalies other than micronuclei, such as karyorrhexis, karyolysis, pyknosis, condensed chromatin, 'broken eggs' and bi-nucleated cells [11,18].…”

Section: Introductionmentioning

confidence: 99%

Exposure of thermoelectric power-plant workers to volatile organic compounds from fuel oil: Genotoxic and cytotoxic effects in buccal epithelial cells

Garcia

Linhares

Amaral

et al. 2012

Mutation Research/Genetic Toxicology and Environmental Mutagene

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a b s t r a c tThermoelectric power-plant workers are constantly exposed to high levels of potentially genotoxic gaseous substances, such as volatile organic compounds (VOCs) from the combustion of fuel oil or the processing of naphtha. The aim of the present study was to estimate the association between such occupational exposure and the frequency of micronucleated cells and cells with other nuclear anomalies.Buccal epithelial cells were collected from a total of 44 power-plant workers (exposed group) and 47 administrative workers (non-exposed group), and examined for the frequency of micronucleated cells (MNC) and of cells with other nuclear anomalies (ONA: pyknosis, karyolysis, and karyorrhexis) by means of the micronucleus assay. The frequencies of MNC and ONA per 1000 cells in the exposed group (1.8‰ and 82.4‰, respectively) were significantly higher than in the non-exposed group (0.2‰ and 58.3‰, respectively). The exposed group had a twelve-fold increase in risk for formation of MNC compared with non-exposed individuals (RR = 12.1; 95% CI, 5.0-29.2; P < 0.001). The confounding factors analyzed (age, smoking status, alcohol consumption, and mouthwash use) did not show any significant association with the frequency of MNC or ONA.The findings of this study show that workers from power plants exposed to VOCs have a significantly elevated risk for DNA damage. Therefore, bio-monitoring of DNA damage is recommended for this group of workers.

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“…Several cytogenetic endpoints have been extensively used for biomonitorization of human exposure to carcinogenic substances (Ceppi et al, 2010;Knudsen and Hansen, 2007), being the increase in MNC considered predictive of elevated cancer risk (Bloching et al, 2000;Bonassi et al, 2007;Mahimkar et al, 2010;Saran et al, 2008). According to Holland et al (2008), buccal epithelial cells represent a recognized target site for early genotoxic events induced by carcinogenic substances that enter the body via inhalation or ingestion.…”

Section: Discussionmentioning

confidence: 99%

“…Micronuclei in exfoliated buccal epithelial cells emerge during mitosis of the basal layers of the epithelium as extra chromosomal DNA particles, when chromosome fragments or whole chromosomes lag behind and fail to be included in the main nuclei of the daughter cells, as a result of a clastogenic or aneugenic events (Holland et al, 2008;Tolbert et al, 1992). Micronuclei in epithelial cells reflect genotoxic events occurring in the dividing basal cell layer 1-3 weeks earlier of the putative target organ (Stich et al, 1983;Tolbert et al, 1991), which allows for cytogenetic surveillance of groups at high risk of developing organ-specific cancer (e.g., upper aerodigestive tract) or oral premalignant lesions (Bloching et al, 2000;Mahimkar et al, 2010;Saran et al, 2008). Chromosomal damage in epithelial cells also leads to nuclear anomalies other than micronuclei, such as karyorrhexis, karyolysis, pyknosis, condensed chromatin, broken eggs and binucleated cells (Holland et al, 2008;Thomas et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Evidence of DNA damage in humans inhabiting a volcanically active environment: A useful tool for biomonitoring

Rodrigues¹,

Arruda

Garcia

2012

Environment International

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This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues.Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. The present study was designed to evaluate whether chronic exposure to a volcanically active environment might result in genotoxic and cytotoxic effects in human oral epithelial cells. A study group of 120 individuals inhabiting a volcanically active environment (exposed group; Furnas village) and a reference group of 122 individuals inhabiting a village without manifestations of volcanic activity (Santo António village) were examined in this study. Individuals from Furnas village inhabit a volcanically active environment marked by several degassing manifestations, including fumarolic fields, thermal and cold CO 2 springs and soil diffuse degassing areas. For each individual, 1000 buccal epithelial cells were analyzed for the frequency of micronucleated cells (MNC) and the frequency of cells with other nuclear anomalies (ONA: pyknosis, karyolysis and karyorrhexis), by using the micronucleus assay. Information on life-style factors and an informed consent were obtained from each participant. The frequencies of MNC and of ONA per 1000 cells in the exposed group (4.3‰ and 23.5‰, respectively) were significantly higher than in the reference group (1.7‰ and 7.7‰, respectively). The risk of having a high frequency of MNC or ONA was 2.4 and 3.1-fold higher in exposed individuals compared to reference group. The analyzed confounding factors (age, gender, smoking status, alcohol consumption, and mouthwash use) did not show any significant association with the frequency of MNC or ONA. The higher risk of DNA damage in individuals inhabiting a volcanically active environment is for the first time clearly highlighted with this biomonitoring study. Given that MNC in oral epithelia are recognized as a predictive biomarker of cancer risk within a population of healthy subjects, these findings could contribute to explain the high incidence rates of lip, oral cavity and pharynx cancers previously referred for Furnas village inhabitants.

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Influence of genetic polymorphisms on frequency of micronucleated buccal epithelial cells in leukoplakia patients

Cited by 36 publications

References 28 publications

CYP1A1 Gene Polymorphisms: Modulator of Genetic Damage in Coal-Tar Workers

CYP1A1 Gene Polymorphisms: Modulator of Genetic Damage in Coal-Tar Workers

Exposure of thermoelectric power-plant workers to volatile organic compounds from fuel oil: Genotoxic and cytotoxic effects in buccal epithelial cells

Evidence of DNA damage in humans inhabiting a volcanically active environment: A useful tool for biomonitoring

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