Influence of gastrectomy for gastric cancer treatment on faecal microbiome and metabolome profiles

Abstract

<jats:sec><jats:title>Objective</jats:title><jats:p>Recent evidence points to the gut microbiome’s involvement in postoperative outcomes, including after gastrectomy. Here, we investigated the influence of gastrectomy for gastric cancer on the gut microbiome and metabolome, and how it related to postgastrectomy conditions.</jats:p></jats:sec><jats:sec><jats:title>Design</jats:title><jats:p>We performed shotgun metagenomics sequencing and capillary electrophoresis time-of-flight mass spectrometry-based metabolomics analyses on faecal samples collected from participants with a history of gastrectomy for gastric cancer (n=50) and compared them with control participants (n=56).</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>The gut microbiota in the gastrectomy group showed higher species diversity and richness (p&lt;0.05), together with greater abundance of aerobes, facultative anaerobes and oral microbes. Moreover, bile acids such as genotoxic deoxycholic acid and branched-chain amino acids were differentially abundant between the two groups (linear discriminant analysis (LDA) effect size (LEfSe): p&lt;0.05, q&lt;0.1, LDA&gt;2.0), as were also Kyoto Encyclopedia of Genes and Genomes modules involved in nutrient transport and organic compounds biosynthesis (LEfSe: p&lt;0.05, q&lt;0.1, LDA&gt;2.0).</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>Our results reveal alterations of gut microbiota after gastrectomy, suggesting its association with postoperative comorbidities. The multi-omic approach applied in this study could complement the follow-up of patients after gastrectomy.</jats:p></jats:sec>

Differential Gut Microbiota and Fecal Metabolites Related With the Clinical Subtypes of Myasthenia Gravis

“…We modified the default calculation by controlling the multiple testing using Benjamini–Hochberg (BH) false discovery rate (FDR) correction procedure. LEfSe analysis was conducted under the following conditions: p < 0.05, FDR < 0.1 and LDA > 2.5 ( Erawijantari et al, 2020 ; Zheng et al, 2020 ).…”

“…We modified the default calculation by controlling the multiple testing using Benjamini-Hochberg (BH) false discovery rate (FDR) correction procedure. LEfSe analysis was conducted under the following conditions: p < 0.05, 1 http://qiime.org/ 2 http://drive5.com/usearch/manual/uchime_algo.html 3 http://drive5.com/uparse/ 4 http://huttenhower.sph.harvard.edu/galaxy/ FDR < 0.1 and LDA > 2.5 (Erawijantari et al, 2020;Zheng et al, 2020).…”

Human Gut Microbiota: Friend or Foe?

“…In these patients, a higher abundance of aerobes, facultative anaerobes, and oral microbes was observed. The levels of F. nucleatum, a colorectal cancer-related bacterium, were significantly higher in patients who underwent total gastrectomy compared with the control samples [428]. In cancer samples, oral bacteria, such as Peptostreptococcus, Streptococcus, and Fusobacterium were predominantly detected.…”

Establishing a novel colorectal cancer predictive model based on unique gut microbial single nucleotide variant markers

“…Fecal shotgun metagenomic data of human CRC patients and healthy cohorts were collected. For discovery cohorts, raw SRA files and sample information from three studies were downloaded from NCBI using the following accessions: ERP008729 for Austria, 23 DRA006684 for Japan, 24 and SRP136711 for Italy. 25 A total of 118 cases and 131 healthy controls were included in this meta-analysis ( Table 1 ).…”