Influence of Fusarium wilt resistant tomato cultivars on root colonization of the mutualistic endophyte Fusarium oxysporum strain 162 and its biological control efficacy toward the root-knot nematode Meloidogyne incognita

Dababat, Abdelfattah A.; Selim, Mohamed E.; Saleh, Azzam; Sikora, R. A.

doi:10.1007/bf03356276

Cited by 9 publications

(4 citation statements)

References 15 publications

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“…Moreover, the virulence function has been established for tow of these small proteins using gene knockout experiments [26]. These are Six4 (Avr1), which is required for I and I-1-mediated resistance [27][28][29], and Six3 (Avr2), which is required for I-2-mediated resistance while the validity of virulence function of the other SIX genes still under investigation. In addition, Six4/Avr1 was found to suppress I-2-and I-3-mediated disease resistance [27,30,31].…”

Section: Discussionmentioning

confidence: 99%

Evaluation and Characterization of Some Egyptian Fusarium oxysporum Isolates for their Virulence on Tomato and PCR Detection of (SIX) Effector Genes

Khalifa¹

2015

J Bioprocess Biotech

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The genus Fusarium is comprises of different species which are furtherer divided into small groups called formae speciales according to their host specificity. It is remarkable that while an isolate belonging to particular formae speciales is considered highly pathogenic in a certain plant species, the many other isolates belonging to the same formae speciales may have no harm effect or even a beneficial relation to the same host plant. Traditionally, bioassays conducted under green house conditions were used to distinguish pathogenic and non-pathogenic isolates. However, such these techniques have some limitations due to the time consuming and the inconsistency of obtained results. In contrary, molecular identification techniques are candidate to play an important role in the characterization process of Fusarium isolates as well as with many other organisms. Recently, the ability of a Fusarium isolate to infect particular plant species was found to be dependent on specific genes encoding host determining 'virulence factors' that distinguish virulent from avirulent strains. More recently, eight fungal proteins were identified from xylem sap of infected plants, encompassing the small secreted proteins called Six1, Six2, Six3, Six4, Six5, Six6, Six7 and SIX 8. In the present study, the linkage between virulence potential of fifteen pathogenic isolates in addition to one non-pathogenic isolate, Fo162 , of Fusarium oxysporum and the presence of secreted in xylem effector genes (SIX) was determined. The results showed that the tested isolates were varied regarding to their pathogenicity potential toward tomato plants under greenhouse conditions. On the other hand, the amplicons of SIX1, SIX5 and SIX 7 were detected with most hyper virulent isolates while no amplicons for any tested SIX genes were observed with the non-pathogenic isolate (Fo162) as well as with the most hypo virulent isolates. These results, suggested that SIX1, SIX5 and SIX6 may play a distinct role in virulence potential of these Fusarium oxysporum isolates toward tomato plants under Egyptian conditions.

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Section: Discussionmentioning

confidence: 99%

Evaluation and Characterization of Some Egyptian Fusarium oxysporum Isolates for their Virulence on Tomato and PCR Detection of (SIX) Effector Genes

Khalifa¹

2015

J Bioprocess Biotech

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“…Alternative and environmentally compatible control strategies that use bio-based options (including resistant varieties, non-host plants, antagonistic varieties, and biological control agents) urgently need to be established before the current effective nematicides become unavailable. Among a number of different fungi that have been studied as biocontrol agents against PPNs, especially root-knot nematodes, some endophytic fungi have been shown to be promising (Dababat et al, 2008;Hallmann and Sikora, 1996).…”

Section: Introductionmentioning

confidence: 99%

Potential antagnosim of cultivated and wild grass–endophyte associations towards Meloidogyne incognita

et al. 2013

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“…Among these, the corky root disease caused by Fusarium oxysporum is observed. This fungus is one of the most important pathogens of tomato plants and other vegetable crops in protected and field conditions (Dababat et al, 2008;Scheider et al, 2009). The causal agent of fusarium wilt is soil borne pathogen which can persist many years in the soil without a host.…”

Section: Introductionmentioning

confidence: 99%

“…Biological control of F. oxysporum on numerous crops by application of antagonistic fungi and bacteria has been accomplished during last two decades all over the world (Leeman et al, 1996;Dababat et al, 2008). Antagonistic activity of biological control agents (for example Trichoderma sp.)…”

Section: Introductionmentioning

confidence: 99%

Free and encapsulated chitinase and laminarinase as biological agents against Fusarium oxysporum

Anna¹,

Erick²,

Cecilia³

et al. 2013

Afr. J. Microbiol. Res.

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In order to seek an environmentally-friendly alternative to chemical fungicide, the aims of this paper were: 1) to assess the enzymatic and antifungal activity of free and microencapsulated enzymes (laminarinase and chitinase from Trichoderma sp.) and their mixtures against F. oxysporum in soil in the presence or absence of thiabendazole; 2) to analyze remarkable variants resulting from soil testing in ad planta assay with tomato crops (L. esculentum Mill) and the pathogen F. oxysporum. To evaluate the effect of applied treatments to the fungus, the quantity of colony forming units (CFU) was determined. Enzyme activities were measured spectrophotometrically using laminarin and of p-nitrophenyl-β-D-N-acetyl-glucosamide as laminarinase and chitinase substrates, respectively. An inhibiting effect to fungus growth (fungistatic effect) of both enzymes applied separately and together, as well as the treatment with liposomes only was demonstrated in vitro and ad planta assays. Enzyme application permitted a decreasing of chemical fungicide level and allowed the achievement of partial (90-98%) or total inhibition of fungus growth. Microencapsulation led to increase enzyme stability in the presence of thiabendazole. The growth of tomato plant was higher in the presence of applied treatments. The increase of fungistatic effect was revealed in the case of mixture of encapsulated enzymes and enzymes with thiabendazole. Encapsulation of chitinase and laminarinase in soya lecithin liposomes was useful to maintain enzymatic activity for the control of phytopathogenic fungus F. oxysporum. Thus, it confirmed the idea regarding the use of free or encapsulated (in soya lecithin liposome) mycolytic enzymes for the control of phytopathogen fungus growth in the presence and absence of chemical fungicide in vitro and ad planta assays.

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Influence of Fusarium wilt resistant tomato cultivars on root colonization of the mutualistic endophyte Fusarium oxysporum strain 162 and its biological control efficacy toward the root-knot nematode Meloidogyne incognita

Cited by 9 publications

References 15 publications

Evaluation and Characterization of Some Egyptian Fusarium oxysporum Isolates for their Virulence on Tomato and PCR Detection of (SIX) Effector Genes

Evaluation and Characterization of Some Egyptian Fusarium oxysporum Isolates for their Virulence on Tomato and PCR Detection of (SIX) Effector Genes

Potential antagnosim of cultivated and wild grass–endophyte associations towards Meloidogyne incognita

Free and encapsulated chitinase and laminarinase as biological agents against Fusarium oxysporum

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