Influence of freeze –thaw and storage time on some specific human hormones

Nwankpa, Promise; Ekweogu, C.N.; Emengaha, F.C.; Ugwuezumba, P.C.; Chukwuemeka, Obinna G; Etteh, Chinedu Casmir; Egwurugwu, J. N.; Ezekwe, S. Ahamefula; Izunwanne, D. I.

doi:10.4103/njecp.njecp_14_18

Cited by 3 publications

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“…"The kidneys play a vital role in the everyday metabolism of the cell via its role in the excretion of waste products and toxins like urea, uric acid and creatinine, metabolic regulation of extracellular fluid volume, serum osmolality and electrolyte balance, coupled with the production of hormones like erythropoietin and 1,25 dihydroxy vitamin D and renin" [39,40]. "Evaluation of renal function activity is key in the diagnosis, management and treatment of patients with kidney disease and pathologies affecting renal function.…”

Section: Discussionmentioning

confidence: 99%

Positive Stimulatory Potentials of Coconut (Cocos nucifera L.) Juice Extract on in vivo Antioxidants, Renal Function and Lipid Profile of Male Wistar Rats

Kpomah

Arhoghro

2023

EJMP

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Coconut juice is a natural drink from coconut fruit. This study was aimed at evaluating its positive potential on biochemical indices. Fresh coconut fruits were de-dshelled and washed. The cotyledon was broken to extract the water which was used in blending the fruits. The extract was filtered and the filtrate was collected in a vial. Twenty healthy male Wistar rats weighing 160 -180 g, were grouped into two groups of ten rats each and were treated as follows for four weeks. Group A: control rats received oral dose of 2 mL/kg body weight of distilled water once daily for 28 days, and group B: received oral dose of 2 mL/kg body weight of coconut juice extract once daily for 28 days, both groups were, however, allowed free access to feed and water ad-libitum. The animals were weighed before and on completion of the experimental protocol. Blood was also collected via cardiac puncture and used for renal function and lipid profile assay, kidneys were excised and weighed, homogenized and the supernatant was used for antioxidant enzyme evaluation. Findings showed that there was no significant change (p>0.05) in the kidney-to-body weight ratio and renal function indices. Significant reduction (p<0.05) was seen in cholesterol, triglycerides, LDL but with an increase (p<0.05) in HDL. Antioxidant enzymes were significantly increased (p<0.05). The study concludes that coconut juice extract has positive stimulatory potentials on antioxidants, renal functions and lipid parameters of wistar rats.

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Section: Discussionmentioning

confidence: 99%

Positive Stimulatory Potentials of Coconut (Cocos nucifera L.) Juice Extract on in vivo Antioxidants, Renal Function and Lipid Profile of Male Wistar Rats

Kpomah

Arhoghro

2023

EJMP

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“…With regard to sample stability, neither fecal freeze‐thaw cycle nor fecal extract storage temperature affect DHEAS concentrations. Most studies have reported no changes in steroid hormone concentrations of serum samples after a few freeze‐thaw cycles for humans (Hillebrand et al, 2017; Livesey et al, 1980; Nwankpa et al, 2018) and nonhuman mammals (Gholib et al, 2019). One study using a C18 cartridge to extract fecal steroids from geladas ( Theropithecus gelada ) reported that two and six freeze‐thaw cycles were necessary to affect fecal GC and fecal testosterone levels, respectively (Pappano et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Validation of an enzyme immunoassay for measurement of fecal dehydroepiandrosterone sulfate in gibbons and siamangs

Takeshita

2022

Zoo Biology

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Monitoring wildlife stress levels is essential to ensure their quality of life in captivity or in the wild. One promising method to assess the stress response is the comeasurement of glucocorticoids (GC) and dehydroepiandrosterone sulfate (DHEAS), adrenal hormones involved in the modulation of the stress response. Although noninvasive methods to measure GCs have been validated in several species, only a few studies have validated DHEAS assays. The aims of this study were (1) to describe an enzyme immunoassay (EIA) to measure DHEAS levels, (2) to validate this assay for fecal samples in gibbons and siamangs, and (3) to test hormonal stability after one freeze-thaw cycle and over time at two freezer temperatures (−20°C and −80°C). Subjects included 32 gibbons and siamangs from U.S. zoological parks. The EIA was validated analytically by parallelism and accuracy tests, and biologically by confirming a DHEAS response 1-2 days after a stressful event (accident, vaccination, or transportation) in three individuals. In addition, fecal DHEAS levels in a pregnant female were above nonpregnant/nonlactating levels and declined progressively the following parturition. The hormonal stability experiments revealed no significant changes in fecal DHEAS levels after one freeze-thaw cycle.Hormonal levels in fecal extracts were stable for 2 months, regardless of the storage temperature, with no significant differences between −20°C and −80°C conditions. The EIA described has high sensitivity and it is suitable for fecal DHEAS measurement in gibbons and siamangs, with a potential to be applied to other species.

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Stability of some electrolytes and thyroid hormone concentrations in repeated freeze-thaw serum of Karadi sheep

Mohammed

Ahmed

et al. 2021

TJAS

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This study aimed to evaluate the effect of repeated freeze-thaw cycles on some electrolytes’ concentration (Na+, K+, Ca+ and Cl−) and thyroid hormones (T3 and T4), in the serum of Karadi sheep breed. This study was conducted on 18 male and female sheep (aged over one year) in two lines of Karadi sheep Jeshana and Jaff in Sulaimaniyah province. Blood samples were collected from each animal and allowed to clot for 45 to 60 min, then centrifuged at 3000 × g for 10 min to separate the serum. The serum sample was frozen at (-40) degrees and was monitored for 18 months. Serum sample analyzed after (18 months) of storage. Our result showed that repeated freeze-thaw cycling has significant and relevant increases of serum T4 (Thyroxine hormone) in both male and female groups without affecting T3 (Triiodothyronine hormone). Na+ and Cl- in both male and female groups showed a significant difference compared to the control group while the remaining electrolytes K+ and Ca+ didn’t show any relevant changes. This study has demonstrated that repeated freeze-thaw cycles do not cause changes in some biochemical constituents studied in sheep serum.

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Influence of freeze –thaw and storage time on some specific human hormones

Cited by 3 publications

References 0 publications

Positive Stimulatory Potentials of Coconut (Cocos nucifera L.) Juice Extract on in vivo Antioxidants, Renal Function and Lipid Profile of Male Wistar Rats

Positive Stimulatory Potentials of Coconut (Cocos nucifera L.) Juice Extract on in vivo Antioxidants, Renal Function and Lipid Profile of Male Wistar Rats

Validation of an enzyme immunoassay for measurement of fecal dehydroepiandrosterone sulfate in gibbons and siamangs

Stability of some electrolytes and thyroid hormone concentrations in repeated freeze-thaw serum of Karadi sheep

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