Influence of Frame Size and Zeranol on Growth, Compositional Growth and Plasma Hormone Characteristics

Williams, Justin; Sj, Miller; Ta, Mollett; Se, Grebing; Dk, Bowman; Mr, Ellersieck

doi:10.2527/jas1987.6541113x

Cited by 23 publications

(18 citation statements)

References 14 publications

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“…Percent recovery was analyzed by spiking the standards of each kit with plasma in a 1:30 standard:plasma dilution. The kits were validated by performing curve displacement and parallelism by diluting plasma samples in a 1:1 ratio with each standard and plotting the final value against standard curves (Williams et al, 1987). The limits of detection for T3 and T4 assays were 10 ng/mL and 0.4 µg/dL, respectively; intra-assay CV for T3 and T4 were 5% and 6%, and interassay CV were 8% and 7%, respectively.…”

Section: Endocrine Markersmentioning

confidence: 99%

Impact of cow size on dry matter intake, residual feed intake, metabolic response, and cow performance

Walker

Martin

Gentry

et al. 2015

Journal of Animal Science

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Thirty-eight Angus-cross beef cows were used to evaluate differences in DMI, residual feed intake (RFI), and endocrine markers on the basis of cow size and RFI ranking during 2 stages of production. Cows housed in individual pens (2.2 × 9.1 m) were fed, over a 70-d feeding period, 30% Bermuda grass hay and 70% ryegrass baleage diet during lactation (LACT) and a 100% ryegrass hay diet during postweaning (NOLACT). Individual daily feed intake, BW, and BCS were recorded, and hip height was used to determine frame score (FS). Feed intake was used to calculate RFI for each cow, and cow was the experimental unit. Blood samples were obtained on d 0 and 70 and were analyzed for glucose, insulin, leptin, triiodothyronine (T3), and thyroxine (T4). Cows were assigned to a light (LIT) or heavy (HEV) BW groups on the basis of mean BW at the beginning of the LACT period. On the basis of RFI values for each feeding period, cows were placed into a negative (NEG; RFI < 0.00) or positive (POS; RFI > 0.00) RFI group and into a low (LOW; ≤0.2 SD mean RFI), medium (MED; within ±0.19 SD), or high (HI; ≥0.2 SD mean RFI) RFI group. During LACT, DMI was 4.8% greater (P = 0.03) and FS was greater (P < 0.01; 6.4 and 5.5 ± 0.16) for the HEV compared with LIT BW cows. No RFI by day interaction or RFI group main effect occurred for endocrine markers during LACT; however, a negative relationship (P = 0.04) existed between BW group and combined T3 data, and a positive relationship (P = 0.04) existed between RFI and combined insulin data. For both LACT and NOLACT, RFI was similar (P > 0.05) among BW groups; however, DMI was 6.5% and 8.9% greater (P < 0.01) for POS compared with NEG RFI in the LACT and NOLACT periods. In LACT, DMI was greater (P < 0.01) for HI and MED RFI compared with LOW RFI, and in NOLACT, DMI was greater (P < 0.01) for the HI compared with MED and LOW RFI cows and MED compared with LOW RFI cows. During NOLACT, DMI was 8.9% greater (P < 0.01) for the HEV (12.4 ± 0.22 kg) compared with LIT (11.3 ± 0.19 kg) BW cows. Change in BCS was greater (P ≤ 0.03) in higher RFI cows in both RFI groups only in the NOLACT period. Differences in T3 and T4 on d 0 and 70 were 25% and 15% greater (P ≤ 0.04) for the LIT BW group compared with the HEV BW group. A negative correlation existed (P ≤ 0.04) between BW group and T3 and T4, as well as leptin and RFI (P = 0.03). Although cow BW was independent of RFI and T3 and T4 levels tended to be greater in lighter BW cows, DMI was consistently greater for cows with heavier BW and higher RFIvalues.

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Section: Endocrine Markersmentioning

confidence: 99%

Impact of cow size on dry matter intake, residual feed intake, metabolic response, and cow performance

Walker

Martin

Gentry

et al. 2015

Journal of Animal Science

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“…Insulin, cortisol, T3, T4 GH concentrations were determined by radioimmunoassay using commercial kits (Coat-A-Count, Diagnostic Products Corporation, Los Angeles, CA, USA; Williams et al, 1987). The sensitivity of the insulin assay was 1.0 mU L -1 ; the intra-and interassay coefficients of variation were 8.25 and 8.81%, respectively.…”

Section: Methodsmentioning

confidence: 99%

Growth, blood metabolites and hormones in calves fed diets with different amounts of energy and protein during the pre- or post-weaning periods

Fernández¹

2012

Afr. J. Agric. Res.

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We investigated the effects of diets differing in energy and protein concentrations given during pre-and post-weaning on hormone and metabolite levels in association to parameters of growth. Sixteen Aberdeen Angus calves four-day-old were allocated in individual pens during 105 days in a randomized complete design. Animals consumed ad libitum various diets differing in proportion of energy and protein (A: 80% of concentrate, 20% alfalfa hay; B: 60% concentrate, 40% of alfalfa hay; C: 40% concentrate, 60% alfalfa hay, and D: 20% concentrate, 80% alfalfa hay). Also, calves consumed milk substitute up to 60-day-old day. Serum growth hormone and cortisol levels were not altered with diet changes (P > 0.05) neither during the pre-nor the post-weaning periods. Plasma insulin concentration average daily intake, glucose and thyroid hormone levels, and concentrations of ammonia nitrogen and propionic acid were greater (P < 0.01) for diets having higher than lower energy and protein levels during the post-weaning but not during the pre-weaning period. These results suggest inclusion of higher amounts of concentrate in the diet of weaned calves.

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“…An RIA b that has been validated for the analysis of hormones in human and in animal samples 3,5,14,15 was used as the reference method. The performances of the nonradioactive methods were compared with the RIA for samples obtained from different animal species.…”

mentioning

confidence: 99%

Validation of Nonradioactive Chemiluminescent Immunoassay Methods for the Analysis of Thyroxine and Cortisol in Blood Samples Obtained from Dogs, Cats, and Horses

et al. 1997

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Abstract. The performances of a radioimmunoassay method, a chemiluminescent immunoassay method, and a chemiluminescent-enzyme immunoassay method were evaluated for the analysis of cortisol and total thyroxine in blood samples obtained from dogs, cats, horses, and humans (reference samples). The analysis of cortisol in human and animal samples exhibited good precision, linearity, and recovery. The 3 methods gave comparable values for the ACTH-induced increase and the dexamethasone-induced decrease in cortisol concentrations in animal samples. The recoveries of total thyroxine from human samples, analyzed by the 3 methods, were comparable. However, the basal total thyroxine concentrations determined by the chemiluminescent immunoassay method were 30-40% lower than those determined by the radioimmunoassay and the chemiluminescent-enzyme immunoassay methods in animal samples. In both human and animal samples, the plot of thyroxine values obtained by the radioimmunoassay method against those obtained by the chemiluminescent immunoassay method or the chemiluminescent-enzyme immunoassay method was linear. However, although the slope of the radioimmunoassay versus chemiluminescent-enzyme immunoassay curve was close to unity, the slope of the radioimmunoassay versus chemiluminescent immunoassay curve was 0.6. This result suggests that, compared with the radioimmunoassay method, the chemiluminescent immunoassay method underestimated thyroxine values in animal samples but not in human samples. Although all 3 methods yielded comparable changes in thyroxine concentrations in response to thyroid stimulating hormone, they did not yield comparable thyroxine concentrations in response to T 3 suppression in dogs and cats.The measurement of hormones in plasma or serum is commonly made by radioimmunoassay (RIA) using 125 I or 3 H-labeled hormones. RIA is a simple, rapid, and sensitive method for the measurements of thyroxine (T 4 ) and cortisol in both human and animal samples. The use of radioactive isotopes, however, requires appropriate licensing, storage facility, safety devices, and record keeping that increase the operating cost. Recently, several nonradioactive immunoassay methods such as a chemiluminescent immunoassay (CIA) a and a chemiluminescent-enzyme-immunoassay (CEIA) b methods have been developed for the analysis of hormones in blood samples. The detection limits reported for the nonradioactive methods have been reported to be comparable to or better than those for RIA methods.2 The nonradioactive methods, however, have been validated only for human samples, and veterinary use of these assays has not yet been established.The diagnosis of thyroid and adrenocortical disor- Received for publication May 24, 1996. 261 ders in animals requires appropriate analytical tests of pituitary, thyroid, and adrenal cortex. The plasma or serum concentrations of hormones 8,11 are widely used to diagnose thyroid and adrenocortical diseases in animals. An abnormality in the regulation of the thyroid gland by the pituitary can be d...

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Influence of Frame Size and Zeranol on Growth, Compositional Growth and Plasma Hormone Characteristics

Cited by 23 publications

References 14 publications

Impact of cow size on dry matter intake, residual feed intake, metabolic response, and cow performance

Impact of cow size on dry matter intake, residual feed intake, metabolic response, and cow performance

Growth, blood metabolites and hormones in calves fed diets with different amounts of energy and protein during the pre- or post-weaning periods

Validation of Nonradioactive Chemiluminescent Immunoassay Methods for the Analysis of Thyroxine and Cortisol in Blood Samples Obtained from Dogs, Cats, and Horses

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