Influence of formalin fixation on the implant stability quotient and mechanical characteristics of bone

Morita, Koji; Doi, Katsuhiko; Oue, Hiroshi; Kajihara, Shiho; Hayashi, Kazuhiko; Akagawa, Yasumasa

doi:10.1016/j.bjoms.2012.08.009

Cited by 15 publications

(6 citation statements)

References 22 publications

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“…Removal torque test: Both tibias of the lot of five animals, previously designed for the biomechanics test, were removed and processed immediately after the euthanasia for the measurement of the maximum removal torque of each implant to conserve the mechanical proprieties of the bone [25]. A similar procedure compared to other studies was performed by our group [26].…”

Section: Methodsmentioning

confidence: 99%

Biomechanical and Histological Analysis of Titanium (Machined and Treated Surface) Versus Zirconia Implant Materials: An In Vivo Animal Study

et al. 2019

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Objectives: The aim of this study was to perform an in vivo histological comparative evaluation of bone formation around titanium (machined and treated surface) and zirconia implants. For the present study were used 50 commercially pure titanium implants grade IV, being that 25 implants with a machined surface (TiM group), 25 implants with a treated surface (TiT group) and, 25 implants were manufactured in pure zirconia (Zr group). The implants (n = 20 per group) were installed in the tibia of 10 rabbits. The implants distribution was randomized (n = 3 implants per tibia). Five implants of each group were analyzed by scanning electron microscopy and an optical laser profilometer for surface roughness characterization. Six weeks after the implantation, 10 implants for each group were removed in counter-torque for analysis of maximum torque value. The remaining samples were processed, included in historesin and cut to obtain non-decalcified slides for histomorphological analyses and histomorphometric measurement of the percentage of bone-implant contact (BIC%). Comparisons were made between the groups using a 5% level of significance (p < 0.05) to assess statistical differences. The results of removal torque values (mean ± standard deviation) showed for the TiM group 15.9 ± 4.18 N cm, for TiT group 27.9 ± 5.15 N cm and for Zr group 11.5 ± 2.92 N cm, with significant statistical difference between the groups (p < 0.0001). However, the BIC% presented similar values for all groups (35.4 ± 4.54 for TiM group, 37.8 ± 4.84 for TiT group and 34.0 ± 6.82 for Zr group), with no statistical differences (p = 0.2171). Within the limitations of the present study, the findings suggest that the quality of the new bone tissue formed around the titanium implants present a superior density (maturation) in comparison to the zirconia implants.

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Section: Methodsmentioning

confidence: 99%

Biomechanical and Histological Analysis of Titanium (Machined and Treated Surface) Versus Zirconia Implant Materials: An In Vivo Animal Study

et al. 2019

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“…A close look at the literature shows that formalin causes changes in the organic matrix with irreversible alteration of material and mechanical properties 9,10 . Even immediate impairment (after 12 h) of biomechanical properties is described when using formaldehyde 19 or ethanol and therefore both substances are not recommended if material characteristics close to the in-vivo conditions are of interest 9 .…”

Section: Discussionmentioning

confidence: 99%

“…Regarding formalin and ethanol based storage methods, negative effects are described in the literature. Formalin based methods reveal changes of the organic matrix with irreversible alteration of material and mechanical properties caused by formalin, especially after storage periods over eight weeks 9,10 . Even immediate impairment of biomechanical properties is described when using ethanol 9 .…”

Section: Introductionmentioning

confidence: 99%

Effect of two (short-term) storage methods on load to failure testing of murine bone tissue

Tiefenboeck

Payr

Bajenov

et al. 2019

Sci Rep

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Since mechanical testing of bone quality is often delayed following euthanasia, the method of bone storage is of high importance in animal studies. Different storage methods may cause a change in the properties of bone tissue during mechanical testing. Therefore, the aim of this study was to investigate the biomechanical effects of two different fixation methods for bone tissue. We hypothesized that there is a difference between the load to failure values between the two groups. The tibias of fifteen 18-week-old female C57BL/6 mice were harvested and randomly allocated to three different groups with varying storage methods: (1) frozen at −80 °C, (2) paraformaldehyde working solution, and (3) native group. A storage time of two weeks prior to testing was chosen for groups 1 and 2. In group 3, referred to as the “native group”, bones were immediately tested after the harvesting procedure. The comparison of the mean load to failure of all 3 groups (group 1: 28.7 N ± 6.1 N, group 2: 23.8 N ± 3.8 N and group 3: 23.7 N ± 5.7 N) did not reveal a significant difference. There was also no difference in strength or stiffness. The findings of the present study demonstrate that the two most common storage methods, do not have an influence on the biomechanical properties of murine bone over a two week period.

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“…It is not yet clear how fixation with formalin influences ISQ values and the mechanical characteristics of bone. However, Morita and coworkers, using a rabbit tibia model, observed that formalin fixation appears to affect bone mechanical characteristics by binding amino-proteins and forming bridges between the formalin and protein, which potentially influences three-dimensional protein structure, whereas it does not appear to influence ISQ values [33]. …”

Section: Discussionmentioning

confidence: 99%

AnEx VivoModel in Human Femoral Heads for Histopathological Study and Resonance Frequency Analysis of Dental Implant Primary Stability

Hernández‐Cortés

Monje

Galindo‐Moreno

et al. 2014

BioMed Research International

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Objective. This study was designed to explore relationships of resonance frequency analysis (RFA)—assessed implant stability (ISQ values) with bone morphometric parameters and bone quality in an ex vivo model of dental implants placed in human femoral heads and to evaluate the usefulness of this model for dental implant studies. Material and Methods. This ex vivo study included femoral heads from 17 patients undergoing surgery for femoral neck fracture due to osteoporosis (OP) (n = 7) or for total prosthesis joint replacement due to severe hip osteoarthrosis (OA) (n = 10). Sixty 4.5 × 13 mm Dentsply Astra implants were placed, followed by RFA. CD44 immunohistochemical analysis for osteocytes was also carried out. Results. As expected, the analysis yielded significant effects of femoral head type (OA versus OA) (P < 0.001), but not of the implants (P = 0.455) or of the interaction of the two factors (P = 0.848). Bonferroni post hoc comparisons showed a lower mean ISQ for implants in decalcified (50.33 ± 2.92) heads than in fresh (66.93 ± 1.10) or fixated (70.77 ± 1.32) heads (both P < 0.001). The ISQ score (fresh) was significantly higher for those in OA (73.52 ± 1.92) versus OP (67.13 ± 1.09) heads. However, mixed linear analysis showed no significant association between ISQ scores and morphologic or histomorphometric results (P > 0.5 in all cases), and no significant differences in ISQ values were found as a function of the length or area of the cortical layer (both P > 0.08). Conclusion. Although RFA-determined ISQ values are not correlated with morphometric parameters, they can discriminate bone quality (OP versus OA). This ex vivo model is useful for dental implant studies.

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Influence of formalin fixation on the implant stability quotient and mechanical characteristics of bone

Cited by 15 publications

References 22 publications

Biomechanical and Histological Analysis of Titanium (Machined and Treated Surface) Versus Zirconia Implant Materials: An In Vivo Animal Study

Biomechanical and Histological Analysis of Titanium (Machined and Treated Surface) Versus Zirconia Implant Materials: An In Vivo Animal Study

Effect of two (short-term) storage methods on load to failure testing of murine bone tissue

AnEx VivoModel in Human Femoral Heads for Histopathological Study and Resonance Frequency Analysis of Dental Implant Primary Stability

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